Fertility Evaluation of Cryopreserved Osmanabadi Buck Semen at Field Level by Artificial Insemination of Estrus Synchronized Does

Authors

  • Vishalakshi Koli Department of Veterinary Gynecology and Obstetrics, Veterinary College, KVAFSU, Hebbal, Bangalore-560024, India
  • Bislere M. Ravindranath Department of Veterinary Gynecology and Obstetrics, Veterinary College, KVAFSU, Hebbal, Bangalore-560024, India
  • Annayappa Sahadev Department of Veterinary Gynecology and Obstetrics, Veterinary College, KVAFSU, Hebbal, Bangalore-560024, India
  • Guelal Sudha Department of Veterinary Gynecology and Obstetrics, Veterinary College, KVAFSU, Hebbal, Bangalore-560024, India
  • Gurubasayya P. Kalmath Department of Veterinary Physiology College, KVAFSU, Hebbal, Bangalore-560024,
  • Gudepalya J. Renukaradhya Department of Veterinary Gynecology and Obstetrics, Veterinary College, KVAFSU, Hebbal, Bangalore-560024, India

Keywords:

Artificial insemination, Conception rate, Estrus synchronization, Fertility, Ultrasonography

Abstract

Reproductive technologies like estrus synchronization and artificial insemination play an important role in enhancing goats'  reproductive efficiency and productivity. The present study was carried out to evaluate the fertility of cryopreserved Osmanabadi buck  (5) semen through AI on 100 multiparous synchronized does. Does were selected after thorough clinico-gynecological examination  and ultrasonographic evaluation for their reproductive status. From each of the selected bucks, five cryopreserved semen straws  each from four ejaculates were prepared and used for inseminating the estrus synchronized does. The does were subjected to estrus  synchronization protocol using an intra-vaginal sponge impregnated with 350 mg of natural progestagen for 7 days (Avikesil-S), followed  by intramuscular injection of 125 μg, Cloprostenol sodium (Estrumate) on the day of sponge withdrawal. Fixed time insemination was  performed 48-55 hours following sponge removal with simultaneous administration of 4 μg Buserelin acetate (Receptal). Pregnancy  was confirmed using ultrasonography at 30-35 days of insemination. The conception rate varied non-significantly from 35.00 ± 9.57 to  45.00 ± 5.77 % among five bucks, with an average of 40.00 ± 3.40 %.  

Downloads

Download data is not yet available.

References

Arora, C. L. and Garg, R. C. (1998). Sheep Production and Breeding. International Book Distribution Company, Lucknow, India. Arrebola, F., Perez-Marin, C. C. and Santiago-Moreno, J. (2010). Limitation of seasonality in reproductive parameters of Mediterranean bucks using photoperiod treatment. Small Ruminant Research, 89(1), 31-35.

Ankkul, N., Suwimonteerabutr, J., Tharasanit, T., Khunmanee, S., Diloksumpan, P., Berg, D. K. and Techakumphu, M. (2014). Sperm distribution and fertilization after unilateral and bilateral laparoscopic artificial insemination with frozen

thawed goat semen. Theriogenology, 82(8), 1137-1144. Batista, M., Nino, T., Alamo, D., Castro, N., Santana, M., Gonzalez, F., Cabrera, F. and Gracia, A. (2009). Successful artificial insemination using semen frozen and stored by an ultrafreezer in the Majorera goat breed. Theriogenology, 71, 1307-1315. Dorado, J., Rodriguez, I. and Hidalgo, M. (2007). Cryopreservation of goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates after artificial insemination. Theriogenology, 68, 168-177. Gibbons, A. E., Fernandez, J., Bruno-Galarraga, M. M., Spinelli, M. V. and Cueto, M. I. (2019). Technical recommendations for artificial insemination in sheep. Animal Reproduction, 16(4), 803-809.

Gacitua, H. and Arav, A. (2005). Successful pregnancies with directional freezing of large volume buck semen. Theriogenology, 63, 931-938.

Leethongdee, S. and Ponglowhapan, S. (2014). Artificial insemination in goats: An update. Thai Journal of Veterinary Medicine, .44(1), 73-77.

Martemucci, G. and Alessandro, A. G. (2011). Induction/ synchronization of oestrus and ovulation in dairy goats with different short term treatments and fixed time intrauterine or exo-cervical insemination system. Animal Reproduction Science, 126(3-4), 187-194.

Meza, C. A. and Ross, T.T. (2000). Factors affecting fertility and prolificacy of dairy goats inseminated with frozen-thawed semen. In: Proceedings of the 7th International Conference on Goats. Edt. Dubeuf, J. P., Institute de l’ Elevage (Idele): Tours, France., pp. 476-478.

Nunes, J. F. and Salgueiro, C. C. M. (2011). Strategies to improve the reproductive efficiency of goats in Brazil. Small Ruminant Research, 98(1-3), 176-184.

Paulenz, H., Soderquist, L., Adnoy, T., Soltun, K., Saether, P.A., Fjellsoy, K.R. and Berg, K.A. (2005). Effect of cervical and vaginal insemination with liquid semen stored at room temperature on fertility of goats. Animal Reproduction Science, 86(1-2), 109-117.

Purdy, P. H. (2006). A review on goat sperm cryopreservation. Small Ruminant Research, 63, 215-225.

Ritar, A. J., Ball, P. D. and O'may, P. J. (1990). Artificial insemination of Cashmere goats: effects on fertility and fecundity of intra-vaginal treatment, method and time of insemination, semen freezing process, number of motile spermatozoa and age of females. Reproduction Fertility and Development, 2(4), 377-384.

Singh, M.P., Sinha, A.K. and Singh, B.K. (1995). Effect of cryoprotectants on certain seminal attributes and on the fertility of buck spermatozoa. Theriogenology, 43, 1047-1053.

Downloads

Published

2022-01-10

How to Cite

Koli, V., Ravindranath, B.M., Sahadev, A., Sudha, G., Kalmath, G.P., & Renukaradhya, G.J. (2022). Fertility Evaluation of Cryopreserved Osmanabadi Buck Semen at Field Level by Artificial Insemination of Estrus Synchronized Does . Indian Journal of Veterinary Sciences and Biotechnology, 18(1), 104–106. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2268