Molecular Diagnosis and Haemato-biochemical Alteration in Canine Parvoviral Infection in Dogs

Authors

  • Piyush S Zope Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India
  • Sunil P Waghmare epartment of Veterinary Clinical Complex, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India
  • Kishor s Pajai Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India
  • Dhananjay G Dighe Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India
  • Sunil W Hajare Department of Veterinary Pharmacology and Toxicology, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India
  • Bhupesh P Kamdi Department of Veterinary Pathology, Post Graduate Institute of Veterinary and Animal Sciences, Akola-444104, India

DOI:

https://doi.org/10.48165/ijvsbt.19.5.19

Keywords:

Canine Parvovirus, Haematological-biochemical alterations, PCR

Abstract

The present study was undertaken to diagnose and assess the haematological & biochemical alteration in parvoviral gastroenteritis in dogs. Total of 12 dogs suspected for parvovirus infection were diagnosed by using lateral flow-based immunochromatographic assay rapid canine parvovirus (CPV) antigen test kit and polymerase chain reaction (PCR) technique in the faeces of affected dogs. All 12 dogs were found positive by a rapid antigen test kit and VP2 gene of CPV PCR assay using the CPV-2ab primer pair and expected size of 681 bp.  The haemato-biochemical study revealed a decrease in Hb, TEC, serum total protein, albumin and globulin, whereas the increase in absolute neutrophil count and BUN in parvovirus-infected dogs, indicated anemia and hypoproteinemia in parvovirus infected dogs.

Downloads

Download data is not yet available.

References

Abdullaziz, I, M. Aly, I. Elshahawy (2022). Clinical, haemato-biochemical alterations with acute phase response in canine parvoviral enteritis. Damanhour Journal of Veterinary Sciences,7(1):23-27.

Agnihotri, D., Singh, Y. Maan, S. Jain, V. Kumar, A. Sindhu, N. and Kumar, A. (2017). Molecular detection and clinico-haematological study of viral gastroenteritis in dogs. Haryana Vet, 56(1):72-76.

Amaravathi, M., Kumar, B. Reddy, C. and Jyosthna Devi, S. (2016). Clinico haemato biochemical changes in canine parvoviral infection. International Journal of Advanced Multidisciplinary Research, 3(3):31-33.

Arora, R., Tyagi, A. Shekhar, S. Rajora, V.S. and Arora, N. (2018). Haemato-biochemical alterations in gastroenteritis affected dogs. J Entomol Zool Stud, 6(5): 972-974.

Baruah, MS., Hazarika, GC. and Phukan, A. (2007). Clinicobiochemical profile in Canine parvoviral infection. The Ind. Vet. J. 84(2):212-214.

Berghoff, N. and Steiner, J. M. (2011). Laboratory tests for the diagnosis and management of chronic canine and feline enteropathies. Vet. Clin. Small Anim. 41:311-328.

Bhalekar, P. P. (2020). Evaluation of Bovine Colostrum in Parvo Virus Enteritis in Dogs. M.V.Sc. Thesis submitted to Maharashtra Animal and Fishery Sciences University, Nagpur.

Bhargavi, M., Shobhamani, B. Nalini Kumari, K. and Srilatha, C. (2017). Diagnostic Aspects and Haematobiochemical Changes Associated with Canine Parvoviral Enteritis in Dogs. Int. J. Curr. Microbiol. App. Sci. 6(11): 3357-3364.

Biswas, S., Chakravortya, D. and Pradhan, N. R. (2005). Clinical and Haemato-biochemical changes in parvovirus infection in dogs. Indian J Vet. Med;25(1):16-18.

Boosinger TR, Rebar, AH. DeNicola, DB and Boon, GD. (1982). Bone marrow alterations associated with canine parvoviral enteritis. Vet Pathol, 19(5):558–561.

Brar, R. S., Sandu, H. S and Singh, A. (2004). Veterinary clinical diagnosis by Laboratory methods, Ist Edn, Kalyani publishers, New Delhi.

Burchell, R. K., Schoeman, J. P and Leisewitz, A. L. (2014). The central role of chloride in the metabolic acid-base changes in canine parvoviral enteritis. The Veterinary Journal 200(1):152-156.

Christ-Crain, M., Meier, C. Puder, J. Staub, J. J. Huber, P. R. Keller, U and Müller, B. (2004). Changes in liver function correlate with the improvement of lipid profile after restoration of euthyroidism in patients with subclinical hypothyroidism. EXCLI J. 3:1-9.

Crawford, P. C and Sellon, R. K. (2010). Canine Viral Diseases. In: Text book of Veterinary Internal Medicine. 7th ed.:958 -960.

Decaro, N. and Buonavoglia, C. (2012). Canine parvovirus—a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Veterinary Microbiology, 155(1):1-12.

Decaro, N., Desario, C. Billi, M. Lorusso, E. Colaianni, M. L. Colao, V. and Buonavoglia, C. (2013). Evaluation of an in-clinic assay for the diagnosis of canine parvovirus. The Veterinary Journal, 198(2), 504-507.

Dongre, J., Mehta, H.K. Maheshwari, P. and Mehta, M. (2013). Haematological changes in dogs affected with Canine parvovirus infection. In: 31st Annual Convention of Indian Society for Veterinary Medicine and National Symposium on “Advancing veterinary medicine and its specialities for augmented productivity and health: issues and strategies in farm and companion dogs”, NDVSU, Mhow, 59.

Dorlikar, P. R., Warke, S. R. Tumlam, U. M. Ingle V. C. (2018). Molecular characterization of Canine parvovirus-2 infection in and around Nagpur. M.V.Sc. Thesis submitted to Maharashtra Animal and Fishery and Science University.

Elsayed, N. M., Kubesy, A. A. and Salem N. Y. (2020). Altered blood oxidative stress biomarkers in association with canine parvovirus enteritis. Comparative Clinical Pathology, 29:355-359.

Goddard, A. and Leisewitz A. L. (2010). Canine parvovirus. Veterinary Clinics: Small Animal Practice, 40(6), 1041-1053.

Grigonis, A., Macijauskas, V. and Zamokas G. (2002). Examination of liver functions in dogs with parvovirus enteritis. Veterinarian-ir-Zootechnika; 17:23-28.

Harizan, M., Khinchi, R. K. Sharma, S. K. and Meena (2021). Alterations in haemato-biochemical and oxidative stress indices in dogs affected with parvoviral enteritis. The Pharma Innovation Journal;10(8S):428-431.

Jacob, RM., Weiser, MG. and Kowalski JJ. (1980). Clinic pathogenic features of canine parvoviral enteritis. Journal of American Veterinary Medicine Association;16:809-813.

Kataria, D., Agnihotri, D. Jain, V. Charaya, G. and Singh Y. (2020). Molecular occurrence and therapeutic management of canine parvovirus infection in dogs. Int. J. Curr. Microbiol. Appl. Sci, 9:1770-1779.

Khare, D. S., Gupta, D. K. Shukla, P. C. Das, G. Meena, N. S. and Khare R. (2020). Clinical and haemato-biochemical changes in canine parvovirus infection. Journal of Pharmacognosy and Phytochemistry, 9(4):1601-1604.

Khadse, M. B., Rambhau, S. and Kolangath S. (2023). Molecular Detection and Phylogenetic Analysis of Canine Parvovirus-2 in Dogs. Indian Journal of Veterinary Sciences & Biotechnology, 19(2):54-57.

Kramer, J. M., PC, M., RVH, P. (1980). Canine parvovirus: update. Vet. Med. Small Animal Clin.;75(10):1541-55

Kubesy, A. A., Rakha, G. M., Salem, A. H., and Jaheen (2019). Altered blood procalcitonin, C-reactive protein, and leucocytes count in association with canine parvovirus (CPV) enteritis. Comparative Clinical Pathology, 28:1095-1099.

Kumar, R., Kumar, B., Kumar, S., Singh, M. K., and Kumar, R. (2020). Study of biochemical parameter in canine parvo virus infected canines. Pharma Innovation J, 9, 40-43.

Macintire, DK and Smith-Carr, S. (1997). Canine parvovirus. Part II. Clinical signs, diagnosis and treatment. Comp. Contin. Edu. Prac. Vet., 19(3): 291-302.

Mazzaferro, E. M. (2020). Update on canine parvoviral enteritis. Veterinary Clinics: Small Animal Practice, 50(6):1307-1325.

Mohan, R., Nauriyal, D. C., and Singh, K. B. (1991). Haematological and biochemical alterations in canine parvo virus infection. Indian Journal of Veterinary Medicine. 11(1 & 2):52-53.

Navarro, C. (2020). Detection of canine parvovirus in dogs by means polymerase chain reaction. Am J Biomed Sci Res, 7(10.34297)

Oser, B.L. (1986). Hawk’s Physiological chemistry.14th ed. Tata McGraw Hill Publishing Co. Ltd, New Delhiber

Patel Hemali, Rao, Neha. Patel, Nitin R. and Gohil, Nilesh. (2022). Haemato-biochemical changes and therapeutic efficacy of adjuvant therapy on canine parvovirus gastroenteritis. The Pharma Innovation Journal. 11(11S):2314-2320

Pandya, M. S., Sharma, K. K., Kalyani, H. I. and S. P. Sakhare (2017). Study on host predisposing factors and diagnostic tests for canine parvovirus (CPV-2) infection in dogs. Journal of Animal Research, 7(5):897-902.

Pereira, C. A., Monezi, T. A., Mehnert, D. U., D’Angelo, M., and Durigon, E. L. (2000). Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay. Veterinary microbiology, 75(2), 127-133.

Prittie J. (2004). Canine parvoviral enteritis: a review of diagnosis, management, and prevention. Journal of Veterinary Emergency and Critical Care 14(3) 2004, pp167-176

Rai, A., Nauriyal, DC. and Mohan, R. (1994). A note on haematological observations in canine parvoviral gastroenteritis. Indian Veterinary Journal; 13:99.

Ramprabhu, R., Prathaban, S. Nambi, A. P. and Dhanapalan, P. (2002). Haemorrhagic gastroenteritis in dogs- a clinical profile Indian Vet. J. 79:374-376.

Sagar, A., Roy, S. and Roy, M. (2008). Clinico, haemato-bochemical changes and diagnosis of canine parvoviral enteritis. Intas Polivet. 9:262-265.

Sambrook, J. and Russell D. W. (2006). Alkaline agarose gel electrophoresis. CSH Protoc, (1), 2006.

Saravanan, S., Ramprabhu, R. Thangaraj, Mohanapriya & T, Chitra, K.K., Ponnu Swamy (2020). Retrospective study on risk factors and haemato- biochemical alterations associated with canine parvo viral enteritis. Journal of Entomology and Zoology Studies. 8:342 - 346.

Shah, S. A., Sood, N. K. Wani, N. Gupta, K. and Singh, A. (2013). Haemato-biochemical changes in canine parvoviral infection. Indian J. Vet. Pathol., 37(2):131-133.

Sheikh, M. O., Rashid, P. M. A. Marouf, A. S. Raheem, Z. H. Manjunath, S. and Janga, S. C. (2017). Molecular typing of canine parvovirus from Sulaimani, Iraq and Phylogenetic analysis using partial VP2 gene. Bulgarian Journal of Veterinary Medicine, 20(3):225–235

Singh, M., Manikandan, R. De, U. K. Chander, V. Paul, B. R. Ramakrishnan, S. and Maramreddy, D. (2022). Canine Parvovirus-2: An emerging threat to young pets. Recent Advances in Canine Medicine, in press.

Tanwar J., Choudhary S., and Bihani, G. J. DK. (2020). Comparative study for efficacy of Immunochromatography antigen assay (ICA) test kit with Polymerase chain reaction (PCR) to detect canine parvovirus. Journal of Entamology and Zoology, 8(4):416-419.

Thakur M. and Thakur, R. (2018). Haemato-biochemical alterations in canine Parvo Virus infection affected pups. Himachal Journal of Agricultural Research, 43(2):131-134.

Tilley, L. P., and Smith, W. K. (2016). Blackwell’s five minute veterinary consult: canine and feline. John wiley and sons, Oxford, UK.(6):1416.

Tinky, S.S., Ambily, S., Nair, S. R. and Mini, M. (2015). Utility of a rapid immunochromatographic strip test in detecting canine parvovirus infection compared with polymerase chain reaction. Veterinary World, 8(4):523.

Van den Berg, M.F., Schoeman, J.P., Defauw, P. Whitehead, Z. Breemersch, A. Goethals, K. Daminet, S. and Meyer, E. (2018). Assessment of acute kidney injury in canine parvovirus infection: Comparison of kidney injury biomarkers with routine renal functional parameters. Vet J, 242:8-14.

Vakili, N., Mosallanejad, B., Avizeh, R.S., Shapouri, M.R. and Pourmahdi (2014). A comparison between PCR and Immunochromatography assay (ICA) in diagnosis of hemorrhagic gastroenteritis caused by Canine parvovirus. Arch. Razi Inst., 69(1):27-33

Yadav, Gupta, S. R. and Sharma, C. S. (2011). Clinical haematology in dogs affected with haemorrhagic gastroenteritis. Vet. Pract;12(1):60-62.

Published

2023-09-10

How to Cite

Zope, P.S., Waghmare, S.P., Pajai, K. s, Dighe, D.G., Hajare, S.W., & Kamdi, B.P. (2023). Molecular Diagnosis and Haemato-biochemical Alteration in Canine Parvoviral Infection in Dogs. Indian Journal of Veterinary Sciences and Biotechnology, 19(5), 93–96. https://doi.org/10.48165/ijvsbt.19.5.19