Microbiological Contamination of Retail Meat from Mizoram (India) with Special Reference to Molecular Detection and Multi-Drug Resistance of Escherichia coli

Authors

  • Mujesh Debbarma Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram: 796015, India
  • Devajani Deka Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram: 796015, India
  • Thingujam ChaaTolenkhomba Department of Animal Genetics and Breeding, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram: 796015, India
  • Justus B. Rajesh Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram: 796015, India

Keywords:

E. coli, Microbial Contamination, Molecular Detection, Multidrug Resistance, Retail meat

Abstract

Food articles serve as a common vehicle for transmitting many pathogenic and non-pathogenic microorganisms to humans, and many of  these organisms may have developed multidrug resistance. Meat may act as a vehicle for transferring multi-drug-resistant organisms to  the consumers. In the present study, a total of 180 (retail beef, N = 90 and chicken meat, N = 90) samples were collected from 3 districts of  Mizoram and analyzed for the level of microbial contamination, isolation, and molecular detection of Escherichia coli (E. coli) and its drug  resistance pattern. Unacceptable levels of total viable count (TVC) and Escherichia coli count (ECC) were recorded in retail beef and chicken.  On PCR assay-based confirmation, E. coli was detected in beef (83.33%) and chicken (80.00%). Significantly (p ≤0.01) lower prevalence of E.  coli was recorded in chicken meat from Champhai district (63.33%) than Aizawl (86.67%) and Kolasib (90.00%) districts. Resistance of E. coli strains to amoxicillin was highest with detection of MDR E. coli from beef (42.67%) and chicken (56.94%), indicating public health concerns.

Downloads

Download data is not yet available.

References

Abebe, E., Gugsa, G., & Ahmed, M. (2020). Review on major food borne zoonotic bacterial pathogens. Journal of Tropical Medicine, 2020, 4674235, https://doi.org/10.1155/2020/4674235

Ahmed, M.U.D., Sarwar, A., Najeeb, M.I., Nawaz, M., Anjum, A.A., Ali, M.A., & Mansur, N. (2013). Assessment of microbial load of raw meat at abattoirs and retail outlets. Journal of Animal and Plant Science, 23, 745-748.

Akbar, A., Sitara, U., Khan, S.A., Ali, I., Khan, M.I., Phadungchob, T., & Anal, A.K. (2014). Presence of Escherichia coli in poultry meat: A potential food safety threat. International Food Research Journal, 21, 941-945.

Bauer, A.W., Kirby, W.M.M., Sherris, J.C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized products single disc method. American Journal of Clinical Pathology, 45, 493-496.

Bradeeba, K., & Sivakumaar, P.K. (2013). Assessment of microbial quality of beef, mutton and pork and its environment in retail shops in Chidambaram, Tamil Nadu. International Journal of Plant, Animal and Environmental Science, 3, 9197.

Candrian, U., Furrer, B., Hofelein, C., Meyer, R., Jermini, M., & Lüthy, J.(1991).Detection of Escherichia coli and identification of enterotoxigenic strains by primer-directed enzymatic amplification of specific DNA sequences. The International Journal of Food Microbiology. 12, 339-51. DOI: 10.1016/0168- 1605(91)90148-i

Fawole, M.O., & Oso, BA (2001). Laboratory Manual of Microbiology, Edn Revised, Spectrum Books Ltd, Ibadan, Owerri, PP: 71-81. Heredia, N., & García, S. (2018). Animals as sources of food-borne pathogens: A review. Animal Nutrition, 4, 250-255.

Huges, FA, Adu- Gyamfi, A., & Appiah, V. (2015). Microbiological and parasitological quality of local beef retailed in Accra and radiation sensitivity of salmonella sp. International Journal of Current Microbiology and Applied Science, 4, 86-96.

ICMSF (1974). Sampling for microbiological analysis: Principles and specific applications. In: Microorganism in foods, Edn 2nd, Black Well Scientific Publication, Academic Press, New York, pp:102-118.

Kshirsagar, D. P., Sinha N., Brahmbhatt M. N., & Nayak J. B. (2014). Detection of potentially pathogenic E. coli O157H7 in buffalo meat samples by conventional and molecular methods. International Journal of Livestock Research., 4, 27-32.

Patyal, A., Gangil, R., Singh, P.K., Mathur, K.N., & Sudan,V. (2012). Bacteriological quality of market chicken meat in Jaipur city. Journal of Veterinary Public Health, 10, 45-48.

Quinn, P.J., Carter, M.E., Markey, B., & Carter, G.R. (2013). Clinical Veterinary Microbiology. Mosby International, Elsevier Limited, London, Edn 2nd, pp: 118-126.

Ramya, P., Reddy, E.T., Kumar, A.V., & Krishnaiah, N. (2015). A study on microbiological analysis of raw meat. International Journal of Institutional Pharmacy and Life Science, 5, 1-5.

Saikia, P., & Joshi, S.R. (2012). A Study on the occurrence of non-O157 Shiga toxin-producing Escherichia coli isolates in retail chicken meats marketed in North-East India. In the proceedings of the National Academy of Sciences, India, DOI: 10.1007/s 40011-

-0143-9.

Saud, B., Paudel, G., Khichaju, S., Bajracharya, D., Dhungana, G., Awasthi, M.S., & Shrestha, V. (2019). Multidrug resistant bacteria from raw meat of buffalo and chicken, Nepal. Veterinary Medicine International, 7960268, DOI: 10.1155/2019/7960268

Singh, V.K., Jain, U., Yadav, J.K., & Bist, B. (2014). Assessment of bacterial quality of raw meat samples (carabeef, chevon, pork and poultry) from retail meat outlets and local slaughterhouses of Agra Region, India. Journal Foodborne and Zoonotic Diseases, 2, 15-18.

Downloads

Published

2022-09-19

Issue

Vol. 18 No. 2 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Apr- Jun) 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Debbarma, M., Deka, D., ChaaTolenkhomba, T., & Rajesh, .J.B. (2022). Microbiological Contamination of Retail Meat from Mizoram (India) with Special Reference to Molecular Detection and Multi-Drug Resistance of Escherichia coli . Indian Journal of Veterinary Sciences and Biotechnology, 18(2), 32–35. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2151