Polymorphisms within IGF1 and IGF1R Candidate genes in Gaolao Cattle Population

Authors

  • Aboli Wankhade Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur 440006, Maharashtra, India
  • Deepak Kale Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur 440006, Maharashtra, India
  • Jaya Singh Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur 440006, Maharashtra, India
  • Dinesh Patil Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur 440006, Maharashtra, India
  • Sachin Bonde Department of Veterinary Biochemistry, Nagpur Veterinary College, MAFSU, Nagpur 440006, Maharashtra, India
  • Atul Dhok Department of Animal Nutrition, Nagpur Veterinary College, MAFSU, Nagpur 440006, Maharashtra, India
  • Krushna Bahiram Department of Veterinary Physiology, Nagpur Veterinary College, MAFSU, Nagpur 440006, Maharashtra, India

DOI:

https://doi.org/10.48165/ijvsbt.19.6.11

Keywords:

IGF1, IGF1R, Milk traits, Polymorphism, Zebu cattle

Abstract

IGF1 gene expresses during lactation, mediates galactopoietic effects, and regulates metabolism and growth and IGF1R gene relation with milk components. The objectives were to identify polymorphisms within IGF1 and IGF1R genes & find associations with milk traits. The blood was collected from 262 purebred cattle from the breeding tract, and test-day milk traits were recorded. PCR- SnaBI analysis of the 5′UTR region of the IGF1 gene revealed only BB genotype; however; IGFG1-SSCP screening of 5′UTR region of IGF1 gene revealed polymorphism with pattern A frequency as 0.685 and of B as 0.314 respectively. IGFG2 -TasI PCR-RFLP analysis of 5′UTR region of IGF1 gene revealed CC genotype. The monomorphic PCR-SSCP followed by sequencing revealed 14 computational SNPs. IGFRG5-TaqI PCR-RFLP within exon-21 region exhibited genetic variation at the locus. The IGFRG5 screening of the same exon 21 regions revealed two polymorphic SSCP patterns, which after direct sequencing revealed SNP A>G at 36th position. The findings will enrich IGF1 and IGFR candidate gene variation status and will aid in selecting and breeding decisions to improve Gaolao cattle.

 

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References

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Published

2023-11-07

Issue

Vol. 19 No. 6 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov-Dec) 2023

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Wankhade, A., Kale, D., Singh, J., Patil, D., Bonde, S., Dhok, A., & Bahiram, K. (2023). Polymorphisms within IGF1 and IGF1R Candidate genes in Gaolao Cattle Population. Indian Journal of Veterinary Sciences and Biotechnology, 19(6), 50–55. https://doi.org/10.48165/ijvsbt.19.6.11
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