Influence of Direct-Fed Microbials on In-Vitro Dry Matter Digestibility, Rumen Fermentation Parameters and its Economics in Adult Sheep
DOI:
https://doi.org/10.48165/ijvsbt.20.6.17Keywords:
Ammonia nitrogen, DFM, IVDMD, pH, Sheep, Rumen FermentationAbstract
The present study was aimed to find out the effect of direct fed microbials (DFM) on in-vitro dry matter digestibility (IVDMD), rumen fermentation parameters and its economics in 20 adult sheep. Based on the promising results from the laboratory-based in-vitro trials (49.55% IVDMD), the in-vivo study with a selected 2% level of DFM was further investigated. The sheep were arbitrarily split into two equal groups of ten each. The animals under T1 and T2 were fed compound concentrate mixture and roughage in conventional farm feeding as per ICAR (2013), additionally treatment group (T2) was supplemented with 2% DFM. The results for rumen fermentation pattern revealed that the average pH and TVFA values for T1 and T2 did not differ significantly. The experimental animals of DFM treatment group showed significantly (p<0.05) higher concentration of average total nitrogen (118.91 Vs. 100.71 mg/dL). The ammonia N, NPN concentration and TCA precipitable nitrogen concentration values were found to be non-significant (p>0.05) between two groups, while the ammonia N concentration was significantly (p<0.05) higher at 3 and 6 h post-feeding in both control and treatment group. The TCA precipitable nitrogen concentration values in rumen liquor neither differed significantly between groups nor at different time intervals post-feeding.
Downloads
References
BAHS (2022). Basic Animal Husbandry Statistics. Department of Animal Husbandry, Dairying and Fisheries, Government of India, New Delhi.
Biricik, H., & Turkmen, I.I. (2001). The effect of Saccharomyces cerevisiae on in-vitro rumen digestibilities of dry matter, organic matter and neutral detergent fibre of different forage: concentrate ratios in diets. Journal of the Faculty of Veterinary Medicine, 20, 29.
Blummel, M., Karsli, A., & Russell, J. R. (2003). Influence of diet on growth yields of rumen microorganisms in vitro and in vivo: Influence on growth yield of variable carbon fluxes to fermentation products. British Journal of Nutrition, 90, 625-634.
Carro, M.D., Lebzien, P., & Rohr, K. (1992). Influence of yeast culture on the in vitro fermentation (Rusitec) of diets containing variable portions of concentrates. Animal Feed Science and Technology, 37(3-4), 209-220.
Direkvandi, E., Mohammadabadi, T., & Salem, A.Z. (2020). Oral administration of lactate producing bacteria alone or combined with Saccharomyces cerevisiae and Megasphaera elsdenii on performance of fattening lambs. Journal of Applied Animal Research, 48(1), 235-243.
Elseed, F.A.M.A., & Abusamra, R.M.A. (2007). Effects of supplemental yeast (Saccharomyces cerevisiae) culture on NDF digestibility and rumen fermentation of forage sorghum hay in Nubian goat’s kids. Research Journal of Agriculture & Biological Sciences, 3(3), 133-137.
Getachew, G., Makkar, H.P.S, & Becker, K. (2002). Tropical browses: Contents of phenolic compounds, in vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production. Journal of Agricultural Science, 139, 341-352.
ICAR (2013). Nutrient Requirements of Sheep, Goat and Rabbit, Indian Council of Agricultural Research, Krishi Bhawan, New Delhi, India.
Lynch, H.A., & Martin, S.A. (2002). Effects of Saccharomyces cerevisiae culture and Saccharomyces cerevisiae live cells on
in vitro mixed ruminal microorganism fermentation. Journal of Dairy Science, 85(10), 2603-2608.
Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D., & Schneider, W. (1979). The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. The Journal of Agricultural Science, 93(1), 217-222.
Norman, H.C., Revell, D. K, Mayberry, D. E., Rintoul, A. J. Wilmot, M. G., & Masters, D.G. (2010). Comparison of in vivo organic matter digestion of native Australian shrubs by sheep to in vitro and in sacco predictions. Small Ruminant Research, 91, 69-80.
Patel, K.P., Katole, S.B., Pandya, P.R., Sorathia, K.K., & Patel, S. (2020). Effect of solid-state fermentation biomass supplementation to mixed substrate on digestibility and methane mitigation in vitro. Indian Journal of Animal Nutrition, 37(2), 127-131.
Pearson, R.M., & Smith, J.A.B. (1943). The utilization of urea in the bovine rumen. 1. Methods of analysis of the rumen ingesta and preliminary experiments in vivo. Biochemical Journal, 37(1), 142.
Pradhan, S.K., Deshpande, S.B., Singh, R.R., & Dabas, V.S. (2021). Effect of active dry yeast supplementation on nutrient digestibility, rumen metabolites and performances of Surti goat kids. Indian Journal of Animal Nutrition, 38(1), 23-30.
Puniya, A.K., Salem, A.Z., Kumar, S., Dagar, S.S., Griffith, G.W., Puniya, M., & Kumar, R. (2015). Role of live microbial feed supplements with reference to 33 anaerobic fungi in ruminant productivity: A review. Journal of Integrative Agriculture, 14(3), 550-560.
Salem, A.Z.M., Robinson, P.H., El- Adawy, M.M., & Hassan, A.A. (2007). In vitro fermentation and microbial protein synthesis of some browse tree leaves with or without addition of polyethylene glycol. Animal Feed Science and Technology, 138, 318-330.
Sallam, S.M.A., Allam, A.M., & Najadi, S.A. (2014). Comparison of two products of direct-fed microbial supplementation on the nutrient utilization and ruminal fermentation in sheep. Journal of Agricultural Science, 6(3), 159.
Sheikh, G.G., Masood, D., Ganai, A.M., Muzamil, S., Afzal, Y., & Ahmad, H.A. (2019). Effect of probiotics mix supplementation on haemato-biochemical parameters and bacterial faecal shedding in Corriedale lambs fed paddy straw based complete feed. Indian Journal of Animal Research, 53(8), 1049-1053.
Sherasia, P.L., Pandya, P.R., Parnerkar, S., Murty, S., & Devalia, B.R. (2017). In vitro digestibility and methane production as affected by incorporation of different levels of solid-state fermented biomass in total mixed rations. Indian Journal of Animal Nutrition, 34(4), 420-424.
Snedecor, G.W., & Cochran, W.G. (1994), Statistical Methods. 8th edn., The Iowa State University press, Ames, Iowa, USA.
Soren, N.M., Tripathi, M.K., Bhatt, R.S., & Karim, S.A. (2013). Effect of yeast supplementation on the growth performance of Malpura lambs. Tropical Animal Health and Production, 45, 547-554.
Tedeschi, L.O., Kononoff, P.J., Karges, K., & Gibson, M.L. (2009). Effects of chemical composition variation on the dynamics of ruminal fermentation and biological value of corn milling (co) products. Journal of Dairy Science, 92, 401-413.
Wang, J., Zhao, G., Zhuang, Y., Chai, J., & Zhang, N. (2022). Yeast (Saccharomyces cerevisiae) culture promotes the performance of fattening sheep by enhancing nutrients digestibility and rumen development. Fermentation, 8(12), 719.
Zhong, R.Z., Sun, H.X., Li, G.D., Liu, H.W., & Zhou, D.W. (2014). Effects of inoculation with rumen fluid on nutrient digestibility, growth performance and rumen fermentation of early weaned lambs. Livestock Science, 162, 154-158.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
