Glutathione as a Semen Additive to Improve Surti Buffalo (Bubalus bubalis) Bull Semen Cryopreservation

Authors

  • Kailash Kumar Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001, India
  • Mitesh Gaur Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001, India

Keywords:

Cryopreservation, Glutathione, Post-thaw quality, Surti buffalo, Semen additive

Abstract

The present study evaluated the effects of glutathione as an additive in Tris fructose egg yolk citrate extender on Surti buffalo bull semen.  Extended semen @ 80 million sperm/mL was divided into 4 equal aliquots and glutathione was added at 0.5 mM (T1), 2.0 mM (T2), and  5.0 mM (T3) with one control (T0) group. The percentages of post-thaw sperm progressive motility, live sperm, and HOS responsive  sperm were significantly higher (p <0.05), whereas sperm abnormalities were significantly lower (p <0.05) in treatment T1, T2 and T3  compared to T0 control. The treatment T2 (2.0 mM glutathione) had the highest post-thaw sperm progressive motility, live sperm, HOS  response sperm percentage and reduced sperm abnormalities compared to other three groups. It was concluded that glutathione at  2.0 mM concentration significantly (p <0.05) improved post-thaw semen quality of buffalo bulls in comparison to control and glutathione  concentrations of 0.5 mM and 5.0 mM in Tris extender. 

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Published

2022-01-10

Issue

Vol. 18 No. 1 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan- Mar) 2022

Section

Articles

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How to Cite

Kumar, .K., & Gaur, M. (2022). Glutathione as a Semen Additive to Improve Surti Buffalo (Bubalus bubalis) Bull Semen Cryopreservation. Indian Journal of Veterinary Sciences and Biotechnology, 18(1), 116–118. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2274