Expression Pattern of SLIT 2 Genes Discriminates Malignant from Benign Canine Mammary Tumours

Authors

  • Sandeep Saharan Department of Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary & Animal Sciences (LUVAS), Hisar-125004, India
  • Prem Singh Department of Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary & Animal Sciences (LUVAS), Hisar-125004, India
  • Ajit Singh Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary & Animal Sciences (LUVAS), Hisar-125004, India
  • Rajendra Prasad Gupta Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary & Animal Sciences (LUVAS), Hisar-125004, India
  • Ravi Dutt Department of Veterinary Gynaecology & Obstetrics, Lala Lajpat Rai University of Veterinary & Animal Sciences (LUVAS), Hisar-125004, India

DOI:

https://doi.org/10.48165/ijvsbt.19.1.01

Keywords:

Histopathology, Mammary tumours, Quantitative real-time PCR, SLIT2 genes

Abstract

The study was conducted to investigate the expression of SLIT2 gene in representative tissue samples of mammary tumours of female dogs (n=10) with the age between 5.0 and 16.5 years brought to Veterinary Clinical Complex, LUVAS, Hisar. Excisional biopsy was taken after surgery of these cases. Histopathological studies confirmed four cases of adenocarcinoma, three cases of benign mixed tumours and two of myoepithelioma and one case of adenoma. Amplification plots and dissociation curves of RT-qPCR were indicative of specific amplification of the canine SLIT 2 gene in all the samples. Further, amplification product of a 160 bp SLIT 2 gene and 120 bp β-actin gene in all the RT-qPCR tubes were visible by 2% agarose gel electrophoresis. Out of 10 tissue samples examined, transcript levels of SLIT 2 genes in all different samples ranged from 1.90 to 84.98. Conclusively, the expression of SLIT 2 genes increased in all canine malignant mammary gland tumours, thereby showing its association with tumour malignancy.

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References

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Published

2023-01-10

Issue

Vol. 19 No. 1 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan- Feb) 2023

Section

Articles

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Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Saharan, S., Singh, P., Singh, A., Gupta, R.P., & Dutt, .R. (2023). Expression Pattern of SLIT 2 Genes Discriminates Malignant from Benign Canine Mammary Tumours . Indian Journal of Veterinary Sciences and Biotechnology, 19(1), 1–4. https://doi.org/10.48165/ijvsbt.19.1.01
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