Effect of Uterine Lavage, Levamisole, PGF2α and its Combinations on Haematological Indices and Bacterial Load in Estrual Mucus of Endometritic Buffaloes

Authors

  • Narendra Singh Department of Veterinary Clinical Complex, R.R. College of Veterinary and Animal Science, Deoli, Tonk, RAJUVAS, Rajasthan-304 804, India
  • Bhoopendra Singh Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and AH, Acharya Narendra Deva University of Agriculture & Technology, Ayodhya - 224 229 (UP), India
  • Rajesh Kumar Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and AH, Acharya Narendra Deva University of Agriculture & Technology, Ayodhya - 224 229 (UP), India

DOI:

https://doi.org/10.48165/ijvsbt.19.2.06

Keywords:

Buffalo, Endometritis, Levamisole, PGF2α, Uterine lavage.

Abstract

The objective of this study was to compare uterine lavage, Levamisol s/c, PGF2α i/m and its combinations on haematological indices, bacterial load in cervico-vaginal mucus (CVM) and therapeutic response in endometritic buffaloes. Thirty six repeat breeder buffaloes were randomly assigned into six equal groups. Six animals were kept as positive control (no treatment, only sexual rest, T0 ), and the rests were assigned to five treatment group, i.e., T1 (uterine lavage), T2 (Levamisol SQ ), T3 (PGF2α IM ), T4 (uterine lavage plus Levamisol s/c) and T5 (uterine lavage plus PGF2α  IM ). Significant (p<0.05) decline in bacterial load and TLC values were recorded after treatment over pretreatment ones in all five treatment groups. Furthermore, Hb, TEC and PCV increased significantly after treatment as compared to control one. Significant drop in neutrophils and rise in lymphocytes was recorded post-treatment in all treatment groups. The eosinophil, basophil and monocytes did not differ significantly within and amongst the groups. The therapeutic response was 33.33, 50.00, 66.67, 66.67, 83.33 and 83.33% in T0, T1, T2, T3, T4 and T5, respectively. The corresponding conception rates were 16.67, 33.33, 50.00, 50.00, 66.67 and 66.67 %. The results conclude that, uterine lavage along with Levamisol or PGF2α can be used effectively to manage endometrtitic buffaloes.   

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References

Ahmadi, M.R., Makki, M., Mirzaei, A., & Gheisari, H.R. (2019). Effects of hypertonic dextrose and paraffin solution as non-antibiotic treatments of clinical endometritis on reproductive performance of high producing dairy cows. Reproduction in Domestic Animals, 54(5), 762-771.

Barlund, C.S., Carruthers T.D., Waldner C.L., & Palmer C.W. (2008). A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology, 69, 714-723.

Biswal, S., Das, S., Mohanty, D.N., & Jena, D. (2014). Effect of systemic and local immunomodulation therapies on conception rate in endometritic cows. Indian Journal of Field Veterinarians, 10(2), 1-4.

Brinsko, S.P., Blanchard, T.L., Varner, D.D., Schumacher, J., Love, C.C., Hinrichs, K., & Hartman, D.L. (2011). Endometritis. Manual of Equine Reproduction, 73-84.

Butani, M.G., Dhami, A.J., Kumar, R., Hirani, N.D., Ramani, V.P., & Patel, K.P. (2009). Influence of hormonal and antibiotics therapy on fertility and trace minerals profile in repeat breeding buffaloes. Indian Journal of Field Veterinarians, 4(3), 12-16.

Dash, S., Basu, S., Mishra, P.C., & Ray, K. (2019). Effect of immunomodulators on certain haematological parameters in ameliorating bovine Endometritis. International Journal of Chemical Studies, 7(2), 1736-1739

Dutt Ravi, Singh, G., Singh, M., Sharma, M., Dalal, J., & Chandolia, R.K. (2017). Diagnosis of subclinical endometritis in Murrah buffaloes through Cytobrush technique. International Journal of Current Microbiology and Applied Sciences, 6(11), 494-499.

Feldman, B.F, Zinkl, J.G., & Jain, N.C. (2000). Schalm’s Veterinary Hematology. 5th ed., Lippincott Williams and Wilkins, Philadelphia.

Heidarpour, M., Mohri, M., Fallah-Rad, A.H., Shahreza, D.F., & Mohammadi, M. (2014). Hematological changes before and after treatment in dairy cows with clinical and subclinical endometritis. Comparative Clinical Pathology, 23(1), 97-101.

Kasimanickam, R., Duffield, T.F., Foster, R.A., Gartley, C.J., Leslie, K.E., Walton, J.S., & Johnson, W.H. (2004). Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology, 62, 9-23.

Kekan, P.M., Shirbhate, R.N., & Nimbulkar, M.V. (2005). Haematological studies during estrous cycle in regular and repeat breeding cows. Indian Veterinary Journal, 82(7), 805-806.

Kumar, R., Butani, M.G., Dhami, A.J., Kavani, F.S., Bhong, C.D., & Brahmbhatt, M.N. (2008). Isolation and antibiogram of genital microflora from repeat breeding cows and buffaloes. Indian Journal of Field Veterinarians, 4(2), 5-9.

Kumar, R., Dhami, A.J., Butani, M.G., Kavani, F.S., Sarvaiya, N.P., & Killledar, A. (2010). Management of repeat breeding under field condition using hormonal and non hormonal drugs in cows. Indian Journal of Animal Reproduction, 31 (1), 30-32.

LeBlanc, S.J., Duffield, T.F., Leslie, K.E., Bateman, K.G., Keefe, G.P., Walton, J.S. & Johnson W.H. (2002). Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. Journal of Dairy Science, 85, 2223-2236.

Lee, S.C., Jeong, K.J., Choi, I.S., Kang, H.G., Jung, Y.H., Park, S.B., & Kim, I.H. (2018). Cytological endometritis in dairy cows: diagnostic threshold, risk factors, and impact on reproductive performance. Journal of Veterinary Science, 19(2), 301-308.

Liu, I., & Troedsson, M. (2008). The diagnosis and treatment of endometritis in the mare: yesterday and today. Theriogenology, 70, 415-420.

Makki, M., Ahmadi, M.R., Gheisari, H.R., & Nazifi, S. (2017). Cure rate of postpartum endometritis after different treatments in high produce dairy cows. Comparative Clinical Pathology, 26, 921-928.

Palanisamy, M., Ezakial Napolean, R., Selvaraju, M., Balasubramanian, G.A. Krishnakumar, K. and Manokaran, S. (2015) Efficacy of immunomodulators, Lugol’s iodine and PGF2α on bacterial load in the endometritis affected cows. Indian Veterinary Journal, 92(7), 56-58.

Patil, R.D. (2010). Studies on haemato-biochemical, hormonal and therapeutic aspects of postpartum metritis in Surti buffaloes. MVSc thesis. Anand Agricultural University, Gujarat (India).

Patil, R.D., Patel, J.A., Hadiya, K.K., & Dhami, A.J. (2015). Comprehensive haematological alterations following various therapies in Surti buffaloes with postpartum metritis. Indian Journal of Animal Reproduction, 36(1), 1-4.

Perumal, P., Das, S., Mohanty, D.N., Barik, A.K., & Mishra, P.C. (2013). Study of certain haematological parameters in repeat breeding cows. International Journal of Bio-resource and Stress Management, 4(2), 242-245.

Reddy, N.C.S., Bramhaiah, K.V., Naidu, K.S., Babu, A.J., & Kumar, R.V.S. (2012). Effect of uterine lavage on bacterial count and conception rate in repeat breeder crossbred cows. Indian Journal of Animal Reproduction, 33(1), 59-62.

Sabasthin, A., Kumar, V.G., Nandi, S., & Murthy, V.C. (2012). Blood hematological and biochemical parameters in normal cycling, pregnant and repeat breeding buffaloes (Bubalus bubalis) maintained in isothermic and isonutritional conditions. Asian Pacific Journal of Reproduction, 1(2), 117-119.

Samatha, V., & Ramesh, B.K. (2013). Bacteriological and Cytological studies of endometritis in buffaloes. Internationa journal of food, Agriculture and Veterinary Sciences, 3(2), 6-9.

Sheldon, I.M., Noakes, D.E., Rycroft, A.N., & Dobson, H. (2004). Effect of intrauterine administration of oestradiol on postpartum uterine bacterial infection in cattle. Animal Reproduction Science, 81, 13-23.

Singh, B., Gupta, H.P., Prasad, S., & Rajora, V.S. (2018). Effect of Immunomodulators on serum nitric oxide concentration of endometritic repeat breeding crossbred cows. Indian Journal of Veterinary Research, 27(1), 32-35.

Singh, P.P., Pande, N., Bhavna, & Agrawal, R. (2017). Clinical and biochemical studies on endometrtitic repeat breeding cows following treatment with levamisol. The Haryana Veterinarian, 56(1), 55-57.

Snedecor, G.W., & Cochran, W.G. (1994). Statistical methods. 8th edition, Iowa State University Press, Ames, Iowa, USA,

Sultana, J., Khan, S.A., Pasha, M.R., Imtiaz, M.A., Saif, A., Nova, F., & Shaikat, A.H. (2022). Alteration of physiological parameters in repeat breeder cows. Asian Journal of Advances in Research, 15(2), 1-5.

Swain, P.K., Mohanty, D.N., Das, S., Barik, A.K., Mishra, P.C., Tripathi, A.K., & Palai, T.K. (2011). Immunomodulation effect of levamisole and immulite on uterine microbial picture in repeat breeding cows. Indian Journal of Animal Reproduction, 32(3), 68-70.

Thrall, M.A. (2004). Veterinary Haematology and Clinical Chemistry. Published by Lippincott Williams and Wilkins, Philadelphia, p. 71, 84-85.

Wang, H., Yan, Z., Wu, X., Zhang, Y., Wei, Y., & Zhao, X. (2021). Using vaginal discharge score (VDS) grading system to evaluate the effect of clinical endometritis on reproductive performance of dairy cows in China. Animal Reproduction, 18(1), 1-12.

Westermann, S., Drillich, M., Kaufmann, T.B., Madoz, L.V., Heuwieser, W. (2010). A clinical approach to determine false positive findings of clinical endometritis by vaginoscopy by the use of uterine bacteriology and cytology in dairy cows. Theriogenology, 74, 1248-1255.

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Published

2023-03-02

How to Cite

Singh, N., Singh, B., & Kumar, R. (2023). Effect of Uterine Lavage, Levamisole, PGF2α and its Combinations on Haematological Indices and Bacterial Load in Estrual Mucus of Endometritic Buffaloes. Indian Journal of Veterinary Sciences and Biotechnology, 19(2), 28–33. https://doi.org/10.48165/ijvsbt.19.2.06