Genomic Sequence and Phylogenetic analysis of Canine Parvovirus New 2a Variant Strain in Indian Dogs
DOI:
https://doi.org/10.48165/ijvsbt.19.2.09Keywords:
Canine parvovirus (CPV), CPV-2a Strain, CPV-2b, CPV-2c and Genomic SequenceAbstract
In this study, a virulent field canine parvovirus type - 2a variant (CPV-new 2a) strain, ABT/CPV/MVC02, was identified from the faeces of a Dog with severe haemorrhagic gastroenteritis in Chennai, Tamil Nadu, India. The genomic sequence of ABT/CPV/MVC02 (MN661243) shares 99.33% nucleotide identity with those of CPV new 2a variant strains. Based on the VP2 capsid protein amino acid variation (Ser297Ala), the ABT/CPV/MVC02 strain is classified as a new CPV 2a variant strain. Two VP2 mutations Tyr324Ile and Thr440Ala were reported in most of the CPV-2a stains also present in ABT/CPV/MVC02.The phylogenetic analysis of the reported strain revealed that it was distinct from existing CPV 2a strains from India and other countries. This complete genomic analysis revealed that the CPV-2a new variant strain circulating in India is evolving rapidly with unique antigenic variations between field CPV-2a and vaccine strains which may be the major cause for vaccine failure in vaccinated dogs.
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Decaro, N., Desario, C., Addie, D.D., Martella, V., Vieira, M.J., Elia.,Davis, C., Thompson, G., Truyel, U., &Buonavoglia, C. (2007).Molecular epidemiology of canine parvovirus, Europe. Emerging Infectious Diseases, 13(8), 1222.
Horiuchi, M., Yamaguchi, Y., Gojobori, T., Mochizuki, M., Nagasawa, H., Toyoda, Y. Ishiguro, N., & Shinagawa, M. (1998).Differences in the evolutionary pattern of feline panleukopenia virus and canine parvovirus. Virology, 249(2), 440-452.
Larson, L.J., & Schultz, R.D. (2008). Do two current canine parvovirus type 2 and 2b vaccines provide protection against the new type 2c variant?.Veterinary Therapeutics, 9(2), 94.
Lee, H., Callaway, H.M., Cifuente, J.O., Bator, C.M., Parrish, C.R., &Hafenstein, S.L. (2019). Transferrin receptor binds virus capsid with dynamic motion. Proceedings of the National Academy of Sciences ofthe United States of America, 116(41), 20462–20471.
Martella, V., Decaro, N., Elia, G., &Buonavoglia, C. (2005). Surveillance activity for canine parvovirus in Italy. Journal of Veterinary Medicine, Series B, 52(7‐8), 312-315.
Mira, F., Dowgier, G., Purpari, G., Vicari, D., Di Bella, S., Macaluso, G., &Guercio, A. (2018). Molecular typing of a novel canine parvovirustype 2a mutant circulating in Italy. Infection Genetics and Evolution,61, 67–73.
Mizak, B.E.A.T.A., &Rzezutka, A.R.T.U.R. (1999). Application of nested PCR for the detection of canine parvovirus in faeces. Bulletin-Veterinary Institute in Pulawy, 43, 19-24.
Nookala, M., Mukhopadhyay, H.K., Sivaprakasam, A., Balasubramanian, B., Antony, P.X., Thanislass, J., Srinivas M.V & Pillai, R.M. (2016). Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India. ActaMicrobiologicaetImmunologicaHungarica, 63(4), 411-426.
Pereira, C.A., Leal E.S &Durigon, E.L. (2007). Selective regimen shift and demographic growth increase associated with the emergence of high-fitness variants of canine parvovirus. Infection. Genetics and Evolution.7(3), 399-409.
Phromnoi, S., Sirinarumitr, K., &Sirinarumitr, T. (2010). Sequence analysis of VP2 gene of canine parvovirus isolates in Thailand.Virus genes.41(1), 23-29.
Shackelton, L.A., Parrish, C.R., Truyen, U., & Holmes, E.C. (2005). High rate of viral evolution associated with the emergence of carnivore parvovirus. Proceedings of the National Academy of Sciences, 102(2), 379-384.
Spibey, N., Greenwood, N.M., Sutton, D., Chalmers, W.S.K., &Tarpey, I. (2008). Canine parvovirus type 2 vaccine protects against virulent challenge with type 2c virus. Veterinary microbiology, 128(1-2), 48-55.
Truyen, U. (2006). Evolution of canine parvovirus - a need for new vaccines?. Veterinary microbiology, 117(1), 9-13.
Truyen, U., Gruenberg, A., Chang, S.F., Obermaier, B., Veijalainen, P., & Parrish, C.R. (1995). Evolution of the feline-subgroup parvoviruses and the control of canine host range in vivo. Journal of Virology, 69(8), 4702–4710.
Yule, T.D., Roth, M.B., Dreier, K., Johnson, A.F., Palmer-Densmore, M., Simmons, K., &Fanton, R. (1997). Canine parvovirus vaccine elicits protection from the inflammatory and clinical consequences of the disease. Vaccine, 15(6-7), 720-729.
Zhao, H., Wang, J., Jiang, Y., Cheng, Y., Lin, P., Zhu, H., & Cheng, S. (2017). Typing of canine parvovirus strains circulating in north‐east China. Transboundary and Emerging Diseases, 64, 495–503.
Zhao, Z., Liu, H., Ding, K., Peng, C., Xue, Q., Yu, Z., &Xue, Y. (2016). Occurrence of canine parvovirus in dogs from Henan province of China in 2009‐2014. BMC Veterinary Research, 12, 138.
Zhao, Y., Lin, Y., Zeng, X., Lu, C., & Hou, J. (2013). Genotyping and pathobiologic characterization of canine parvovirus circulating in Nanjing. China Virology Journal, 10, 272.
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