Molecular Characterisation and Antimicrobial Resistance Patterns of Shiga Toxin Producing Escherichia coli Isolated from Farm Water Samples
DOI:
https://doi.org/10.48165/ijvsbt.18.4.15Keywords:
Antimicrobial resistance, Escherichia coli, Farm water samples, Shiga toxin producingAbstract
Shiga toxin-producing Escherichia coli (STEC) strains are considered the most common food-borne zoonotic pathogen and are highly pathogenic to humans in low infectious doses, causing food-borne diseases through consumption of contaminated water or food. Resistance against antibiotics by STEC is a big concern nowadays. Two hundred farm water samples (Cattle-40, Buffaloes-40, Sheep-30, Goat-20, Pig-20 and poultry-50) were collected aseptically from different livestock farms in and around Proddatur, Andhra Pradesh, India and Processed for E. coli isolation, identification with culture method and molecular characterization by PCR. E. coli was characterized as STEC with two genes i.e. stx1 and stx2. All the STEC isolates were subjected to an antibiotic sensitivity test by disc diffusion method against ten antibiotics. Results showed that out of 200 farm water samples, 196 were positive for E. coli with an overall prevalence of 98% (196/200) and 62.2% (122/196) for STEC by PCR. Antimicrobial susceptibility test by disc diffusion method against ten antibiotics revealed the higher resistance to Cepahlothin (100%) followed by Tetracyclin (98.4%), Ampicillin (96.7%), Streptomycin (95%), Sulphonamides (91.8%), Trimethoprim (84.4%), Kanamycin (34.4%), Chloromphenicol (17.2%), Colistin (9.0%) and least resistance to Gentamycin (4.9%).
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Ahmad, M.D., Hashmi, R.A., Anjum, A.A., Hanif, A. &Ratyal, R. H. (2009). Drinking water quality by the use of congored medium to differentiate between pathogenic and non pathogenicE. Coli at poultry farms. The Journal of Animal & Plant Sciences,19(2), 108-110.
Arora, S., Agarwal, R.K. &Bist, B. (2006). Comparision of ELISA and PCR vis-a-vis cultural methods from detecting Aeromonas spp. In foods of animal origin. Int. J.Food Microbiol, 106(2), 177-183.
Ateba, C.N. &Mbewe, M. (2011). Detection of E.coli O157H7 virulence genes in isolates from beef, pork, water, human, and animal species in the Northwest provinde, South Africa”: public health implications. Research in Microbiology, 162(3), 240-248.
Benedict, L., James., Marlene, E., Gutiierrez& Myriam. (2014). Prevalence of Escherichia coli O157:H7. Louisiana Agriculture. Bruyand, M., Mariani-Kurkdjian, P., Gouali, M., de Valk, H., King, L.A., Le Hello, S., et al., (2018). Hemolytic uremic syndrome due to Shiga toxin-producing Escherichia coli infection. Med. Mal. Infect, 48 (3), 167-174.
Colello, R., Etcheverría, A.I., Di Conza, J.A., Gutkind, G.O., Nora, L. &Padola. (2015). Antibiotic résistance and intégrons in Shiga toxin-producing Escherichia coli (STEC). Brazilian Journal of Microbiology, 46(1), 1-5.
Dweik, M., Stringer, R. C., Dastider, S. G., Wu, Y., Almasri, M., &Barizuddin, S. (2012). Specific and targeted detection of viable Escherichia coli O157:H7 using a sensitive and reusable impedance biosensor with dose and time response studies. Talanta, 94, 84-89.
El-Jakee, J.K., Mahmoud, R.M., Samy, A.A., El-Shabrawy, M.A., Effat, M.M. & Gad El-Said. W.A. (2012). Molecular Characterization of E. coli Isolated from Chicken, Cattle and Buffaloes. International
Journal of Microbiological Research, 3 (1), 64-74.
El-Shatoury, E.H., Azab El-Leithy, M., Abou-Zeid, M.A., El-Taweel, M.E. & El-Senousy, W.M. (2015). Antibiotic Susceptibility of Shiga Toxin Producing E. coli O157:H7 Isolated from Different Water Sources. The Open Conference Proceedings Journal, 6, 30-34.
Fashina, C.D., Babalola, G.O. &Osunde, M.O. (2018). Prevalence and Molecular Characterization of E. coli O157:H7 Isolated from Water Bodies in Ile-Ife and Environs. Journal of Bacteriology and Parasitology, 9(4), 1-5.
Kiranmayee, CH. &Krishnaiah, N. (2011). Detection of Shiga toxigenic Escherichia coli by PCR. Indian Veterinary Journal, 88(8), 19-20. Koutsoumanis, K., Allende, A., Alvarez-Ordonez, A., Bover-Cid, S., Chemaly, M., Davies, R., et al., (2020). Pathogenicity assessment of Shiga toxin-producing Escherichia coli (STEC) and the public health risk posed by contamination of food with STEC. EFSA J, 18 (1), 5967.
Kumar A, Taneja N, Bharti B. & Sharma M. (2014). Characterization of Shiga toxigenic Escherichia coli isolated from cases of diarrhea &haemolyticuraemic syndrome in North India. Indian Journal of Medical Research, 140, 778-784.
Makhado U.G., Foka, F.E.T., Tchatchouang, C.D.K., Ateba, C.N. &Manganyi, M.C. (2022). Detection of virulence gene of Shiga toxin-producing Escherichia coli (STEC) strains from animals with diarrhoea and water samples in the North-West Province, South Africa. Gene Reports, 27, 101617.
Manna, S. K., Brahmane, M. P., Manna, C., Batabyal1, K. & Das, R. (2006). Occurrence, virulence characteristics and antimicrobial resistance of Escherichia coli O157 in slaughtered cattle and diarrhoeic calves in West Bengal, India. Letters in Applied Microbiology, 43, 405–409.
Moore, J.E., Crothers, L.J.D., Millar, B.C. & Rooney, P.J. (2004). “Occurrence of verocytotoxin-producing Escherichia coli in dairy and meat processing environments”, A comment on: Journal. of Food Protecion,66(9), 1576-1580 (2003). In: Journal of Food Protection. 67(4): 636-637.
Ongor, H., Kalin, R. &Cetinkaya, B. (2007). Investigations of Escherichia coli O157 and some virulence genes in samples of meat and faeces from clinically healthy cattle in Turkey. The Veterinary Record, 161, 392-394.
Orth, D., Grif, K., Zimmerhackl, L. B. &Wurzner, R. (2009). Sorbitol fermenting Shiga toxin-producing Escherichia coli O157 in Austria. Wiener klinischeWochenschrift, 121, 108-112.
Padola, N.L., Sanz, M.E., Blanco, J.E., Blanco, M., Blanco, J., Etcheverria, A., Arroyo, G.H., Usera, M.A. & Parma, A.E. (2004). Serotypes and virulence genes of bovine Shiga-toxigenic E.coli (STEC) isolated from a feedlot in Argentina. Veterinary Microbiology, 100, 3-9.
Perera, A., Clarke C.M., Dykes G.A. &Fegan N. (2015). Characterization of Shiga toxigenic Escherichia coli O157 and non O157 isolates from ruminant feces in Malaysia. Biomed Research International, 2, 1-8.
Rao, K.V. (2015). Studies on the incidence of shiga toxin – producing Escherichia coli (STEC) in dairy farm animals. M.V.Sc. Thesis submitted to Sri P.V.Narasimha Rao Telangana Veterinary University.
Rao, T.S., Gill, J.P.S., Ravikumar, G.V.V.P.S. &Ghatak, S. (2011). Multi drug resistance patterns of Shiga toxin – producing Escherichia coli (STEC) and non – STEC isolates from meats, RTE meat foods, drinking water and human diarrhoeic samples of Punjab, India. Archives of Clinical Microbiology, 2(2:3), 1-12.
Shahi S. K., Singh V. K. & Kumar A. (2013). Detection of Escherichia coli and associated β-lactamases genes from diabetic foot ulcers by multiplex PCR and molecular modelling and docking of SHV-1, TEM-1, and OXA-1 β-lactamases with clindamycin and piperacillin-tazobactam. PLoS ONE,8, 1-5.
Walker, C. L., Applegate, J. A. & Black, R. E. (2012). Haemolyticuraemic syndrome as a sequela of diarrhoeal disease. Journal of Health Population and Nutrition, 30(3), 257-261.
Yilmaz, E.G. (2014). Sheep as an important source of E. coli O157/ O157:H7 in Turkey. Veterinary Microbiology, 172(3–4), 590–595.
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