Molecular Detection and Genetic Analysis of Lumpy Skin Disease Virus (LSDV) in India

Authors

  • Mouttou Vivek Srinivas Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India.
  • Uma Sambath Department of Veterinary Pathology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry -605 009, India.
  • Selvi Darmalingam Department of Veterinary Medicine, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry -605 009, India.
  • Jayalakshmi Vasu Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India.
  • Hirak Kumar Mukhopadhyay Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry-605 009, India.

DOI:

https://doi.org/10.48165/ijvsbt.19.1.05

Keywords:

Capripoxvirus, Lumpy skin disease virus (LSDV), Phylogenetic analysis, P32 gene, Polymerase chain reaction, Sequence analysis

Abstract

Lumpy skin disease (LSD) is a contagious trans-boundary viral disease of cattle, caused by lumpy skin disease virus (LSDV) under the genus Capripoxvirus of Poxviridae family. The genus Capripoxvirus also comprises goatpox virus (GTPV) and sheeppox virus (SPPV), which affect sheep and goat respectively and are antigenically similar to LSDV. LSD has recently been spread in Asia following outbreaks in the Middle East and Europe. The present study is aimed with the objective of molecular characterization and genetic analysis of LSD virus obtained from the infected cattle in Puducherry, India. PCR confirms the presence of the LSDV genome in the clinical sample. Phylogenetic analysis and complete assessment of multiple sequence alignments between LSDV, GTPV and SPPV P32 gene sequences revealed least variation between sheeppox virus and LSD virus (1.5% variation) and highest variation between sheeppox and goatpox virus (2.8% variation). Based on the present study, it is also confirmed and concluded that P32 gene can be used for differentiating LSD, sheeppox and goatpox viruses. The phylogenetic analysis revealed that the LSDV under this study was clustered in a separate clade with the other LSDV sequences of Turkey, Kazakhstan, Kenya, Russia, Serbia, Nigeria and other Indian LSDV sequences available in the GenBank. The query LSDV sequence was closely related with other LSD viruses circulating in India suggesting a single LSDV strain is circulating in the country. This result underlines the importance of continuous monitoring and characterization of circulating strains.

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References

Abutarbush, S. M., & Tuppurainen, E. S. M. (2018). Serological and clinical evaluation of the Yugoslavian RM65 sheep pox strain vaccine use in cattle against lumpy skin disease. Transboundary and Emerging Diseases, 65, 1657–1663.

Alemayehu, G., Zewde, G., & Admassu, B. (2013). Risk assessments of lumpy skin diseases in Borena bull market chain and its implication for livelihoods and international trade. Tropical Animal Health and Production, 45, 1153–1159.

Badhy, S. C., Chowdhury, M. G. A., Settypalli, T. B. K., Cattoli, G., Lamien, C. E., Fakir, M. A. U., & Sadekuzzaman, M. (2021). Molecular characterization of lumpy skin disease virus (LSDV) emerged in Bangladesh reveals unique genetic features compared to contemporary field strains. BMC Veterinary Research, 17, 61.

Bhaswanth, K., Vivek Srinivas, V. M., Selvi, D., Jayalakshmi, V., Antony, P. X., & Mukhopadhyay, H. K. (2020). P32 Gene based molecular characterization of Goatpox virus. Veterinary Research International, 8 (1), 28-32.

Bowden, T. R., Babiuk, S. L., Parkyn, G. R., Copps, J. S., & Boyle, D. B. (2008). Capripoxvirus tissue tropism and shedding: a quantitative study in experimentally infected sheep and goats. Virology, 371, 380–93.

Casal, J., Allepuz, A., Miteva, A., Pite, L., & Tabakovsky, B. (2018). Economic cost of lumpy skin disease outbreaks in three Balkan countries: Albania, Bulgaria and the Former Yugoslav Republic of Macedonia (2016–2017). Transboundary and Emerging Diseases, 65, 1680–1688.

CABI. (2019). Lumpy skin Disease. https://wwwcabiorg/isc/datasheet/76780.

Chand, P., Kitching, R. P., & Black, D. N. (1994). Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep. Epidemiology and Infection, 113, 377-385

Davies, F. G. (1991). Lumpy skin disease, an African capripox virus disease of cattle. British Veterinary Journal, 147, 489–503.

Edelsten, M. (2014). Threat to European cattle from lumpy skin disease. Veterinary Record, 175, 330.

Gubser, C., Hué, S., Kellam, P., & Smith, G. L. (2003). Poxvirus genomes: a phylogenetic analysis. Journal of General Virology, 85(1), 105-117.

Hosamani, M., Mondal, B., Tembhurne, P. A., Bandyopadhyay, S. K., Singh, R. K., & Rasool, T. J. (2004). Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene. Virus genes, 29(1), 73-80.

Lojkic, I., Simic, I., Kresic, N., & Bedekovic, T. (2018). Complete Genome Sequence of a Lumpy Skin Disease Virus Strain Isolated from the Skin of a Vaccinated Animal. Genome Announcement, 6(22), e00482-18.

MacDonald, R. A. S. (1931). Pseudo-urticaria of cattle. Annual Report for 1930–1931. Department of Animal Health, Government of Northern Rhodesia; Lusaka, Zambia, 2–21.

OIE (2021). Lumpy skin disease in Hong Kong. https://rr-asia.oie.int/wp-content/uploads/2021/01/3-lumpy-skin-disease-in-hong-kong-18-12-2020-afcd.pdf.

Shen, Y. J., Shephard, E., Douglass, N., Johnston, N., Adams, C., & Williamson, C. (2011). A novel candidate HIV vaccine vector based on the replication deficient Capripoxvirus, lumpy skin disease virus (LSDV). Virology Journal, 8, 1–2.

Sprygin, A., Pestova, Y., Wallace, D. B., Tuppurainen, E., & Kononov, A. V. (2019). Transmission of lumpy skin disease virus: A short review. Virus Research, 269, 197637.

Sudhakar, S. B., Mishra, N., Kalaiyarasu, S., Jhade, S. K., & Hemadri, D. (2020). Lumpy skin disease (LSD) outbreaks in cattle in Odisha state, India in August 2019: Epidemiological features and molecular studies. Transboundary and Emerging Diseases, 67(6), 2408-2422

Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, 38(7): 3022–3027

Tasioudi, K. E., Antoniou, S. E., Iliadou, P., Sachpatzidis, A., & Plevraki, E. (2016). Emergence of Lumpy Skin Disease in Greece, 2015. Transboundary and Emerging Diseases, 63, 260–265.

Tuppurainen, E. S. M., & Oura, C. A. (2012). Review: lumpy skin disease: an emerging threat to Europe, the Middle East and Asia. Transboundary and Emerging Diseases, 59, 40–48.

Yeruham, I., Nir, O., Braverman, Y., Davidson, M., & Grinstein, H. (1995). Spread of lumpy skin disease in Israeli dairy herds. Veterinary Record, 137, 91–93.

Yilmaz, H. (2017). Lumpy Skin Disease: Global and Turkish Perspectives. Approaches in Poultry, Dairy & Veterinary Sciences, 1,11-15.

Zhou, T., Jia, H., Chen, G., He, X., Fang, Y., Wang, X., Guan, Q., Zeng, S., Cui, Q., & Jing, Z. (2012). Phylogenetic analysis of Chinese sheeppox and goatpox virus isolates. Virology Journal, 9, 25.

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Published

2023-01-10

Issue

Vol. 19 No. 1 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan- Feb) 2023

Section

Articles

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Srinivas, M.V., Sambath, U., Darmalingam, S., Vasu, J., & Mukhopadhyay, H.K. (2023). Molecular Detection and Genetic Analysis of Lumpy Skin Disease Virus (LSDV) in India. Indian Journal of Veterinary Sciences and Biotechnology, 19(1), 18–22. https://doi.org/10.48165/ijvsbt.19.1.05
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