Effect of Different Concentrations of Trehalose on Post-Thaw Quality of Surti Buffalo (Bubalus bubalis) Bull Semen

Authors

  • Naveen Kumar Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, RAJUVAS, Nawania 313601, Udaipur, Rajasthan, India.
  • Mitesh Gaur Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, RAJUVAS, Nawania 313601, Udaipur, Rajasthan, India.
  • Dinesh Jhamb Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, RAJUVAS, Nawania 313601, Udaipur, Rajasthan, India.
  • Jayesh Vyas Department of Animal Genetics and Breeding, College of Veterinary and Animal Science, RAJUVAS, Bikaner, 334001 Rajasthan, India.

DOI:

https://doi.org/10.48165/ijvsbt.19.3.15

Keywords:

Cryopreservation, Extender, Semen, Surti bull, Trehalose

Abstract

The present study was conducted to investigate the effects of supplementation of trehalose as an additive in Tris-Fructose-Egg Yolk-Citrate extender on post-thaw semen quality in 24 ejaculates of six Surti buffalo bulls. Semen extended @ 80 million sperm /ml was divided into four equal parts and served as three treatment group as T1 (50 mM trehalose), T2 (100 mM trehalose) and T3 (150 mM trehalose) and one as control (T0). The percentage of post-thaw sperm progressive motility, sperm viability, HOS responsive sperm were higher, whereas sperm abnormalities were lower in treatment T1, T2 and T3 as compared to control. The treatment T2 (100 mM trehalose) had significantly higher post-thaw progressive motility, sperm viability, HOS response sperm percentage and reduced sperm abnormalities as compared to control and other levels of trehalose. In conclusion, supplementation of trehalose at 100 mM concentration significantly (p<0.05) improved freeze-thawed semen quality of buffalo bulls in comparison to control and trehalose concentration of 50 mM and 150 mM in Tris extender. 

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Published

2023-05-09

Issue

Vol. 19 No. 3 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (May- Jun) 2023

Section

Articles

License

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kumar, N., Gaur, M., Jhamb, D., & Vyas, J. (2023). Effect of Different Concentrations of Trehalose on Post-Thaw Quality of Surti Buffalo (Bubalus bubalis) Bull Semen. Indian Journal of Veterinary Sciences and Biotechnology, 19(3), 70–73. https://doi.org/10.48165/ijvsbt.19.3.15
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