Effect of Different Egg Yolk Levels in Tris Extender With and Without Aloe Vera and Onion Crude Extracts on Surti Buck Semen at Refrigerated Temperature

Authors

  • Dignesh K. Patel Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat-396 450, India
  • Chandrakant F. Chaudhari Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat-396 450, India.
  • Lalit C. Modi Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat-396 450, India.
  • Naresh F. Chaudhari Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat-396 450, India.
  • Mahesh M. Chaudhary Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat-396 450, India.
  • Kuldeep K. Tyagi Department of Animal Genetics & Breeding, College of Veterinary & Animal Sciences, Sardar Vallabhbhai Patel University of Agricultural and Technology, Meerut, UP-250110
  • Sunil V. Kunvar Department of Animal Nutrition, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat-396 450, India.

DOI:

https://doi.org/10.48165/ijvsbt.18.4.10

Keywords:

Aloe vera, Egg yolk, Onion, Semen quality, Surti buck

Abstract

This study aimed to develop a suitable Tris-citrate-fructose-egg yolk (Tris) based extender with Aloe vera or onion extract to preserve  Surti buck semen at refrigerated temperature. Semen ejaculates (n=96) collected by artificial vagina from eight bucks were split-diluted  in Tris extender with 0, 5, 10, 20% egg yolk concentrations. All these were then fractionated into three aliquiots each, one fracrtion kept  without further additives (EY0, EY5, EY10, EY20), while fractions 2 and 3 were an addition with crude filtrates of Aloe vera gel @ 10 µL  (EY0+A, EY5+A, EY10+A, EY20+A) or onion extract @ 10 µL (EY0+O, EY5+O, EY10+O, EY20+O) per 0.5 ml of the extended semen. These  samples (4x3 = 12 aliquots, each 0.5 ml) were then preserved at refrigerated temperature and evaluated at 12 hourly interval up to 48  h of storage for various sperm quality tests. Sperm motility was significantly lower (p<0.01) in all the subgroups with 0% egg yolk (EY0,  EY0+A, EY0+O) compared to 5 to 20% egg yolk at all intervals. The sperm motility at 48 h was significantly (p<0.01) lower in EY0 without  and with Aloe vera and onion (11.49 ± 2.54, 10.12 ± 2.45 and 6.58 ± 2.30 %) than EY5 without and with Aloe vera and Onion extract  (53.85 ± 1.41, 56.82 ± 1.20, 34.84 ± 2.25%), EY10 without and with Aloe vera and onion (55.83 ± 1.19, 57.76 ± 1.10, 35.37 ± 2.15%), EY20  without and with Aloe vera and onion extract (56.09 ± 1.32, 56.46 ± 1.26 and 41.06 ± 1.92%, respectively). The live sperm count was  significantly lower (p<0.01) in semen extended with 0% egg yolk as compared to 5, 10 and 20% egg yolk at 0, 24 and 48 hours. The  post-chilled abnormal sperm percentage increased non-significantly at 24 and 48 hours in all extender combinations, although the  effect of additives was non significant. It is concluded that adding egg yolk alone 5–20% or with Aloe vera extract @ 10 µL in semen  extender had a beneficial role in Surti buck semen preservation at refrigerated temperature. 

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Published

2022-09-15

Issue

Vol. 18 No. 4 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Sept- Oct) 2022

Section

Articles

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Patel, D.K., Chaudhari, .C.F., Modi, L.C., Chaudhari, N.F., Chaudhary, M.M., Tyagi, K.K., & Kunvar, S.V. (2022). Effect of Different Egg Yolk Levels in Tris Extender With and Without Aloe Vera and Onion Crude Extracts on Surti Buck Semen at Refrigerated Temperature . Indian Journal of Veterinary Sciences and Biotechnology, 18(4), 44–49. https://doi.org/10.48165/ijvsbt.18.4.10
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