Molecular Detection, Phylogenetic Analysis and Differentiation of Fowl Adenovirus from Chickens with Inclusion Body Hepatitis in Haryana

Authors

  • Aman Kumar Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
  • Koushlesh Ranjan Department of Veterinary Physiology and Biochemistry, SVP University of Agriculture and Technology, Meerut, Uttar Pradesh, India
  • Minakshi Prasad Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
  • Nand Kumar Mahajan Department of Veterinary Public health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
  • Sushila Maan Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India

Keywords:

Fowl Adenovirus, Inclusion Body Hepatitis, Polymerase Chain Reaction, Restriction enzyme

Abstract

Inclusion Body Hepatitis (IBH) is responsible for huge economic losses to the poultry industry and is caused by Fowl adenoviruses  (FAdV). A total of thirty pooled poultry liver homogenates from thirty different poultry farms suspected of IBH have been processed for  the PCR-based detection and chicken embryo liver cell culture-based virus isolation. Out of 30 pooled samples, 18 were found positive  in PCR; however, only 9 chicken embryo liver cell culture samples having CPE found positive in PCR. The nucleic acid obtained from  FAdV specific Polymerase Chain Reaction (PCR) were further genotyped by in-silico restriction enzyme analysis (REA) using enzymes  BsiWI, KpnI, and HpaI. Upon phylogenetic analysis, all the nine positive samples of FAdVs clustered in distinct clads of FAdV serotypes  8a, 8b, and 11, which is in correlation with the REA pattern. Thus, nucleic acid sequencing followed by phylogenetic analysis revealed  the presence of FAdV-D (serotype 11) and FAdV-E (serotype 8a and 8b) in broiler chickens. 

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Published

2022-09-19

Issue

Vol. 18 No. 2 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Apr- Jun) 2022

Section

Articles

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kumar, A., Ranjan, K., Prasad, M., Mahajan, N.K., & Maan, S. (2022). Molecular Detection, Phylogenetic Analysis and Differentiation of Fowl Adenovirus from Chickens with Inclusion Body Hepatitis in Haryana. Indian Journal of Veterinary Sciences and Biotechnology, 18(2), 113–117. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2180