Comparative Blood Profile Of Progesterone, Metabolites And Minerals In Anoestrus, Suboestrus, Repeat Breeding And Normal Cyclic Buffaloes

Abstract

A comparative study on serum profile of progesterone (P4) and metabolites of 37 anoestrus, 10 suboestrus, 53 repeat breeding and 12 normal cyclic buffaloes revealed that the serum P4 was significantly (P<0.01) higher in suboestrus (3.36±0.50 ng/ml) as compared to anoestrus, repeat breeders and normal cyclic buffaloes (1.24±0.21, 0.45±0.05 and 0.36±0.08 ng/ml). The serum total protein in anoestrus, suboestrus and repeat breeding buffaloes was significantly (P<0.05) lower (8.69±0.25, 9.62±0.59 and 9.06±0.21 g/dl) than that in the normal cyclic buffaloes (11.05±0.30 g/ dl). Cholesterol was lower (P<0.01) in anoestrus and repeat breeding buffaloes (168.82±8.14 and 143.97±7.49 mg/dl) as compared to suboestrus and normal cyclic ones (184.84±14.83 and 183.09±11.70 mg/dl). The calcium levels though low in infertile buffaloes did not differ significantly from normal cyclic buffaloes. The serum magnesium content of normal cyclic buffaloes was significantly higher (4.18±0.11 mEq/L) than that of all three categories of infertile animals. The serum trace minerals, zinc, iron and manganese levels did not differ significantly among different groups of infertile and normal cyclic buffaloes. However, the serum copper level in repeat breeders was significantly (P<0.05) higher (1.10±0.06 ppm) and cobalt was lower (0.08±0.01 ppm) as compared to anoestrus, suboestrus and even normal cyclic buffaloes. The findings suggested the importance of the above elements in maintaining normal reproductive rhythm of buffaloes.