Virulence Characterization of Faecal Escherichia coli Isolates from Wild Animals

Authors

  • Kamlesh Kumar Department of Veterinary Microbiology, College of Veterinary Science & A.H., Anjora, DauShriVasudevChandrakarKamdhenuVishwavidyalaya, Durg, Chhattisgarh, India
  • Chandrahas Sannat Department of Veterinary Microbiology, College of Veterinary Science & A.H., Anjora, DauShriVasudevChandrakarKamdhenuVishwavidyalaya, Durg, Chhattisgarh, India
  • S.D.Hirpurkar Department of Veterinary Microbiology, College of Veterinary Science & A.H., Anjora, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, India
  • Nidhi Rawat Department of Veterinary Microbiology, College of Veterinary Science & A.H., Anjora, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, India
  • Jasmeet Singh Department of wild animal health and forensic, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, India
  • Savita Sahu Department of Veterinary Pathology, College of Veterinary Science & A.H., Anjora, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, India
  • Rajat Ratnayke Department of Veterinary Microbiology, College of Veterinary Science & A.H., Anjora, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, India
  • Vishvajeet Dilliwar Department of Veterinary Microbiology, College of Veterinary Science & A.H., Anjora, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, India

DOI:

https://doi.org/10.48165/ijvsbt.19.5.18

Keywords:

Wild animals, Feacal E.coli, Haemagglutination, Gelatinase, fimH

Abstract

The present study was undertaken to characterize virulence factors associated with fecal Escherichia coli (E.coli) isolates (n=22) from wild animals. Isolates were phenotypically characterized for haemolytic, haemagglutination and gelatinase activity whereas presence of adhesion genes viz. fimH and papG1 was detected genotypically by PCR. Further, whole blood survival assay of E. coli isolates (n=6) was performed using human and goat blood. Isolates exhibited heamolytic (45.45%) as well as gelatinase activity (27.27%) and led mannose sensitive agglutination of human (45.45%), dog (40.90%) and chicken (27.27%) RBC.  fimH gene was detected in 27.27% of the isolates whereas none of the isolates was found positive for PapG1 gene. Isolates survived, got adapted and then grew successfully in human as well as goat blood. It can be concluded from present study that wild animals are potent reservoir of virulent E. coli that could adversely affects in-contact human and livestock population.

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References

Al-Yassari, A. K. S., Alomairi, J. G., & Atiyah, S. A. A. (2020). Comparative study for the frequency of papC, fimH and iucD genes in E. coli isolates from UTI and vaginal infections. International Journal of Pharmaceutical Research, 12, 4563-4567.

Baby, S., Karnaker, V. K., & Geetha, R. K. (2014). Serum Bactericidal Resistance in Uropathogenic E. coli. International Journal of Current Microbiology and Applied Sciences, 3, 823-828.

Chaturvedi, A., Gautam, A., Shukla, S., & Singh, V. K. (2016). Determination of virulence factors of Escherichia coli isolated from urinary tract infection patients. The Pharma Innovation, 3(8), 29-32.

Desai, S., Kagal, R. A., & Bharadwaj, R. (2013). Virulence factors in uropathogenic Escherichia coli (UPEC) causing urinary tract infections. International Journal of Applied and Basic Medical Research, 2, 886-896.

Flores-Mireles, A.L., Walker, J.N., Caparon, M., & Hultgren, S.J. (2015). Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nature Reviews Microbiology, 13(5), 269-84.

Guenther, S., Ewers, C., & Wieler, L.H. (2011). Extended-spectrum beta-lactamases producing E. coli in wildlife, yet another form of environmental pollution? Frontiers in Microbiology, 2, 246.

Jadhav, S., Hussain, A., Devi, S., Kumar, A., Parveen, S., Gandham, N., Wieler, L.H., Ewers, C., & Ahmed, N. (2011). Virulence characteristics and genetic affinities of multiple drug resistant uropathogenic Escherichia coli from a semi urban locality in India. PloS one, 6, e18063.

Johnsen, N., Hamilton, A.D.M., Greve, A.S., Christensen, M.G., Therkildsen, J.R., Wehmöller, J., Skals, M., & Praetorius, H.A. (2019). α-Haemolysin production, as a single factor, causes fulminant sepsis in a model of Escherichia coli-induced bacteraemia. Cell Microbiology, 6, e13017.

Kudinha, T., & Kong, F. (2022). Distribution of papG alleles among uropathogenic Escherichia coli from reproductive age women. Journal of Biomedical Science, 29(1), 66.

Lane, M.C., Simms, A.N., & Mobley, H.L. (2007). complex interplay between type 1 fimbrial expression and flagellum-mediated motility of uropathogenic Escherichia coli. Journal of Bacteriology, 189(15), 5523-33.

Luna-Pineda, V.M., Ochoa, S.A., Cruz-Córdova, A., Cázares-Domínguez, V., Reyes-Grajeda, J.P., Flores-Oropeza, M.A., Arellano-Galindo, J., Castro-Hernández, R., Flores-Encarnación, M., Ramírez-Vargas, A., Flores-García, H.J., Moreno-Fierros, L., & Xicohtencatl-Cortes, J. (2018). Features of urinary Escherichia coli isolated from children with complicated and uncomplicated urinary tract infections in Mexico. PLoS One, 13, e0204934.

Melican, K., Sandoval, R.M., Kader, A., Josefsson, L., Tanner, G.A., Molitoris, B.A., & Richter-Dahlfors, A. (2011). Uropathogenic Escherichia coli P and Type 1 fimbriae act in synergy in a living host to facilitate renal colonization leading to nephron obstruction. PLoS Pathogens, 7, e1001298.

Mittal, S., Sharma, M., & Chaudhary, U. (2014). Study of virulence factors of uropathogenic Escherichia coli and its antibiotic susceptibility pattern. Indian Journal of Pathology and Microbiology, 57(1), 61-64.

Momtaz, H., Karimian, A., Madani, M., SafarpoorDehkordi, F., Ranjbar, R., Sarshar, M., & Souod, N. (2013). Uropathogenic Escherichia coli in Iran: serogroup distributions, virulence factors and antimicrobial resistance properties. Annals of Clinical Microbiology and Antimicrobials, 12, 8.

Nam, E. H., Ko, S., Chae, J. S., & Hwang, C. Y. (2013). Characterization and zoonotic potential of uropathogenic Escherichia coli isolated from dogs. Journal of Microbiology and Biotechnology, 23, 422-429.

Sande, C., Bouwman, C., Kell, E., Nickerson, N.N., Kapadia, S.B., & Whitfield, C. (2019). Structural and Functional Variation in Outer Membrane Polysaccharide Export (OPX) Proteins from the Two Major Capsule Assembly Pathways Present in Escherichia coli. Journal of Bacteriology, 201(14), e00213-19.

Sannat, C. (2021). Studies on efficacy of Hemidesmus indicus root extract and antibiotics against biofilm forming methicillin resistant Staphylococcus aureus (MRSA). Ph.D.Thesis. DSVCKV, Anjora, Durg, Chhattisgarh.

Sannat, C., Hirpurkar, S.D., Shakya, S., Jain, T., Tripathi, S.M., Chandrakar, C., Patyal, A., Rawat, N., & Gupta, A.K. (2022). Cost Effective Screening and Characterization of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Bovine Mastitis in India. Journal of Animal Research, 12(03), 343-349

Sauer, M.M., Jakob, R.P., Luber, T., Canonica, F., Navarra, G., Ernst, B., Unverzagt, C., Maier, T., & Glockshuber, R. (2019). Binding of the Bacterial Adhesin FimH to Its Natural, Multivalent High-Mannose Type Glycan Targets. Journal of American Chemical Society, 141(2), 936-944.

Shah, C., Baral, R., Bartaula, B., & Shrestha, L. B. (2019). Virulence factors of uropathogenic Escherichia coli (UPEC) and correlation with antimicrobial resistance. BMC microbiology, 19(1), 204.

Shruthi, N., Kumar, R., & Kumar, R. (2012). Phenotypic study of virulence factors in Escherichia coli isolated from antenatal cases, catheterized patients, and faecal flora. Journal of Clinical and Diagnostic Research, 6(10), 1699-1703.

Tabasi, M., Karam, M. R. A., Habibi, M., Yekaninejad, M. S., & Bouzari, S. (2015). Phenotypic assays to determine virulence factors of uropathogenic Escherichia coli (UPEC) isolates and their correlation with antibiotic resistance pattern. Osong Public Health and Research Perspective, 6, 261-268.

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Published

2023-09-10

Issue

Vol. 19 No. 5 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Sep-Oct) 2023

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kumar, K., Sannat, C., Hirpurkar, S.D., Rawat, N., Singh, J., Sahu, S., … Dilliwar, V. (2023). Virulence Characterization of Faecal Escherichia coli Isolates from Wild Animals. Indian Journal of Veterinary Sciences and Biotechnology, 19(5), 88–92. https://doi.org/10.48165/ijvsbt.19.5.18
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