Clinico-Haemato-Biochemical Alterations and Therapeutic Management of Bovine Theileriosis
Keywords:
Buparvaquone, Cattle, Clinical signs, Giemsa staining, Haemato-biochemical assay, Theileriosis, Therapeutic trialsAbstract
Theileriosis is an economically important haemoprotozoan disease of cattle caused by Theileria spp. It causes production losses and mortality in dairy industry. A total of 135 cattle suspected of theileriosis were screened based on clinical signs and blood smear examination (Giemsa’s stain). Out of them, 64 animals were diagnosed with theileriosis. Twenty-four cattle out of them were selected and divided into four equal groups, viz., B, C, D, and E, each comprised of six animals for four different treatment protocols to evaluate their therapeutic efficacy, while group A of six healthy cattle served as control. The most common clinical findings of theileriosis infected cattle (n = 24) in descending order were anorexia (100%), dullness and depression, decreased milk production, weakness, salivation, enlarged pre-scapular lymph nodes, pale conjunctival mucous membrane, presence of ticks over the body, emaciation, nasal discharge, cessation of rumination, lacrimation, diarrhea and sternal recumbency (8.33%). The mean rectal temperature, heart rate, and respiratory rate were 104.5 ± 0.33° F, 79.5 ± 2.85/min and 42.83 ± 2.67/min, respectively. The haematological findings revealed decreased values of haemoglobin, packed cell volume, total erythrocytes count (TEC), lymphocytes, and mean corpuscular hemoglobin concentration (MCHC), and increased values of neutrophils, eosinophils, Mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH) in theileriosis infected cattle as compared to healthy cattle. Biochemical findings revealed decreased values of total protein and albumin while increased total bilirubin, Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), and Gamma-glutamyl transferase (GGT) in theileriosis-infected cattle. These altered haemato-biochemical profiles were found to be normalized by the 14th day in different treatment groups. The therapeutic efficacy of Buparvaquone 2 dose (Group B) and Buparvaquone plus Oxytetracycline LA (Group D) was better (100.00%) than the Arteether (Group C, 66.66%) and Arteether plus Oxytetracycline LA (Group E, 83.33%) in theileriosis infected cattle, hence may be advocated to the field veterinarians for effective treatment of theileriosis in cattle.
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Abubakar, A.S., Hussein, A.M., Abdelsalam, M.A., Salih, D.A., Samia, H.A., HalaElrayah., & Sara, A.M. (2019). Alterations of hematological and biochemical profile of calves infected naturally with tropical theileriosis. American Journal of Biomedical Science Research, 5(3), 218-223
Acharya, A.P., Panda, S.K. & Prusty, B.K. (2017). Diagnosis and confirmation of Theileria annulata infection in cattle in Odisha, Indian Journal of Entomology and Zoology Studies, 5(4), 1543- 1546.
Aulakh, G.S., & Singla, L.D. (2006). Clinico-haemato-biochemical observations on bovines naturally infected with Theileria annulata. Journal of Veterinary Parasitology, 20(1), 49-52.
Brahmbhatt, N.N., Kumar, B., Thakre, B.J., Parmar, V.L., Choravada, D.R., & Patel, J.A. (2019). Clinico-hematological study of
theileriosis in Gir and Jaffrabadi calves. The Indian Journal of Veterinary Sciences and Biotechnology, 14(04), 36-39. Durrani, A.Z., & Kamal, N. (2008). Identification of ticks and detection of blood protozoa in Friesian cattle by polmerase chain reaction test and estimation of blood parameters in district Kasur, Pakistan. Tropical Animal Health and Production, 40(6), 441-447.
Forsyth, L.M.G., Jackson, L.A., Wilkie, G., Sanderson, A., Brown, C.G.D., & Preston, P.M. (1997). Bovine cells infected in vivo with Theileria annulata express CD11b, the C3bi complement receptor. Veterinary Research Communications, 21(4), 249-263.
Gachohi, J., Skilton, R., Hansen, F., Ngumi, P., & Kitala, P. (2012). Epidemiology of East Coast fever (Theileria parva infection) in Kenya: Past, present and the future. Parasites Vectors, 5, 194.
Goyal, P. (2018). Sex related hospital prevalence of Theileria annulata infection confirmed by nested polymerase chain reaction in cattle-calves in Bikaner District of Rajasthan. International Journal of Livestock Research, 8(5), 150-153.
Goud, K., Vijayakumar, K., & Davis, K.J. (2021). Haemato-biochemical studies of Theileria orientalis infection in cross bred dairy cattle. Journal Veterinary Animal Science, 52(1), 42-47.
Kachhawa, J.P., Kumar, S., Sharma, A., Singh, A.P., & Ahuja, A. (2016). Studies on alterations of clinical and hemato-biochemical parameters before and after treatment in calves naturally infected with theileriosis. Veterinary World, 9(12), 1381.
Kasozi, K.I., Matovu, E., Tayebwa, D.S., Natuhwera, J., Mugezi, I., & Mahero, M. (2014). Epidemiology of increasing hemo parasite burden in Ugandan cattle. Open Journal of Veterinary Medicine, 4(10), 220.
Khan, A., Ashfaq, K., Ud Din, I., ulHaq, R., Jamil, M., Ullah, B., ... & Ullah, F. (2017). Bovine theileriosis: Prevalence, estimation of hematological profile and chemotherapy in cattle in Dera Ismail Khan, Khyber Pakhtunkhwa Province, Pakistan. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 32(1), 8-17.
Khan, I.A., Khan, A., Hussain, A., Riaz, A., & Aziz, A. (2011). Hemato biochemical alterations in crossbred cattle affected with bovine theileriosis in semi-arid zone. Pakistan Veterinary Journal, 31(2), 137-140.
Khawale, T.S., Siddiqui, M.F., Borikar, S.T., Sakhare, M.P., & Shafi, T. (2019). Efficacy of arteether against theileriosis in cattle. Journal of Animal Research, 9(6), 883-888.
Kohli, S., Atheya, U.K., Srivastava, S.K., Banerjee, P.S., & Garg, R. (2014). Outbreak of theileriosis and anaplasmosis in herd of Holstein crossbred cows of Dehradun district of Uttranchal, India: A Himalyan region. International Journal of Livestock Production, 5(1), 6-9.
Kumar, A., Singh, M.K., Singh, G.D., Kumari, N., & Kala, S. (2018). Bovine theileriosis and its therapeutic management in crossroad cows- A clinical study in 3 cases. Journal of Agricultural Search, 6(1), 119-121.
Kumar, B., Mondal, D.B., Jithin, M.V., & Raguvaran, R. (2016). Therapeutic management of theileriosis - A clinical study of 5 bovine calves. Intas Polivet, 17(1), 92-93.
Nagar, J.K., Gurjar, T., Mali, M.M., Bargujar, J., Meena, O., & Kumar, A. (2019). Therapeutic management of theileriosis in bovines. Journal of Veterinary Parasitology, 4(1), 12-14.
Naik, B. S., Maiti, S. K., &Raghuvanshi, P. D. S. (2016). Prevalence of tropical theileriosis in cattle in Chhattisgarh State. Journal of Animal Research, 6(6), 1043-1045.
Ngumi, P.N., Young, A.S., Lampard, D., Mining, S.K., Ndungu, S.G., Lesan, A.C., & Kariuki, D.P. (1992). Further evaluation of the use
of buparvaquone in the infection and treatment method of immunizing cattle against Theileria parva derived from African buffalo (Syncerus caffer). Veterinary Parasitology, 43(1-2), 15-24.
Pandey, V., Nigam Rajesh, Bachan, R., Sudan, Vikrant., Jaiswal, A.K., Shanker, D., & Yadav, Brijesh. (2017). Oxidative and haemato biochemical alterations in theileriosis affected cattle from semi arid endemic areas of India. Indian Journal of Animal Sciences, 87(7), 846-850.
Radostits, O. M., Gay, C. C., Hinchcliff, K. W., & Constable, P. D. (2008). Anaplasmosis. In: Veterinary Medicine: A textbook of the diseases of cattle, horses, sheep, pigs, and goats. 10th edn., Saunders, Elsevier, pp. 1455-1459.
Sandhu, G.S., Grewal, A.S., Singh, A., Kondal, J.K., Singh, J., & Brar, R.S. (1998). Haematological and biochemical studies on experimental Theileria annulata infection in crossbred calves. Veterinary Research Communications, 22(5), 347-
Saravanan, M., Ranjithkumar, M., Bapu Prasanth, N., Yogeshpriya, S., Jayalakshmi, K., & Kannan, K. (2017). Clinical, hematological changes and therapeutic efficacy of buparvaquone with oxytetracycline against the natural infection of Theileria annulata in cattle. International Journal of Livestock Research, 7(10), 128-133.
Siddiqui, M.F., Waghmare, S.P., Mode, S.G., Rekhate, D.H., Kolte, S.W., Hajare, S.W., & Ali, S.S. (2017). Phyto-pharmacological study and therapeutic efficacy of Calotropisprocera (flower) against theileriosis in cattle. Journal of Animal Research, 7(4), 757-762.
Snedecor, G.W., & Cochran, W.G. (1994). Statistical Methods. 8th edn. Iowa State University Press, Ames, Iowa, USA.
Tuli, A., Singla, L.D., Sharma, A., Bal, M.S., Filia, G., & Kaur, P. (2015). Molecular epidemiology, risk factors and haematochemical alterations induced by Theileria annulata in bovines of Punjab (India). Acta Parasitologica, 60(3), 378-390.
Ugalmugle, S.S., Jayraw, A.K., & Gatne, M.L. (2010). Prevalence and clinical pathology of bovine tropical theileriosis in crossbred population of Ahmednagar district of Maharashtra. Journal of Veterinary Parasitology, 24(2), 141-145.
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