Seasonal Influence on Efficacy of Ovsynch, Heatsynch and Triu-B Protocols in Terms of Estrus Response, Conception Rate and Biochemical Profile in Postpartum Anoestrus Buffaloes

Abstract

This study was undertaken on 48 postpartum (>90 days) anoestrus buffaloes each in winter and  summer seasons with the object to know the seasonal influence on efficacy of three estrus induction/  synchronization protocols towards fertility response and blood biochemical profiles. Twelve buffaloes  each in treatment group I, II and Ill were subjected to Ovsynch, Heatsynch and Triu-B hormone  protocols, respectively, while group IV served as untreated control. The estrus induction response  varied from 75.00 to 83.33% during summer and 83.33 to 91 .66% during winter season with these  protocols. The conception rates obtained at induced estrus and overall of 3 cycles in anoestrus  buffaloes were comparatively lower under all three protocols during summer than winter season  (16.27 vs 41.67 & 25.00 vs 58.33% for Ovsynch; 16.27 vs 25.00 &25.00 vs 50.00% for Heatsynch,  and 16.27 vs 41 .67 & 33.33 vs 58.33%, respectively), and obviously higher than control group  (overall CR 8.33 & 25.00%} in both the seasons. The overall mean plasma protein values were  significantly higher (p<0.01) in winter season than summer season in all three groups, while inverse  results were found with respect to seasons for total cholesterol under Ovsynch and Triu-B protocols.  The effect of period and pregnancy status was non-significant within the protocol for both the traits  in both the seasons, except the total protein under Ovsynch protocol in winter season was  significantly higher in conceived than non-conceived group (7.96 ± 0.09 vs. 7.54 ± 0.11 g/dl; p<0.05).  There was no influence of days or conceived and non-conceived status on the calcium, phosphorus  and magnesium profile in any of the groups studied during both the seasons. However, significantly  higher (p<0.05) overall mean plasma values for calcium and magnesium were observed in winter  as compared to summer season in buffaloes of all three protocols. It was concluded that all three  protocols can be used successfully during winter and Triu-B only during summer season to induce  estrus and improve fertility in postpartum anoestrus buffaloes, and that the plasma metabolite profile  was significantly altered by season, but not by the hormonal treatments or pregnancy status.