Isolation, Identification and Antibiogram of Cloacal Flora from Apparently Healthy Caged Zoo Birds

Authors

  • Akash V. Golaviya Sri Sayajibaugh Zoo, Vadodara Municipal Corporation, Vadodara-390018, Gujarat, India
  • Suchit S. Pandya Sri Sayajibaugh Zoo, Vadodara Municipal Corporation, Vadodara-390018, Gujarat, India
  • Pratyush G. Patankar Sri Sayajibaugh Zoo, Vadodara Municipal Corporation, Vadodara-390018, Gujarat, India
  • Harshrajsinh B. Joddha Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Chirag M. Chavda Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Bharat B. Bhanderi Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India
  • Mayurdhvaj K. Jhala Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.19.1.09

Keywords:

Antibiotic sensitivity, Cloacal flora, Escherichia coli, Zoo birds

Abstract

Antibiotics are routinely used in animals and birds to prevent and cure bacterial infections. Various multidrug resistance bacteria have been detected in caged zoo birds, however there is a little known about their resistance pattern. In current study we isolated and identified cloacal bacteria from 50 zoo birds and compared their antimicrobial susceptibility. A total of 28 cloacal samples (56%) were found positive for different bacteria by selective culture. The bacteria isolated from different types of caged birds were Escherichia coli (26%), Staphylococcus spp. (8%), Streptococcus spp. (4%) and some unidentified Gram-positive and Gram-negative bacteria (14%). Of these isolates, E. coli was the most frequent isolate. The results of antibiotic sensitivity tests revealed that cefotaxime and sulphadiazine were highly (>60%) resistant to isolated cloacal microflora of zoo birds. However, the antibiotics such as ceftriaxone and amikacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, co-trimoxazole was found to be consistently highly effective (100%) on all the E. coli isolates. Overall, the results showed that zoo birds can be carrier of multidrug resistant organisms including Escherichia coli.

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References

Abdallah, S.M., & Khalil, S.A. (2016). Molecular studies on Escherichia coli serotypes in Zoo birds. Alexandria Journal for Veterinary Sciences, 51(2), 7-14.

Akhter, J., Hossain, M.T., Islam, M.T., Siddique, M.P., & Islam, M.A. (2010). Isolation and identification of microflora from apparently healthy caged parrots of Dhaka Zoo of Bangladesh. Bangladesh Journal of Veterinary Medicine, 8(1), 05-10.

Beleza, A.J.F., Maciel, W.C., Carreira, A.S., Marques, A.R., Nogueira, C.H.G., Pascoal Filho, N.M., ... & de Castro Teixeira, R.S. (2021). Wild birds as reservoirs of multidrug-resistant enterobacteria in Mulungu, Brazil. Bio Rxiv., 1-29.

Borges, C.A., Beraldo, L.G., Maluta, R.P., Cardozo, M.V., Barboza, K.B., Guastalli, E.A.L., ... & Ávila, F.A. (2017). Multidrug-resistant pathogenic Escherichia coli isolated from wild birds in a veterinary hospital. Avian Pathology, 46(1), 76-83.

CLSI - Clinical Laboratory Standards Institute (2022). M100-S22: performance standards for antimicrobial susceptibility testing; thirty second informational supplement -M100S22E.pdf. M100Ed32 | Performance Standards for Antimicrobial Susceptibility Testing, 32nd Edition (clsi.org).

Cruickshank, R., Duguid, J., Marmion, B.P., & Swain, (1975). Medical Microbiology. The practice of medical microbiology, 12th Edn, Vol. II. Churchill. Edinburgh.

Doneley R.T. (2009). Bacterial and parasitic diseases of parrots. Veterinary Clinics of North America: Exotic Animal Practice, 12(3), 417-432.

Edwards, R. & Ewing, W.N. (1972). Identification of Enterobacteriaceae. 3rd edn., Burgess Publishing Co., Minnesota

Hedawy, K., & El-Shorbagy, M. (2007). Role of some wild birds in transmitting some Bacterial agents among poultry farms in Sohag Governorate. Assuit Veterinary Medicine Journal, 53, 251-257.

Legadevi, R., Dillibabu, V., Karunakaran, N., Nagarajan, K., & Soundararajan, C. (2019). Isolation and identification of bacteria of normal flora in handfed exotic psittacine birds at Tamil Nadu, India. Indian Journal of Veterinary & Animal Science Research, 48(2), 18-25.

Literak, I., Dolejska, M., Janoszowska, D., Hrusakova, J., Meissner, W., Rzyska, H., ... & Cizek, A. (2010). Antibiotic-resistant Escherichia coli bacteria, including strains with genes encoding the extended-spectrum beta-lactamase and QnrS, in waterbirds on the Baltic Sea Coast of Poland. Applied and Environmental Microbiology, 76(24), 8126-8134.

Markey, B., Leonard, F., Archambault, M., Cullinane, A., & Maguire, D. (2013). Clinical Veterinary Microbiology. 2nd Ed. UK, Elsevier Health. ISBN – 9780702055881.

Nolan, L. K., Barnes, H. J., Vaillancourt, J. P., Abdul-Aziz, T., & Logue, C. M. (2020). Colibacillosis. In: Diseases of Poultry, p. 770-830. Blackwell publishing, United States.

Nowaczek, A., Dec, M., Stępień-Pyśniak, D., Urban-Chmiel, R., Marek, A., & Różański, P. (2021). Antibiotic resistance and virulence profiles of Escherichia coli strains isolated from wild birds in Poland. Pathogens, 10(8), 1059.

Pinto, A., Simões, R., Oliveira, M., Vaz-Pires, P., Brandão, R., & da Costa, P. M. (2015). Multidrug resistance in wild bird populations: importance of the food chain. Journal of Zoo and Wildlife Medicine, 46(4), 723-731.

Radhouani, H., Poeta, P., Goncalves, A., Pacheco, R., Sargo, R., & Igrejas, G. (2012). Wild birds as biological indicators of environmental pollution: antimicrobial resistance patterns of Escherichia coli and Enterococci isolated from common buzzards (Buteo buteo). Journal of Medical Microbiology, 61(6), 837-843.

Rogers, K.H. (2006). Prevalence of pathogenic enteric bacteria in wild birds associated with agriculture in Humboldt County, California (Master's thesis, Humboldt State University). http://hdl.handle.net/2148/100

Sigirci, B.D., Celik, B., Halac, B., Adiguzel, M.C., Kekec, I., Metiner, K., ... & Kahraman, B. B. (2020). Antimicrobial resistance profiles of Escherichia coli isolated from companion birds. Journal of King Saud University-Science, 32(1), 1069-1073.

Suárez-Pérez, A., Corbera, J.A., González-Martín, M., Donázar, J.A., Rosales, R.S., Morales, M., & Tejedor-Junco, M.T. (2020). Microorganisms resistant to antimicrobials in wild canarianegyptian vultures (Neophronpercnopterus majorensis). Animals, 10(6), 970.

Suphoronski, S.A., de Freitas Raso, T., Weinert, N.C., Seki, M.C., & Carrasco, A.D.O.T. (2015). Occurrence of Salmonella sp. and Escherichia coli in free-living and captive wild birds from 2010-2013 in Guarapuava, Paran, Brazil. African Journal of Microbiology Research, 9(29), 1778-1782.

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Published

2023-01-10

Issue

Vol. 19 No. 1 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan- Feb) 2023

Section

Articles

License

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Golaviya, A.V., Pandya, .S.S., Patankar, P.G., Joddha, H.B., Chavda, C.M., Bhanderi, B.B., & Jhala, M.K. (2023). Isolation, Identification and Antibiogram of Cloacal Flora from Apparently Healthy Caged Zoo Birds . Indian Journal of Veterinary Sciences and Biotechnology, 19(1), 38–42. https://doi.org/10.48165/ijvsbt.19.1.09
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