Estrus Induction with Exogenous Melatonin in Anestrus Pubertal Jaffarabadi Buffalo Heifers during Summer Season
DOI:
https://doi.org/10.48165/ijvsbt.22.1.06Keywords:
Buffalo heifers, Estrus induction, Follicular dynamics, Melatonin, Oxidative stress, ProgesteroneAbstract
The present study evaluated the effect of exogenous melatonin on ovarian follicular dynamics, hormonal profile, oxidative stress, and conception rate in pubertal Jaffarabadi buffalo heifers during the summer season. Twelve buffalo heifers (32-36 months; 300-350 kg) were randomly divided into two equal groups: Control (Group I; corn oil 1 mL/50 kg s/c) and Melatonin-treated (Group II; melatonin 18 mg/50 kg b.wt. in corn oil s/c). Ovarian activity was monitored using ultrasonography, and blood samples were analyzed for melatonin, progesterone, lipid peroxidation (LPO), and total antioxidant capacity (TAC). Melatonin significantly (p<0.05) enhanced follicular recruitment and follicle diameter, reduced oxidative stress, increased TAC, and improved estrus induction and conception rate. The study demonstrates melatonin’s beneficial role in alleviating heat stress-induced reproductive suppression and improving fertility in buffalo heifers.
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Abulaiti, A., Nawaz, M., Naseer, Z., Ahmed, Z., Liu, W., Abdelrahman, M., & Wang, S. (2023). Administration of melatonin prior to modified synchronization protocol improves the productive and reproductive efficiency of Chinese crossbred buffaloes in low breeding season. Frontiers in Veterinary Science, 10, 1118604.
Bashandy, S.A., Ebaid, H., Al-Tamimi, J., Ahmed-Farid, O.A.H., Omara, E.A., & Alhazza, I.M. (2021). Melatonin alleviated potassium dichromate-induced oxidative stress and reprotoxicity in male rats. BioMed Research International, 1, 3565360.
Benot, S., Molinero, P., Soutto, M., Goberna, R., & Guerrero, J.M. (1998). Circadian variations in the rat serum total antioxidant status: Correlation with melatonin levels. Journal of Pineal Research, 25(1), 1–4.
Das, G.K., & Khan, F.A. (2010). Summer anoestrus in buffalo – A review. Reproduction in Domestic Animals, 45(6), 483–494.
Dash, S., Chakravarty, A.K., Sah, V., Jamuna, V., Behera, R., Kashyap, N., & Deshmukh, B. (2015). Influence of temperature and humidity on pregnancy rate of Murrah buffaloes under subtropical climate. Asian-Australasian Journal of Animal Sciences, 28(7), 943.
Deb, G.K., Nahar, T.N., Duran, P.G., & Presicce, G.A. (2016). Safe and sustainable traditional production: The water buffalo in Asia. Frontiers in Environmental Science, 4, 38.
El-Raey, M., Geshi, M., Somfai, T., Kaneda, M., Hirako, M., Abdel-Ghaffar, A.E., & Nagai, T. (2011). Evidence of melatonin synthesis in the cumulus–oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle. Molecular Reproduction and Development, 78(4), 250–262.
Ghuman, S.P.S., Singh, J., Honparkhe, M., Dadarwal, D., Dhaliwal, G.S., & Jain, A.K. (2010). Induction of ovulation of ovulatory size non-ovulatory follicles and initiation of ovarian cyclicity in summer anoestrous buffalo heifers (Bubalus bubalis) using melatonin implants. Reproduction in Domestic Animals, 45(4), 600–607.
Kumar, A., Mehrotra, S., Singh, G., Maurya, V.P., Narayanan, K., Mahla, A.S., Chaudhary, R.K., Singh, M., Soni, Y.K., Kumawat, B.L., Dabas, S.K., & Srivastava, N. (2016). Supplementation of slow-release melatonin improves recovery of ovarian cyclicity and conception in summer anoestrous buffaloes (Bubalus bubalis). Reproduction in Domestic Animals, 51(1), 10–17.
Kumar, A., Mehrotra, S., Singh, G., Narayanan, K., Das, G.K., Soni, Y.K., & Verma, M.R. (2015). Sustained delivery of exogenous melatonin influences biomarkers of oxidative stress and total antioxidant capacity in summer-stressed anestrous water buffalo (Bubalus bubalis). Theriogenology, 83(9), 1402–1407.
Malpaux, B., Migaud, M., Tricoire, H., & Chemineau, P. (2001). Biology of mammalian photoperiodism and the critical role of the pineal gland and melatonin. Journal of Biological Rhythms, 16(4), 336–347.
Mota-Rojas, D., Braghieri, A., Alvarez-Macías, A., Serrapica, F., Ramirez-Bribiesca, E., Cruz-Monterrosa, R., & Napolitano, F. (2021). The use of draught animals in rural labour. Animals, 11(9), 2683.
Phogat, J.B., Pandey, A.K., & Singh, I. (2016). Seasonality in buffaloes reproduction. International Journal of Plant, Animal and Environmental Sciences, 6(2), 46–54.
Phogat, J.B., Pandey, A.K., Balhara, A.K., Ghuman, S.S., & Gunwant, P. (2018). Effects of melatonin supplementation prior to Ovsynch protocol on ovarian activity and conception rates in anestrous Murrah buffalo heifers during out-of-breeding season. Reproductive Biology, 18(2), 161–168.
Ramadan, T.A., Sharma, R.K., Phulia, S.K., Balhara, A.K., Ghuman, S.S., & Singh, I. (2014). Effectiveness of melatonin and controlled internal drug release device treatment on reproductive performance of buffalo heifers during out-of-breeding season under tropical conditions. Theriogenology, 82(9), 1296–1302.
Ratchamak, R., Thananurak, P., Boonkum, W., Semaming, Y., & Chankitisakul, V. (2022). Melatonin treatment improves ovarian responses after superstimulation in Thai-Holstein crossbreds under heat stress. Frontiers in Veterinary Science, 9, 888039.
Reiter, R.J., Tan, D.X., Manchester, L.C., Paredes, S.D., Mayo, J.C., & Sainz, R.M. (2009). Melatonin and reproduction revisited. Biology of Reproduction, 81(3), 445–456.
Snedecor, G.W., & Cochran, W.G. (1994). Statistical Methods (6th ed.). Oxford & IBH Publishing Co., Calcutta, India.
Tamura, H., Takasaki, A., Taketani, T., Tanabe, M., Kizuka, F., Lee, L., & Sugino, N. (2012). The role of melatonin as an antioxidant in the follicle. Journal of Ovarian Research, 5(1), 5.
Venegas, C., Garcia, J.A., Escames, G., Ortiz, F., Lopez, A., Doerrier, C., & Acuña-Castroviejo, D. (2012). Extrapineal melatonin: Analysis of its subcellular distribution and daily fluctuations. Journal of Pineal Research, 52(2), 217–227.
Warriach, H.M., McGill, D.M., Bush, R.D., Wynn, P.C., & Chohan, K.R. (2015). A review of recent developments in buffalo reproduction. Asian-Australasian Journal of Animal Sciences, 28(3), 451–455.
Yang, M., Shi, J., Tao, J., Wu, H.C.M., & Wang, J. (2017). Effects of different melatonin injection ways on the enrichment and metabolism of blood melatonin in Holstein cows. China Animal Husbandry & Veterinary Medicine, 9, 56489.
Zarezadeh, M., Barzegari, M., Aghapour, B., Adeli, S., Khademi, F., Musazadeh, V., & Ostadrahimi, A. (2022). Melatonin effectiveness in amelioration of oxidative stress and strengthening of antioxidant defense system: A systematic review and dose–response meta-analysis. Clinical Nutrition, 48, 109–120.
Zicarelli, L. (1997). Reproductive seasonality in buffalo. Bubalus Bubalis, 4(1), 29–52.
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