Occurrence, characterization and antimicrobial resistance pattern of pathogenic Salmonella isolated from chicken and chicken meat products
DOI:
https://doi.org/10.48165/ijvsbt.19.4.06Keywords:
Antibiotic resistance, Chicken meat products, invA, S. TyphimuriumAbstract
The objective of this investigation was to detect the occurrence, characterization, and antimicrobial resistance patterns of pathogenic Salmonella isolated from samples (400) comprising faecal and cloacal samples, environmental samples, chicken meat, and restaurant meat products collected at Udgir, Maharashtra. Following primary isolation and biochemical tests, two isolates were found to be positive for Salmonella spp. and were subjected to in vitro pathogenicity tests. Salmonella genus identification was accomplished by targeting the genus-specific invA gene, and serotype identification was executed by targeting the spy and sdfI genes with the Polymerase Chain Reaction (PCR) technique. The results showed that both isolates were positive for the S. Typhimurium spy gene, indicating a 0.50% overall prevalence of pathogenic Salmonella Typhimurium. Similarly, Both S. Typhimurium isolates tested positive on virulent marker (invA) based PCR, as well as pathogenic potential on Congo red dye binding assay and haemolysis on 5% sheep blood agar, indicating the efficient use of a targeted gene, invA, for early determination of pathogenic potential of Salmonella isolates. An antibiogram study using 15 antibiotics revealed remarkably high resistance to tetracycline, sulphadiazine, nalidixic acid, and ampicillin, suggesting a spike in multidrug resistance in food-borne pathogens to the most commonly used drugs in animal and human medicine.
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Afshari, A., Baratpour A., Khanzade S. and Jamshidi A. (2018). Salmonella enteritidis and Salmonella typhimurium identification in poultry carcasses. Iran. J. Microbiol., 10(1): 45-50.
Agarwal, R.K., Bhilegaonkar K.N., Singh D.K., Kumar A. and Rathore R.S. (2003). Laboratory manual for the isolation and identification of foodborne pathogens. Indian Veterinary Research Institute, Izatnagar, Bareilly, India, pp 35-37.
Barrow, P.A., Jones M.A., Smith A.L. and Wigley P. (2012). The long view: Salmonella-the last forty years. Avian pathol. J. W.V.P.A, 41(5): 413-420.
Barua, H., Biswas P.K., Olsen K.E., Shil S.K. and Christensen J.P. (2013). Molecular characterization of motile serovars of Salmonella enterica from breeder and commercial broiler poultry farms in Bangladesh. PloS one, 8(3): e57811.
Bauer A.W., Kirby M.N., Sherris J.C. and Truck M. (1966). Antibiotic susceptibility testing by standardized single disc method. Am. J. Clin. Pathol. 45: 443-446.
Bergey’s Manual of Systemic Bacteriology (1984). Williams and Wilkins, Baltimore, USA, pp. 543-548.
Candrian, U.R.S. (1995). Polymerase chain reaction in food microbiology. J. Microbiol. Methods, 23(1): 89-103.
Castro-Vargas, R.E., Herrera-Sánchez M.P., Rodríguez-Hernández R. and Rondón-Barragán I.S. (2020). Antibiotic resistance in Salmonella spp. isolated from poultry: A global overview, Vet. World, 13(10): 2070-2084.
CLSI (2017). Clinical and Laboratory Standards Institute. M100-S17: Performance Standards for Antimicrobial Susceptibility Testing. 27th ed. CLSI Informational Supplement, Clinical and Laboratory Standards Institute, Wayne, PA.
Cruickshank R., Duguid J.P., Marion B.P. and Swain R.H. (1975) Medicinal microbiology, vol. II. Churchill Livingstone, London 196.
Cunha-Neto, A.D., Carvalho L.A., Carvalho R.C.T., dos Prazeres Rodrigues D., Mano S.B., de Souza Figueiredo E.E. and Conte-Junior C.A. (2018). Salmonella isolated from chicken carcasses from a slaughterhouse in the state of Mato Grosso, Brazil: antibiotic resistance profile, serotyping, and characterization by repetitive sequence-based PCR system. Poult. Sci. J., 97(4): 1373-1381.
Dagnew B., Alemayehu H., Medhin G. and Eguale T. (2020). Prevalence and antimicrobial susceptibility of Salmonella in poultry farms and in‐contact humans in Adama and Modjo towns, Ethiopia. Microbiologyopen, 9(8): e106, pp 1-9.
De Freitas Neto, O.C., PenhaFilho R.A.C., Barrow P. and Berchieri Junior A. (2010). Sources of human non-typhoid salmonellosis: a review. Braz. J. Poult. Sci., 12: 01-11.
Donado-Godoy, P., Clavijo V., León M., Arevalo A., Castellanos R., Bernal J., Tafur M. A., Ovalle M.V., Alali W.Q., Hume M. and Romero-Zuñiga J.J. (2014). Counts, serovars, and antimicrobial resistance phenotypes of Salmonella on raw chicken meat at retail in Colombia. J. Food Prot., 77(2): 227-235.
Galanis, E., Wong D.M. L. FPatrick., M. E., Binsztein N., Cieslik A., Chalermchaikit T., Aidara-Kane A., Ellis A., Angulo F. J. and Wegener H.C. (2006). Web-based surveillance and global Salmonella distribution, 2000–2002. Emerg. Infect. Dis., 12(3): 381.
Hyeon, J.Y., Chon J.W., Hwang I.G., Kwak H.S., Kim M.S., Kim S.K. and Seo K.H. (2011). Prevalence, antibiotic resistance, and molecular characterization of Salmonella serovars in retail meat products. J. Food Prot., 74(1): 161-166.
International Organization for Standardization (2002). ISO 6579:2002. Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the Detection of Salmonella spp. Geneva, Switzerland: International Standards Organisation.
Islam, M.J., Mahbub-E-Elahi A.T.M., Ahmed T. and Hasan M.K. (2016). Isolation and identification of Salmonella spp. from broiler and their antibiogram study in Sylhet, Bangladesh. J. appl. biol. biotechnol., 4(3): 46-51.
Issenhuth-Jeanjean, S., Roggentin P., Mikoleit M., Guibourdenche M., De Pinna E., Nair S. and Weill F.X. (2014). Supplement 2008–2010 (no. 48) to the white–Kauffmann–Le minor scheme. Res. Microbiol., 165(7): 526-530.
Jajere, S.M. (2019). A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multidrug resistance, Vet. World, 12(4): 504-521.
Kalambhe, D.G., Zade N.N., Chaudhari S.P., Shinde S.V., Khan W. and Patil A.R. (2016). Isolation, antibiogram and pathogenicity of Salmonella spp. recovered from slaughtered food animals in Nagpur region of Central India. Vet. world, 9(2): 176 -181.
Kaushik, P., Kumari S., Bharti S.K. and Dayal S. (2014). Isolation and prevalence of Salmonella from chicken meat and cattle milk collected from local markets of Patna, India. Vet. World, 7(2): 62.
Kumar, Y., Singh V., Kumar G., Gupta N.K. and Tahlan A.K. (2019). Serovar diversity of Salmonella among poultry. Indian J. Med. Res., 150(1): 92.
Nair, A., Balasaravanan T., Malik S.S., Mohan V., Kumar M., Vergis J. and Rawool D.B. (2015). Isolation and identification of Salmonella from diarrheagenic infants and young animals, sewage waste and fresh vegetables. Vet. world, 8(5): 669-673.
Nair, A., Balasaravanan T., Malik S.S., Mohan V., Kumar M., Vergis J. and Rawool D.B. (2015). Isolation and identification of Salmonella from diarrheagenic infants and young animals, sewage waste and fresh vegetables. Vet. world, 8(5): 669.
Pande, V.V., McWhorter A.R. and Chousalkar K.K. (2016). Salmonella enterica isolates from layer farm environments are able to form biofilm on eggshell surfaces. Biofouling, 32(7):699-710.
Pavic, A., Groves P.J. and Cox J.M. (2012). Control of Salmonella in Poultry Through Vaccination and Prophylactic Antibody Treatment. Biochemical Testing, 85-126.
Popoff, M.Y., Bockemuhl J. and Gheesling L.L. (2004). Supplement 2002 (no. 46) to the Kauffmann–White scheme. Res. Microbiol., 155(7): 568-570.
Saravanan, S., Purushothaman V., Murthy T. R. G. K., Sukumar K., Srinivasan P., Gowthaman V., Balusamy M., Atterbury R. and Kuchipudi S.V. (2015). Molecular epidemiology of Nontyphoidal Salmonella in poultry and poultry products in India: implications for human health. Indian J. Microbiol., 55(3): 319-326.
Seyfarth, A.M., Wegener H.C. and Frimodt-Moller N. (1997) Antimicrobial resistance in Salmonella enterica subsp. enterica serovar typhimurium from humans and production animals. J. Antimicrob. Chemother., 40(1): 67-75.
Shi, Q., Zhang Y., Gao G., Fang H., Miao F., Yuan Z. and He H. (2012). Phylogenetic analysis of virulence factor gene of Salmonella isolated from clinically symptomatic Chickens. Afr. J. Microbiol. Res., 6(8): 1718-1722.
Tarabees, R., Elsayed M.S., Shawish R., Basiouni S. and Shehata A.A. (2017). Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt. J. Infect. Dev. Ctries., 11(04): 314-319.
Thung, T.Y., Mahyudin N.A., Basri D.F., Radzi C.W.M., Nakaguchi Y., Nishibuchi M. and Radu S. (2016). Prevalence and antibiotic resistance of Salmonella Enteritidis and Salmonella Typhimurium in raw chicken meat at retail markets in Malaysia. Poult. Sci., 95(8): 1888-1893.
Waghamare, R.N., Paturkar A.M., Vaidya V.M., Zende R.J., Dubal Z.N., Dwivedi A. and Gaikwad R.V. (2018). Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India. Vet. World, 11(12): 1682–1688.
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