Effect of Trehalose on Cryopreservation of Hariana Bull Spermatozoa

Authors

  • Gyanesh Kumar Department of Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Dileep Kumar Yadav Department of Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Atul Saxena Department of Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Vikas Sachan Department of Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Anuj Kumar Department of Gynaecology and Obstetrics, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Dilip Kumar Swain Department of Veterinary Physiology, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Brijesh Yadav Department of Veterinary Physiology, College of Veterinary Sciences and Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India

DOI:

https://doi.org/10.48165/ijvsbt.19.6.02

Keywords:

Antioxidant, Cryopreservation, Freezability, Hariana bull, Trehalose

Abstract

The present experiment was designed to study the effect of supplementation of Trehalose on freezability and antioxidant activity of Hariana bull semen. Semen samples were diluted in TFYG extender containing different concentrations of Trehalose (10mM, 30mM & 50mM). The control samples were extended with TFYG alone.  Results clearly indicated that, 30 mM Trehalose group had significantly (P<0.05) higher percentage of individual motility, sperm viability, & higher percentage of intact acrosome in comparison to the control and other Trehalose supplemented groups. In biochemical assays, no clear-cut demarcation for antioxidant enzyme was found for Trehalose supplementation. However, Trehalose supplementation has showed increase activity for Glutathion reductase (GR). The results obtained clearly indicated that supplementation of Trehalose to extender prior to cryopreservation improves Hariana bull sperm quality.

Downloads

Download data is not yet available.

References

Aisen, E.G., Quintana, M., Medina, V., Morello, H. and Venturino, A. (2005). Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose - based hypertonic extenders. Cryobiology, 50:239–249.

Aisen, E.G., Alvarez, H.L., Venturino, A. and Garde, J.J. (2000). Effect of trehalose and EDTA on cryoprotective action of ram semen diluents. Theriogenology, 53:1053-1061

Aisen, E.G, Cisale, H. and Fernandez, H. (1990). Criopreservación de semen ovino. Nueva técnica (in Spanish) Veterinary. Argentina, 63:176–182.

Aisen, E.G., Medina, V.H. and Venturino, A. (2002). Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57:1081-1088.

Aitken, R.J, and Fisher, H. (1994). Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays, 16:259–67.

Al-Badrany, Q.M, Al-Badry, K.I, Ibrahim, F.F. and Lateef, W.Y. (2017). Effect of addition trehalose and steps of freezing on sperm properties of bull frozen in liquid nitrogen. Int Jour. of Adv. Res. in Bio. Sci., 4:189-200.

Alvarez, J.G, Storey, and B.T. (1989). Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Research, 23:77–90.

Alvarez, J.G. and Storey, B.T. (1995). Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol. Repro. and Devel., 42:334–46.

Andrabi, S.M.H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Repr. in Domet. Anim., 44:552–69.

Anzar, M., Rasul, Z., Ahmed, T.A. and Ahmad, N. (2010). Response of buffalo spermatozoa to low temperatures during cryopreservation. Reproduction, Fertility and Development, 22: 871-880.

Armstrong, J.S., Rajasekaran, M., Hellstrom, W.J. and Sikka, S.C. (1998). Antioxidant potential of human serum albumin: role in the recovery of high quality human spermatozoa for assisted reproductive technology. Jour. of Andro., 19:412-419.

Badr, M.R., Malak, M.G., Hassan, and M.H. (2010). Effect of Trehalose on cryopreservation, oxidative stress and DNA integrity of buffalo spermatozoa. Jour. of Reprod. and Ferti., 1(2): 50-57.

Bailey, J.L., Morrie, A. and Cormier, N. (2003). Semen cryopreservation: success and persistent in farm species. Cana. J. of Anim. Sci., 83:393-401.

Batellier, F., Vidament, M., Fauquant, J., Duchamp, G., Arnaud, G., Yvon, J.M. and Magistrini, M. (2001). Advances in cooled semen technology. Anim. Reprod. Sci., 68:181-190.

Bloom, E. (1950). A one minute live-dead sperm stain by means of eosin-nigrosin. Fertility and Sterility, 1:176-177.

Bucak, M.N, Ateşşahin, A., Varışl, O., Yuce, A., Tekin, N. and Akcay, A. (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: Microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 67:1060–1067.

Chatterjee, S., Lamirande, E.D. and Gagnon, C. (2001). Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione. Mole. Reprod. and Devel., 60:498–506.

Chhillar, S., Singh, V.K., Kumar, R., and Atreja, S.K. (2012). Effects of Taurine or Trehalose supplementation on functional competence of cryopreserved Karan Fries semen. Anim Reprod. Sci., 135:1-7.

Crowe, J.H., Crowe, L.M., Carpenter, J.F. and Wistrom, C.A. (1987). Stabilization of dry phospholipid bilayers and proteins by sugars. Bioch. Jourl., 242:1–10.

Dalimata, A.M and Graham, J.K. (1997). Cryopreservation of rabbit spermatozoa using acetamide in combination with trehalose and methyl cellulose. Theriogenology., 48: 831-841.

Diya, A., El-Badry., Amal, M., Maaty, A.E.L, Gamal, A. and Sisy, E.L. (2017). The Effect of Trehalose Supplementation of INRA-82 Extender on Quality and Fertility of Cooled and Frozen-Thawed Stallion Spermatozoa. J. of Equi. Vete. Sci., 48: 86–92.

Duncan, D.B. (1955). Multiple Range and Multiple F- Tests. Biometrics, 11, 1-42.

England, G.C. (1993). Cryopreservation of dog semen: a review. J. of Reprod. and Ferti., 47:243-55.

Fernandez, S.M.R., Martinez, P.F., Garcia, M.V., Estes, M.C., Soler, A.J. and Paz, P. et al. (2007). Extender osmolality and sugar supplementation exert a complex effect on the cryopreservation of Iberian reed deer (cervus elaphus hispanicus) epididymal spermatozoa. Theriogenology, 67:738-753.

Fiser, P.S., Ainsworth, L. and Fairfull, R. (1987). Evaluation of a new diluent and different processing procedures for cryopreservation of ram semen. Theriogenology, 28:599–607.

Gutierrez, P.O., Juarez, M.M., Carvajal, S.M. and Ortega, M.E. (2009). Boar spermatozoa cryo preservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58:287-292.

Gutierrez, P.O., Juarez, M.M., Carvajal, S.M. and Ortega, M.E. (2009). Boar spermatozoa cryo preservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58:287-292.

Hancock, J.L. (1951). A staining technique for the study of temperature shock in semen. Nature (London), 169:323- 326.

Holt, W.V. (2000). Basic aspects of frozen storage of semen. Animal Reproduction Science, 62:3-22.

Holt WV. (2000). Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology, 53:47-58.

Hu, J.H, Li, Q.W., Jiang, Z.L., Yang, H., Zhang, S.S. and Zhao, H.W. (2009). The Cryoprotective Effect of Trehalose Supplementation on Boar Spermatozoa Quality. Reprod. in Dome. Anim., 44:571-575.

Hu, J.H., Zan, L.S., Zhao, X.L., Li, Q.W., Jiang, Li, Y.K. and Li, X. (2010). Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen. J. of Anim. Sci., 88:1657-1662.

Iqbal, S., Murtaza, S., Hassan, A., Amjad, R., Durrani, A.Z. and Ahmad, N. (2016). Trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in Nili Ravi buffaloes (Bubalus bubalis). Theriogenology, 85:954–959.

Kumar, R., Jagan., Mohanarao, G., Arvind. and Atreja, S.K. (2011). Freeze–thaw induced genotoxicity in buffalo (Bubalus bubalis) spermatozoa in relation to total antioxidant status. Mole. Biol. Reports., 38:1499–1506.

Kumar, S., Millar, J.D. and Waston, P.F. (2003). The effect of cooling rate on the survival of cryopreserved bull, ram, and boar spermatozoa: a comparison of two controlled-rate cooling machines. Cryobiology, 46:246–253.

Lenzi, A., Gandini, L., Lombardo, F., Picardo, M., Maresca, V. and Panfili, E. (2002). Polyunsaturated fatty acids of germ cell membranes, glutathione and glutathione-dependent enzyme-PHGPx: From basic to clinic. Contraception, 65:301–304.

Lessard, C., Parent, S., Leclerc, P., Bailey, J.L. and Sullivan, R. (2000). Cryopreservation Alters the Levels of the Bull Sperm Surface Protein P25b. Jour. of Andro., 21:700-707.

Liu, Z., Foote, R.H. and Brockett, C.C. (1998). Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology, 37: 219-230.

Matsuoka, T., Imai, H., Kohno, H. and Fukui, Y. (2006). Effects of bovine serum albumine and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa. J.. of Reprod. and Devel., 52:675-683.

Matsuoka, T., Imai, H., Kohno, H. and Fukui, Y. (2006). Effects of bovine serum albumine and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa. J. of Reprod. and Devel., 52:675-683.

Mohanty, D.N. and Ansari, M.R. (2004). Studies on certain seminal plasma enzymes of crossbred bulls in relation to freezability of semen. Ind. J. of Anim. Sci., 25:29-32.

Ohkawa, H., Ohishi, N. and Yagi, K. (1979). Assay of lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95:351–358.

Perumal, P., Barik, A.K., Mohanty, D.N., Das, R.K. and Mishra, P.C. (2009). Seminal characteristics of Jersey crossbred bulls. In Proc. XXVth annual convention of the Indian Society for Study of Animal Reproduction and International Symposium, 196.

Platov, E.M. (1988). Cryopreservation of ram spermatozoa. In: Cryopreservation of spermatozoa of farm animals. Leningrad: Agropromizda, 161–95.

Purdy, P.H. (2006). A review on goat sperm cryopreservation. Small Ruminant Research, 6:215-225.

Rasul, Z., Nasim, A. and Anzar, M. (2001). Changes in motion characteristics, plasma membrane integrity and acrosome morphology during cryopreservation of buffalo spermatozoa. Jour. of Andro., 22:278-283.

Rasul, Z., Nasim, A. and Anzar, M. (2001). Changes in motion characteristics, plasma membrane integrity and acrosome morphology during cryopreservation of buffalo spermatozoa. Jour. of Andro., 22:278-283.

Reda, I., El-Sheshtawy, Gamal, A.S. and El-Nattat, W.S. (2015). Effects of different concentrations of sucrose or trehalose on the postthawing quality of cattle bull semen. Asian Pacific J. of Reprod., 4:26-31.

Reddy, N.S.S. Mohanarao. G.J. and Atreja, S.K. (2010). Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Anim. Repro. Sci., 119:183-190.

Saacke, R.G, Nadir, S., Dalton, J.C., Bame, J.H., DeJarnette, J.M. and Degelos, S. et al. (1994). Accessory sperm evaluation and bull fertility: an update. Proc. 15th Tech. Conf. Artif. Insem. and Reprod. Nat’l. Assoc. Anim. Breeders, Columbia, MO, 57-67.

Schmeil, M., Vázquez, I. and Graham, E. (1986). The effect of non penetrating cryoprotectants added to test–yolk–glycerol extender on the post-thaw motility of ram spermatozoa. Cryobiology, 23:512–517.

Serpil, S., Tuncer, P.B., Bucak, M.N. and Ulutas, P.A. (2009). Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen. Acta Veterinaria Brno, 78:463–469.

Shaikh, K.Q, Nakhashi, H.C., Suthar, B.N, Sutaria, P.T and Sharma, V.K. (2016). Trehalose as semen diluent additive for cryopreservation of Kankrej bull’s semen, Life Sciences Leaflets, 71:29–40.

Sikka, S.C. (1996). Oxidative stress and role of antioxidants in normal and abnormal sperm function. Frontiers in Bioscience. 1:78-86.

Sitaula, R., Elmoazzen, H., Toner, M. and Bhowmick, S. (2009). Desiccation tolerance in bovine sperm: a study of the effect of intracellular sugars and the supplemental roles of an antioxidant and a chelator. Cryobiology, 58:322-330.

Sood, S., Malecki, I.A., Tawang, A. and Martin, A. (2012). A survival of emu (Dromaius novaehollandiae) sperm preserved at subzero temperatures and different cryoprotectant concentrations. Theriogenology, 78:1557-1569.

Squires, E.L., Keith, S.L. and Graham, J.K. (2004). Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology, 62(6):1056-1065.

Steel, R.G.D., Torrie, J.H. and Dicky, D.A. (1997). Principles and Procedures of Statistics, A Biometrical Approach. 3rd Edition, McGraw Hill, Inc. Book Co., New York, 352- 358.

Sztein, J.M., Noble, K., Farley, J.S. and Mobraaten, L.E. (2001). Comparison of permeating and nonpermeating cryoprotectants for mouse sperm cryopreservation. Cryobiology, 42:28-39.

Sztein, J.M., Noble, K., Farley, J.S. and Mobraaten, L.E. (2001). Comparison of permeating and nonpermeating cryoprotectants for mouse sperm cryopreservation. Cryobiology, 42:28-39.

Thananurak, P., Vongpralup, T., Sittikasamkit, C. and Sakwiwatkul, K. (2016). Optimization of Trehalose Concentration in Semen Freezing Extender in Thai Native Chicken Semen, Thai J. of Vet. Medi., 46:287-294.

Tuncer, P.B., Umut, T.D., Serhat, B., Taner, O., Erdem, C.K. and Halil, E., et al. (2013). Effects of different doses of trehalose supplementation in egg yolk extender in frozen–thawed Angora buck semen. Small Rum. Research, 113:383-389.

Upreti, G.C., Jensen, K., Munday, R., Duganzich, D.M., Vishwanath, R. and Smith, J.F. (1998). Studies on aromatic amino acid oxidase activity in ram spermatozoa: Role of pyruvate as an antioxidant. Anim. Reprod. Sci., 51:275-287.

Uysal, O. and Bucak, M.N. (2007). Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Veterinaria Brno, 76:383-90.

Uysal, O., Bucak, N.B. (2009). The role of different trehalose concentrations and cooling rates in freezing of ram semen. Ankara University, Veterinary Faculty Degree, 56:99-103.

Watson, P.F. (2000). The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci., 61:481-492.

Woelders, H., Matthijs, A. and Engel, B. (1997). Effects of trehalose and sucrose, osmolality of the freezing medium and cooling rate on viability and intactness of bull sperm after freezing and thawing. Cryobiology., 35:93-105.

Downloads

Published

2023-11-07

Issue

Vol. 19 No. 6 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov-Dec) 2023

Section

Articles

License

Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kumar, G., Yadav, D.K., Saxena, A., Sachan, V., Kumar, A., Swain, D.K., & Yadav, B. (2023). Effect of Trehalose on Cryopreservation of Hariana Bull Spermatozoa. Indian Journal of Veterinary Sciences and Biotechnology, 19(6), 8–11. https://doi.org/10.48165/ijvsbt.19.6.02
Crossref
Scopus
Google Scholar
Europe PMC