Ovum Pick-Up and In Vitro Embryo Production from Stimulated Vs Non-Stimulated Buffaloes

Authors

  • Sanket P. Patil Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Sciences and Animal Husbandry, Kamdhenu University, Anand-388 001, Gujarat, India
  • Kamlesh K. Hadiya Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Sciences and Animal Husbandry, Kamdhenu University, Anand-388 001, Gujarat, India
  • Siddhartha S. Layek National Dairy Development Board, Anand-388 001, Gujarat, India,
  • Sanjay Gorani National Dairy Development Board, Anand-388 001, Gujarat, India
  • Sudharson Raj Sabarmati Ashram Gaushala, Bidaj, Kheda-387 120, Gujarat, India
  • Kuppusamy Karuppanasamy National Dairy Development Board, Anand-388 001, Gujarat, India
  • Arjun J. Dhami Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Sciences and Animal Husbandry, Kamdhenu University, Anand-388 001, Gujarat, India
  • Rajesh Onkarnath Gupta National Dairy Development Board, Anand-388 001, Gujarat, India

Keywords:

Buffalo, CIDR, Embryo production, FSH, OPU-IVEP, Ovarian stimulation

Abstract

The effect of ovarian stimulation prior to ovum pick-up (OPU) is yet to be fully assessed in buffaloes. Hence the present study was designed  to assess the effect of different ovarian stimulation regimes before OPU on in vitro embryo production (IVEP) in buffaloes. Nine selected  donor buffaloes were equally and randomly allocated to two stimulation protocols (SP1 and SP2) and a non-stimulated control. In the  SP1 group, an intravaginal progesterone device (CIDR) was inserted along with Estradiol Benzoate (2.0 mg, im) on a random day of the  estrous cycle (Day 0). In SP2 group, two PGF2α injections were administered im 11 days apart, followed by 10 µg GnRH im on day 14.  In SP1 on days 4 and 5, and in SP2 on days 16 and 17, buffaloes were administered a total of FSH 200 mg im, divided in four tapering  doses at 12 hours interval (57, 57, 43, and 43 mg). In the control group, OPU sessions were performed on day 10 of PGF2α induced estrus,  while OPU sessions were carried out after 52 hours of coasting period in SP1 and SP2 groups. Two OPUs were carried out on each donor  across experimental groups. Means of different groups were compared using one-way ANOVA using a general linear model. Although  a mean number of follicles aspirated per buffalo remained similar across all groups (22.0 ± 6.9 vs. 22.0 ± 6.0, 21.2 ± 5.8; p = 0.99), a mean  number of oocytes recovered/buffalo/OPU (14.8 ± 7.8 vs. 11.5 ± 3.4, 7.8 ± 2.3; p = 0.63) and oocyte recovery rate (59.2 ± 13.2 vs. 54.5 ±  4.8, 36.7 ± 3.4%; p = 0.17) was higher in the control group compared to both the stimulated groups. Attributing to ovarian stimulation in  both the stimulated groups, the cleavage rate (39.2 ± 10.1 vs. 66.3 ± 7.8, 68.9 ± 10.1%; p = 0.07) and blastocyst rate (8.7 ± 5.3 vs. 32.1 ± 8.9,  17.3 ± 7.1%; p = 0.10) were higher compared to the control group. The highest embryos/OPU (1.3 ± 0.8, 3.3 ± 1.0, 1.5 ± 0.6; P = 0.23) were  achieved in SP1 group as compared to Control and SP2 groups. The results indicate that ovarian stimulation increases oocyte quality,  especially when stimulated in the presence of exogenous luteal support, translating into better and cost-efficient IVEP in buffaloes. 

Downloads

Download data is not yet available.

References

Abreu, F.M., da Silva, M.C., Cruppe, L.H., Mussard, M.L., Bridges, G.A., Harstine, B.R., & Day, M.L. (2018). Role of progesterone concentrations during early follicular development in beef cattle: I. Characteristics of LH secretion and oocyte quality. Animal Reproduction Science, 196, 59-68.

Alapati, A., Kapa, S.R., Jeepalyam, S., Rangappa, S.M.P., &Yemireddy, K.R. (2010). Development of the body condition score system in Murrah buffaloes: validation through ultrasonic assessment of body fat reserves. Journal of Veterinary Science, 11(1), 1-8.

Baruselli, P.S., Soares, J.G., Bayeux, B.M., Silva, J.C.B., Mingoti, R.D., & Carvalho, N.A.T. (2018). Assisted reproductive technologies (ART) in water buffaloes. Animal Reproduction, 15, 971-983.

Campanile, G., Baruselli, P.S., Neglia, G., Vecchio, D., Gasparrini, B., Gimenes, L.U., & Michael, J.D. (2010). Ovarian function in the buffalo and implications for embryo development and assisted reproduction. Animal Reproduction Science, 121(1-2), 1-11.

Carvalho, J.G.S., Carvalho, N.A.T., Bayeux, B.M., Watanabe, Y.F., Watanabe, O.Y., Mingoti, R.D., & Baruselli, P.S. (2019). Super stimulation prior to the ovum pick-up improves the in vitro embryo production in nulliparous, primiparous and multiparous buffalo (Bubalus bubalis) donors. Theriogenology, 138, 164-168.

Cavalieri, J. (2018). Effect of treatment of Bos indicus heifers with progesterone 0, 3 and 6 days after follicular aspiration on follicular dynamics and the timing of estrus and ovulation. Animal Reproduction Science, 193, 9-18.

Chaubal, S.A., Ferre, L.B., Molina, J.A., Faber, D.C., Bols, P.E.J., Rezamand, P., & Yang, X. (2007). Hormonal treatments for increasing the oocyte and embryo production in an OPU–IVP system. Theriogenology, 67(4), 719-728.

Danell, B. (1987). Oestrus behaviour, ovarian morphology and cyclical variation in follicular system and endocrine pattern. PG Thesis. Swedish Univ. of Agricultural Sciences, Stockholm, Sweden.

Drost, M. (2007). Bubaline versus bovine reproduction. Theriogenology, 68,447-449.

Hafez E.S.E., & Hafez, B. (2000). Follicular atresia. In: Reproduction in Farm Animals, p 74-75.

Honparkhe, M., Gandotra, V., Matharoo, J., Ghuman, S., Dadarwal, D., & Singh, J. (2014). Synchronization of follicular wave emergence following ultrasound-guided transvaginal follicle ablation or estradiol-17β administration in water buffalo (Bubalus bubalis). Animal Reproduction Science, 146, 5-14.

Lamb, J.D., Zamah, A.M., Shen, S., McCulloch, C., Cedars, M.I., & Rosen, M.P. (2010). Follicular fluid steroid hormone levels are associated with fertilization outcome after intracytoplasmic sperm injection. Fertility and Sterility, 94(3), 952-957.

Lonergan, P., Monaghan, P., Rizos, D., Boland, M.P., & Gordon, I. (1994). Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization, and culture in vitro. Molecular Reproduction and Development, 37(1), 48-53.

Marin, D.F.D., Souza, E.B., Brito, V.C., Nascimento, C.V., Ramos, A.S., Rolim Filho, S.T., Costa, N.N., Cordeiro, M.S., Santos, S.S.D., &

Ohashi, O.M. (2019). In vitro embryo production in buffaloes: From the laboratory to the farm. Animal Reproduction Science, 16, 260-266.

Neglia, G., Gasparrini, B., Caracciolo di Brienza, V., Di Palo, R., &Zicarelli, L. (2003). First pregnancies to term after transfer of buffalo vitrified embryos entirely produced in vitro. Veterinary Research Communication, 28, 233-326.

Palta, P., Bansal, N., Prakash, B.S., Manik, R.S., & Madan, M.L. (1998). Endocrinological observation of atresia in individual buffalo ovarian follicles. Indian Journal of Animal Sciences, 68, 444-447.

Patel, M. (2020). Embryo production and pregnancy rates from OPU-IVEP using conventional and sexed semen in cattle. MV Sc Thesis, Anand Agricultural Univ., Anand, Gujarat, India.

Presicce, G.A., Jiang, S., Simkin, M., Zhang, L., Looney, C.R., &Godke, R.A. (1997). Age and hormonal dependence of acquisition of oocyte competence for embryogenesis in prepubertal calves. Biology of Reproduction, 56,386-392.

Sagheer, M., Ullah, F., Arshad, U., Saleem, M., Nawaz, M., Sarwar, Z., & Ahmad, N. (2020). Effect of photoperiodicity and methods of follicular wave emergence on follicle turn-over, recovery and quality of oocytes, and early in-vitro developmental competence of embryos using ovum pick-up in Nili-Ravi buffaloes: Preliminary evidence. Theriogenology, 157, 508-516.

Santos, S.S.D., Ferreira, M.A.P., Sampaio, R.V., Costa, N.N., Santos, D.C.N., Santana, P.P.B., & Ohashi, O.M. (2013). Evaluation of apoptosis as a mechanism of follicular cell atresia in the ovaries of cattle (Bos indicus) and buffalo (Bubalus bubalis) fetuses. Animal Reproduction,10(1), 55-61.

Seneda, M.M., Esper, C.R., Garcia, J.M., de Oliveira, J.A., &Vantini, R. (2001). Relationship between follicle size and ultrasound guided transvaginal oocyte recovery. Animal Reproduction Science, 67(1-2), 37-43.

Thanh, V.T.K., & Orskov, E.R. (2017). Protein digestion and metabolism in buffalo. In: Presicce GA, editor. The Buffalo (Bubalus bubalis): Production and Research. Bentham Science Publishers Ltd, ARSIAL Regione Lazio, Rome, Italy, p. 180-195.

Viana, J.H.M., de Almeida Camargo, L.S., de Moraes Ferreira, A., De Sa, W.F., de Carvalho Fernandes, C.A., & Junior, A.D.P.M. (2004). Short intervals between ultrasonographically guided follicle aspiration improve oocyte quality but do not prevent establishment of dominant follicles in the Gir breed (Bos indicus) of cattle. Animal Reproduction Science, 84(1-2), 1-12.

Vieira, L.M., Rodrigues, C.A., Castor Neto, A., Guerreiro, B.M., Silveira, C.R.A., Moreira, R.J.C., Sá Filho, M.F., Bó, G.A., Mapletoft, R.J., &Baruselli, P.S. (2014). Super-stimulation prior to the ovum pick-up to improve in vitro embryo production in lactating and non-lactating Holstein cows. Theriogenology, 82, 318-324.

Vieira, L.M., Rodrigues, C.A., Netto, A.C., Guerreiro, B.M., Silveira, C.R.A., Freitas, B.G., &Baruselli, P.S. (2015). Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle. Theriogenology, 85(5), 877-886.

Downloads

Published

2022-01-10

Issue

Vol. 18 No. 1 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan- Mar) 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Patil, S.P., Hadiya, K.K., Layek, S.S., Gorani, S., Raj, S., Karuppanasamy, K., … Gupta, R.O. (2022). Ovum Pick-Up and In Vitro Embryo Production from Stimulated Vs Non-Stimulated Buffaloes . Indian Journal of Veterinary Sciences and Biotechnology, 18(1), 95–100. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2258