In-Vitro Neutralization Efficacy of Taxifolin against  Anthrax Toxins

Authors

  • Shumaila Taskeen Department of Veterinary Public Health, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly-243122, Uttar Pradesh, India
  • Deepak B Rawool Department of Veterinary Public Health, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly-243122, Uttar Pradesh, India
  • Somya Aggarwal School of Biotechnology, Jawahar Lal Nehru University, New Delhi-110067, India
  • Vikas Somani School of Biotechnology, Jawahar Lal Nehru University, New Delhi-110067, India
  • Rakesh Bhatnagar School of Biotechnology, Jawahar Lal Nehru University, New Delhi-110067, India

DOI:

https://doi.org/10.48165/ijvsbt.20.1.10

Keywords:

Anthrax, Lethal toxin, Edema toxin, Protective antigen, Taxifolin

Abstract

The present study was undertaken to evaluate the protective efficacy of Taxifolin against anthrax toxins viz., lethal toxin (LT) and edema toxin (ET) in in-vitro model. The MTT assay and cAMP ELISA was deployed to estimate the LT induced cytoxicity and ET induced intracellular increase in cyclic AMP respectively, wherein 10μg PA +1μg LF/EF was deduced as the cytotoxic dose. The assays revealed an anticipated dose dependent decrease in cytotoxicity at varying concentrations (100μM to 0.1μM) of taxifolin when challenged against optimized cytotoxic dose of LT (10μg PA +1μg LF). Similar dose dependent decrease in intracellular cAMP levels was observed at all the evaluated concentrations of taxifolin (100μM to 0.1μM) against cytotoxic dose of ET (10μg PA +1μg EF). The neutralization potential of taxifolin against both the anthrax toxins pave a way for future prospects of experimentation using combination of taxifolin with other such inhibitors and/or antibiotics in in vitro and in vivo model, which could therefore be helpful in formulating therapeutics against anthrax.

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References

Abugri, D. A., Witola, W. H., Russell, A. E., & Troy, R. M. (2018). In vitro activity of the interaction between taxifolin (dihydroquercetin) and pyrimethamine against Toxoplasma gondii. Chemical Biology & Drug Design, 91(1), 194-201.

Antonelli, A. C., Zhang, Y., Golub, L. M., Johnson, F., & Simon, S. R. (2014). Inhibition of anthrax lethal factor by curcumin and chemically modified curcumin derivatives. Journal of enzyme inhibition and medicinal chemistry, 29(5), 663-669.

Artenstein, A. W., Opal, S. M., Cristofaro, P., Palardy, J. E., Parejo, N. A., Green, M. D., & Jhung, J. W. (2004). Chloroquine enhances survival in Bacillus anthracis intoxication. Journal of Infectious Diseases, 190(9), 1655-1660.

Bower, W. A., Hendricks, K. A., Vieira, A. R., Traxler, R. M., Weiner, Z., Lynfield, R., & Hoffmaster, A. (2022). What Is Anthrax?. Pathogens, 11(6), 690.

Cote, C. K., & Welkos, S. L. (2015). Anthrax toxins in context of Bacillus anthracis spores and spore germination. Toxins, 7(8), 3167-3178.

Dell'Aica, I., Dona, M., Tonello, F., Piris, A., Mock, M., Montecucco, C., & Garbisa, S. (2004). Potent inhibitors of anthrax lethal factor from green tea. EMBO reports, 5(4), 418-422.

Dumetz, Fabien, Grégory Jouvion, Huot Khun, Ian Justin Glomski, Jean-Philippe Corre, Clémence Rougeaux, Wei-Jen Tang, Michèle Mock, Michel Huerre, and Pierre Louis Goossens. "Noninvasive imaging technologies reveal edema toxin as a key virulence factor in anthrax." The American journal of pathology 178, no. 6 (2011): 2523-2535.

Hanna P. C., Acosta D. and Collier R. J. (1993) On the role of macrophages in anthrax. Proc. Natl. Acad. Sci. USA 90: 10198–10201

Kozhikkadan Davis, C., Nasla, K., Anjana, A. K., & Rajanikant, G. K. (2018). Taxifolin as dual inhibitor of Mtb DNA gyrase and isoleucyl-tRNA synthetase: In silico molecular docking, dynamics simulation and in vitro assays. In Silico Pharmacology, 6, 1-11.

Levinsohn, Jonathan L., Zachary L. Newman, Kristina A. Hellmich, Rasem Fattah, Matthew A. Getz, Shihui Liu, Inka Sastalla, Stephen H. Leppla, and Mahtab Moayeri. "Anthrax lethal factor cleavage of Nlrp1 is required for activation of the inflammasome." PLoS pathogens 8, no. 3 (2012): e1002638.

Polyak, Stephen J., Chihiro Morishima, Volker Lohmann, Sampa Pal, David YW Lee, Yanze Liu, Tyler N. Graf, and Nicholas H. Oberlies. "Identification of hepatoprotective flavonolignans from silymarin." Proceedings of the national academy of sciences 107, no. 13 (2010): 5995-5999.

Rhee, M. H., Endale, M., Kamruzzaman, S. M., Lee, W. M., Park, H. J., Yoo, M. J., & Cho, J. Y. (2008). Taxifolin inhibited the nitric oxide production and expression of pro-inflammatory cytokine mRNA in lipopolysaccharide-stimulated RAW264.7 cells. Journal of Experimental & Biomedical Sciences, 14, 147–155.

Tapas, A. R., Sakarkar, D. M., & Kakde, R. B. (2008). Flavonoids as nutraceuticals: a review. Tropical journal of Pharmaceutical research, 7(3), 1089-1099.

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Published

2023-12-22

Issue

Vol. 20 No. 1 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan-Feb ) 2024

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Taskeen, S., Rawool, D.B., Aggarwal, S., Somani, V., & Bhatnagar, R. (2023). In-Vitro Neutralization Efficacy of Taxifolin against  Anthrax Toxins. Indian Journal of Veterinary Sciences and Biotechnology, 20(1), 48–51. https://doi.org/10.48165/ijvsbt.20.1.10
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