Association Study of Fecundity Gene BMP 15 with Prolificacy in Surti Goats under Farm and Field Condition of South Gujarat Region
Keywords:
BMP15, Fecundity gene, Polymorphism, Prolificacy, Surti goatAbstract
The Surti is a dual-purpose goat breed of Gujarat. The bone morphogenetic protein 15 (BMP15), a gene of transforming growth factor-beta (TGF-β) superfamily ligands, plays a role in ovulation and litter size. Mutation in the Exon-2 region of BMP15 gene with base size 575 bp increases litter size. Based on the known mutation information in goat and sheep PCR primers were designed to screen polymorphism in total 100 Surti goats, 50 Surti goats from University farm and 50 Surti goats from field units of the Southern part of Gujarat. Two polymorphic sites were found during the study, one at 500 bp and another at 400 bp. Based on this polymorphism, three genotypes were denoted as AA, AB, and AC for a wild or non-polymorphic region of 575 bp, polymorphic region at 500 bp, and polymorphic region at 400 bp, respectively. The AA, AB, and AC genotypic frequency was 0.58, 0.29, and 0.13 with average kidding rate 3.01 ± 0.09, 4.65 ± 0.32 and 2.84 ± 0.24, respectively. Polymorphic region of BMP15 gene at 500 bp (AB genotype) might be used as marker genotype for higher kidding rate at early age selection of female Surti goat.
Downloads
References
Arnyasi, M., Zsolnai, A., Komlosi, I., Fesus, L., & Javor, A. (2004). Case study of a Hungarian breeding program using imported Booroola rams. Archiv fur Tierzucht, 47, 359-366.
Bayan, J., Kharadi, V., Ramani, U., Janmeda, M., Tyagi, K., Dangar, N., & Pandya, G. (2018). Genetic polymorphism of growth hormone gene exon-4 in Surti and Mehsani goats by PCR-RFLP. The Indian Journal of Veterinary Sciences and Biotechnology, 14(1),
-33.
Chu, M.X., Jiao, C.L., He, Y.Q., Wang, J.Y., Liu, Z.H., & Chen, G.H. (2007). Association between PCR-SSCP of bone morphogenetic protein15 gene and prolificacy in Jining Grey goats. Animal Biotechnology, 18, 263-274.
Cui, H.X., Zhao, S.M., Cheng, M.L., Guo, L., Ye, R.Q., Liu, W.Q., & Gao, S.Z. (2009). Cloning and expression levels of genes relating to the ovulation rate of the Yunling black goat. Biology of Reproduction, 80, 219-226.
Dangar, N.S., Pandya, G.M., Ramani, U.V., Padheriya, Y.D., Sangma, T., Patel S.S., & Devkatte, S. (2019), Study of fecundity gene GDF9 with prolificacy in Surti goats under farm and field condition. The Indian Journal of Veterinary Sciences and Biotechnology,
(4), 17-20.
Dangar, N.S., Pandya, G.M., Ramani, U.V., Patel S.S., & Ansari, F. (2021). Study on genetic polymorphism in genes related to prolificacy and foetal growth using PCR-RFLP in Surti goats. The Indian Journal of Veterinary Sciences and Biotechnology, 17(4), 31-35.
Fabre, S., Pierre, A., Mulsant, P., Bodin, L., Di Pasquale, E., Persani, L., Monget, P., & Monniaux, D. (2006). Regulation of ovulation rate in mammals: contribution of sheep genetic models. Reproduction Biology and Endocrinology, 4, 20.
Godara, A., Gahlot, G.C., Ashraf, M. & Ghorui, S.K. (2011). Polymorphism of growth hormone BMP15 gene in Marwari goats. Indian Journal of Small Ruminants, 18, 32-36.
Hua, G.H., Chen, S.L., Ai, J.T. & Yang, L.G. (2008). None of polymorphism of ovine fecundity major genes and FecB FecX was tested in goat. Animal Reproduction Science, 108, 279-286.
Jemmali, B., Bedhiaf, S. & Djemali, M. (2012). In the search of genetic polymorphism of bone morphogenetic protein with PCR-RFLP in sheep. Journal of Animal Science Advance, 2, 268-273.
Kuralkar, S.V., Verma, N.K., Kharkar, K. & Kuralkar, P. (2013). Berari goats: characterization, management, performance and population status. Indian Journal of Animal Science, 83, 1292- 1298.
Mohammadi, G. (2016). Determination of FecX FecB and FecGH mutations in Iranian Arabic sheep. Basrah Journal of Veterinary Research, 15, 435-445.
Monteagudo, L.V., Ponz, R., Tejedor, M.T., Lavina, A., & Sierra, I. (2009). A 17bp deletion in the bone morphogenetic protein 15 (BMP15) gene is associated to increased prolificacy in the Rasa Aragonesa sheep breed. Animal Reproduction Science,
, 139-146.
Moore, R.K., Erickson, G.F. & Shimasaki, S. (2004). Are BMP-15 and GDF9 primary determinants of ovulation quota in mammals? Trends Endocrinology Metabolism, 15, 356–361.
Moore, R.K. & Shimasaki. (2005). Molecular biology and physiological role of the oocyte factor. BMP-15. Molecular Cellular Endocrinology, 234, 67-73.
Pandya, G.M., Ramani, U.V., Tyagi, K.K., Dangar, N.S., & Janmeda, M. (2020). Genetic polymorphism of GHR, LEP and MSTN genes in Surti Goats from Organized farm. The Indian Journal of
Veterinary Sciences and Biotechnology, 16(2,3&4), 21-24. Panhale, M.A., Kuralkar, S.V., Ali, S.S., Bankar, P.S., Kuralkar, P., & Deshmukh, S.G. (2018). Absence of polymorphism of fecundity genes (FecxB And FecxH) in Berari goats. Indian Journal of Small Ruminants, 24, 334-337.
Roy, J., Polley, S., De, S., Mukherjee, A., Batabyal, S., Pan, S., Brahma, B., Datta, T.K., & Goswami, S.L. (2011). Polymorphism of fecundity genes (FecB, FecX and FecG) in the Indian Bonpala sheep. Animal Biotechnology, 22, 151-162.
Sambrook, J., & Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual. 3 edn, Cold Spring Harbor Cold Rd, Spring Laboratory Press, New York, USA.
Sharma, L., Kuralkar, S.V., Ali, S.S., Bankar, P.S. & Kuralkar, P. (2016). Polymorphism study of BMPR-1B BMP-15 and genes in Berari goats. Indian Journal of Small Ruminants, 22, 230-233.
Shi, H., Bai, J., Niu, Z., Muniresha, F.L. & Jia, B. (2010). Study on candidate gene for fecundity traits in Xingjiang Celeblack sheep. African Journal of Biotechnology, 9, 8498-8505.
Shokrollahi, B. (2015). Investigation of gene BMP15 polymorphisms associated with twining in Markhoz goat. Biharean Biologist, 9, 1-4.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
