Effect of Different Concentrations of Trehalose on Post-Thaw Quality of Surti Buffalo (Bubalus bubalis) Bull Semen
DOI:
https://doi.org/10.48165/ijvsbt.19.3.15Keywords:
Cryopreservation, Extender, Semen, Surti bull, TrehaloseAbstract
The present study was conducted to investigate the effects of supplementation of trehalose as an additive in Tris-Fructose-Egg Yolk-Citrate extender on post-thaw semen quality in 24 ejaculates of six Surti buffalo bulls. Semen extended @ 80 million sperm /ml was divided into four equal parts and served as three treatment group as T1 (50 mM trehalose), T2 (100 mM trehalose) and T3 (150 mM trehalose) and one as control (T0). The percentage of post-thaw sperm progressive motility, sperm viability, HOS responsive sperm were higher, whereas sperm abnormalities were lower in treatment T1, T2 and T3 as compared to control. The treatment T2 (100 mM trehalose) had significantly higher post-thaw progressive motility, sperm viability, HOS response sperm percentage and reduced sperm abnormalities as compared to control and other levels of trehalose. In conclusion, supplementation of trehalose at 100 mM concentration significantly (p<0.05) improved freeze-thawed semen quality of buffalo bulls in comparison to control and trehalose concentration of 50 mM and 150 mM in Tris extender.
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Aboagla, E.M.E., & Terada, T. (2003). Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biology of Reproduction, 69, 1245-1250.
Aisen, E.G., Mesina, V.H., & Ventruino, A. (2002). Cryppreservation and post-thaw fertility of ram semen frozen in different trehalose concentration. Theriogenology, 57, 1801-1808.
Al-Badrany, Q.M., Al-Badryē, K.I., Ibrahim, F.F., & Lateef, W.Y. (2017). Effect of trehalose and steps of freezing on sperm properties of bull frozen in liquid nitrogen. International Journal of Advanced Research in Biological Sciences, 4(4), 189-200.
Anzar, M., & Graham, E.F. (1995). Effect of filtration on post-thaw quality of bull semen. Theriogenology, 43(2), 439-449.
Badr, M.R., Abd El-Malak, M.G., & Hassan, H.M. (2010). Effect of trehalose on cryopreservation, oxidative stress and DNA integrity of buffalo spermatozoa. Journal of Reproduction and Infertility, 1(2), 50-57.
Bittencourt, R.F., Oba, E., De Almeida Biscardea, C.E., Azevedoc, H.C., Bittencourt, M.V., De Menezesa, G.F.O., Da Silva Limaa, A., Da Mata Fuchsa, K., & De LisboaRibeiro Filho, A. (2018). Dimethylacetamide and trehalose for ram semen cryopreservation. Cryobiology, 130, 1-6.
Bucak, M.N., Atessahin, A., Varısili, O., Yuce, A., Tekin, N., & Akcay, A. (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after the freeze–thawing process. Theriogenology, 67, 1060-1067.
Bucak, M.N., Keskin, N., Taşpınar, M., Çoyan, K., Başpınar, N., Cenariu, M.C., & Kurşunlu, A.N. (2013). Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters. Cryobiology, 67(1), 34-39.
Chhillar, S., Singh, V.K., Kumar, R., & Atreja, S.K. (2012). Effects of taurine or trehalose supplementation on functional competence of cryopreserved Karan Fries semen. Animal Reproduction Science, 135, 1-7.
El-Badry, D.A., El-Maaty, A.M. A., & El Sisy, G.A. (2017). The Effect of trehalose supplementation of INRA-82 extender on quality and fertility of cooled and frozen-thawed stallion spermatozoa. Journal of Equine Veterinary Science, 48, 86-92.
El-Sheshtawy, R.I., Sisy, G.A., & El-Nattat, W.S. (2015). Effects of different concentrations of sucrose or trehalose on the post thawing quality of cattle bull semen. Asian Pacific Journal of Reproduction, 4(1), 26-31.
Ghallab, A.M., Shahat, A.M., Fadi, A.M., Ayoub, M.M., & Moawad, A.R. (2017). Impact of supplementation of semen extender with antioxidants on the quality of chilled or cryopreserved Arabian stallion spermatozoa.Cryobiology, 79, 17-20.
Holt, W.V. (2000). Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenology, 53(1), 47-58.
Hu, J.H., Zan, L.S., Zhao, X.L., Li, Q.W., Jiang, Z.L, Li, Y.K. & Li, X. (2010). Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen. Journal of Animal Science, 88, 1657-62.
Jhamb, D. (2021). Effect of L-arginine and trehalose supplementation to semen extender on quality and fertility of cryopreserved stallion semen. Ph.D. Thesis. RAJUVAS, Bikaner, Rajasthan, India.
Medeiros, C.M.O., Forell, F., Oliveira, A.T.D. & Rodrigues, J.L. (2002). Current status of sperm cryopreservation: why isn't it better? Theriogenology, 57(1), 327-344.
Morris, G.J., Faszer, K., Green, J.E., Draper, D., Grout, B.W.W., & Fonseca, F. (2007). Rapidly cooled horse spermatozoa: loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation. Theriogenology, 68(5), 804-812
Reddy, N.S.S., Mohanarao, G.J., & Atreja, S.K. (2010). Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (Bubalus bubalis) sperm quality following cryopreservation. Animal Reproduction Science, 119, 183-190.
Sanocka, D., & Kurpisz, M. (2004). Reactive oxygen species and sperm cells. Reproductive Biology and Endocrinology, 2(1), 1-7.
Snedecor, G.W., and Cochran, W.G. (1994). Statistical Methods, 8thEdn., The lowa State College Press, Inc. USA. 950p.
Tuncer, P.B., Taşdemir, U., Büyükleblebici, S., Özgürtaş, T., Coşkun, E., Erol, H., & Gürcan, İ. S. (2013). Effects of different doses of trehalose supplementation in egg yolk extender in frozen–thawed Angora buck semen. Small Ruminant Research, 113(2-3), 383-389.
Watson, P.F. (2000). The cause of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60(1), 481-492.
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