Antimicrobial Resistance and Plasmid Profiling of ESBL Producing Klebsiella spp. of Bovine Origin of Eastern Plain Zone of Uttar Pradesh
DOI:
https://doi.org/10.48165/ijvsbt.19.5.11Keywords:
Bovine, ESBLs, Klebsiella spp, MDR, PlasmidAbstract
Emergence of antimicrobial resistance in animal husbandry is of utmost concern as it has resulted in therapeutic failure and nowadays it is one of the latest challenges faced by scientific community across the world. Extensive use of antibiotics without performing antibiotic susceptibility testing may lead to development of multidrug resistant (MDR) organisms. This study was undertaken to assess the AMR pattern and their correlation with plasmid profiling in ESBL producing Klebsiella spp. from bovine origin. Total 160 samples were collected from 2 district of Eastern Plain Zone of Uttar Pradesh (India) and 63 (39.37%) isolates were confirmed as Klebsiella spp. by conventional methods (cultural characteristics on selective media & biochemical tests). Antimicrobial susceptibility testing of confirmed isolates revealed 82.0%-94.0% resistance to 3rd & 4th generation cephalosporin, 100% to ampicillin and all isolates were found 100% sensitive to aminoglycosides, chloramphenicol and polypeptides class of antibiotics. Total 49 (77.8%) isolates were confirmed as ESBL producers using DDST and ESBL E-strip test, out of which 45 (71.43%) isolates revealed the presence of bla-CTX-M gp genes by PCR analysis. On plasmid profiling, total 82.82% isolates revealed the presence of plasmid with molecular weight between 2.5 kb to >10 kb and number 1-2. These high molecular weight plasmids are mostly situated on conjugative plasmid, which are horizontally transferred from one species to another in highly populous country like India.
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Badri, A.M., Ibrahim, T.I., Mohamed, S. ., Garbi, M. ., Kabbashi, A., & Arbab, M.H. (2017). Prevalence of ESBL producing E. coli and K. pneumoniae isolated from raw milk samples in Al Jazirah State, Sudan. Molecular Biology: Open Access, 7(1), 1-4.
CLSI (2019). Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 29th Informational Supplement. CLSI document M100-S29. Wayne, PA, USA.
Dallenne, C., Costa, D.A., Decre, D., Favier, C., & Arlet, G. (2010). Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. Journal of Antimicrobial Chemotherapy, 65(3), 490-495.
Koovapra, S., Bandyopadhyay, S. Das, G., Bhattacharya, D., Banerjee, J., Mahanti, A., Samanta, P.K., Nanda, P.K., Kumar, A., Mukherjee, R., Dimri, U., & Singh, R.K. (2016). Molecular signature of ESBL-producing Klebsiella pneumoniae isolated from bovine milk in eastern and north-eastern India. Infection, Genetics and Evolution, 44, 395-402.
Mansour, M.A., Zaki, H.M., Hassan, N.A., & Al-Humiany, A.A. (2014). Molecular characterization and immune-protective activity of capsular polysaccharides of Klebsiella pneumoniae isolated from farm animals at Taif Governorate. American Journal of Infectious Diseases, 10(1), 1-14.
Olowe, O.A., Adewumi, O., Odewale, G., Ojurongbe, O., & Adefioye, O.J. (2015). Phenotypic and molecular characterization of Extended-spectrum β-lactamase producing E. coli obtained from animal faecal samples in Ado Ekiti, Nigeria. Journal of Environmental and Public health, 2015, ID 497980, http://dx.doi.org/10.1155/2015/ 497980.
Rawat, N., Singh, F., Hirpurkar, S.D., Sannat, C., & Gade, N.E. (2018). Detection and characterization of extended spectrum β-lactamase genes (blaTEM and blaSHV) among β-lactam-resistant faecal coliforms of dairy cattle from Chhattisgarh, India. Turkish Journal of Veterinary and Animal Sciences, 42, 503-511.
Reuland, E.A., Overdevest, I.T., Al Naiemi, N., Kalpoe, J.S., Rijnsburger, M.C., Raadsen, S.A., Ligtenberg-Burgman, I., vanderZwaluw, KW., Heck, M., & Savelkoul, P.H., et al. (2013). High prevalence of ESBL-producing Enterobacteriaceae carriage in Dutch community patients with gastrointestinal complaints. Clinical Microbiology and Infection, 19(6), 542-549.
Rozwannowicz, M., Brouwer, M.S.M., & Fischer, J., et al. (2018). Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae. Journal of Antimicrobial Chemotherapy, 73(5), 1121-1137.
Saini, V, McClure, J.T., Leger, D., & Morck, D.W. (2012). Antimicrobial resistance profiles of common mastitis pathogens on Canadian dairy farms. Journal of Dairy Science, 95, 4319-4332.
Salauddin, M., Akter, M.R., Hossain, M.K., & Rahman, M.M. (2019). Isolation of multi-drug resistant Klebsiella spp. from bovine mastitis samples in Rangpur, Bangladesh, Journal of Advanced Veterinary and Animal Research, 6(3), 362-365.
Singh, F., Hirpurkar, S.D., Rawat, N., Shakya S., Kumar, R., Rajput, P.K., & Kumar, S. (2020). Occurrence of the genes encoding carbapenemases, ESBLs and class 1 integron-integrase among fermenting and non-fermenting bacteria from retail goat meat. Applied Microbiology, 71(6), 611-619.
Singh, F., Hirpurkar, S.D., Shakya, S., Rawat, N., Devangan, P., Khan, F.F., & Bhandekar, S.K. (2017). Presence of enterobacteria producing Extended-spectrum β-lactamases and/or carbapenemases in animals, humans and environment in India. Thai Journal of Veterinary Medicine, 47, 35-43.
Yadav, A., Joshi, N., & Joshi, R.K. (2019). Occurrence of ESBLs producing Enterobacteria in animal products and their environments. International Journal of Current Microbiology and Applied Sciences, 8(5), 2255-2264.
Yadav, V., Joshi, R.K., Joshi, N., Kumar, A., Singh, S.V., & Niyogi, D. (2022a). Detection and molecular characterization of Extended-spectrum β-lactamase producing E. coli & Klebsiella spp. isolates of cattle origin in Eastern Plain Zone of Uttar Pradesh. Indian Journal of Animal Research, 56(11), 1369-1376.
Yadav, V., Joshi, R.K., Joshi, N., Singh, S.V., Gupta, R.K., Niyogi, D., & Yadav, D.K. (2022b). Prevalence of Extended-spectrum β-lactamase genes (bla-CTX-M, bla-TEM and bla-SHV) in E. coli and Klebsiella spp. isolated from buffaloes in Eastern Plain Zone of Uttar Pradesh. Indian Journal of Animal Research, DOI: 10.18805/IJAR.B-5036.
Yadav, V., Joshi, R.K., Joshi, N., Singh, S.V., Maurya, S.K., Srivastva, D.P., & Gupta, R.K. (2023). Status of MDR and plasmid profiling of ESBL producing E. coli and Klebsiella spp. isolated from milk of bovine in Gangetic Plain Zone of Uttar Pradesh. Indian Journal of Veterinary Science & Biotechnology, 19(2), 16-22.
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