Clinical, Haemato-biochemical Evaluation and Comparison of Polymerase chain reaction with Conventional Blood Smear Examination for the Detection of Babesia gibsoni Infection in Dogs

Authors

  • Peddineni Sree Krishna Sai Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India
  • P Vijayalakshmi Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India
  • @ Prabavathy A Abiramy Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India
  • N Devadevi Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India
  • K Rajkumar Department of Veterinary Clinical Complex, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India
  • D Himaja Satya Sai ratnam Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India

DOI:

https://doi.org/10.48165/ijvsbt.19.5.06

Keywords:

Babesia gibsoni, PCR, blood smear examination, dogs

Abstract

The objective of the present study was to detect Babesia gibsoni infection in dogs which are presented to Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry. A total of 85 dogs with clinical signs such as inappetence, fever, enlarged lymph nodes and tick infestation were screened. The diagnostic efficacy of polymerase chain reaction (PCR) technique has been compared with blood smear examination to detect B. gibsoni infection in dogs. By   blood smear and PCR examination, six (7.1%) dogs and 28 (42.4%) dogs were positive for B. gibsoni infection respectively. Clinical examination of dogs with B. gibsoni showed tick infestation, anorexia, dullness, pyrexia, pale/congested conjunctival mucus membrane, tachypnoea and swollen lymph nodes. In addition, few dogs had icterus and haemoglobinuria. Laboratory examination of blood samples of B. gibsoni dogs revealed significant reduction in haemoglobin, PCV, erythrocyte count, platelet count and serum albumin levels. Significant increase in levels of ALP, globulin, bilirubin, BUN and creatinine were recorded.

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Author Biographies

  • P Vijayalakshmi , Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India

    Professor and Head, Dept. of Veterinary Medicine

  • @ Prabavathy A Abiramy , Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India

    Professor, Dept. of Veterinary Medicine

  • N Devadevi , Department of Veterinary Medicine, Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605009, India

    Assistant Professor, Dept. of Veterinary Medicine

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Published

2023-09-10

Issue

Vol. 19 No. 5 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Sep-Oct) 2023

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Sai, P.S.K., Vijayalakshmi , P., Abiramy , @ P.A., Devadevi , N., Rajkumar, K., & ratnam, D.H.S.S. (2023). Clinical, Haemato-biochemical Evaluation and Comparison of Polymerase chain reaction with Conventional Blood Smear Examination for the Detection of Babesia gibsoni Infection in Dogs. Indian Journal of Veterinary Sciences and Biotechnology, 19(5), 33–36. https://doi.org/10.48165/ijvsbt.19.5.06
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