Ovum-Pick Up and In Vitro Embryo Production Responses in Stimulated and Non-Stimulated Sahiwal Cows
DOI:
https://doi.org/10.48165/ijvsbt.20.6.03Keywords:
Embryo, FSH, OPU-IVF, Quality, Sahiwal cowsAbstract
The aim of the present study was to evaluate follicular population, oocyte recovery, quality, and in vitro embryo developmental competence in non-stimulated and FSH stimulated Sahiwal cows subjected to ovum pick-up (OPU) and in vitro embryo production (IVEP). The study was conducted on total 16 cows divided into two groups; group 1 FSH stimulated (n=08) and group 2 non-stimulated (n=08) Sahiwal cows. A total of 16 OPU sessions were performed in early luteal phase of estrous cycles. The mean number of follicles aspirated (17.13±1.87 Vs. 12.38±0.85) and the mean number of oocytes recovered (10.25±0.98 Vs. 6.75±0.60) per OPU per animal were significantly (p<0.05) higher in FSH-stimulated as compared to the non-stimulated cows. In stimulated group, a significantly (p<0.05) high Grade-A oocytes were recovered as compared to the non-stimulated group (60.98±0.49% Vs. 24.07±0.40%). However, maturation rate (84.41 Vs. 81.10%) and fertilization rate (78.46 Vs. 72.08) did not differ significantly between stimulated and non-stimulated groups. Further when fertilized oocytes were cultured in vitro for 7 days higher (p<0.05) cleavage rate (73.44%Vs. 61.25%) and embryo production (4.50±0.50 Vs. 2.38±0.43 per OPU session) were recorded in stimulated cows as compared to the non-stimulated cows. Further all the zygotes cleaved into 2-cell stage in both the groups, but subsequently significantly (p<0.05) more 4-cell stage, 8-cell stage and numerically higher 16-cell stage and compact morula (13.89% Vs. 5.26%) were recorded in stimulated than in non-stimulated Sahiwal cows. In conclusion, the FSH pre-stimulation prior to OPU-IVF increased the number of medium and large follicles, oocyte recovery and embryo quality in Sahiwal cows.
Downloads
References
Aller, J.F., Mucci, N.C., Kaiser, G.G., Rios, G., Callejas, S.S., & Alberio, R.H. (2010). Transvaginal follicular aspiration and embryo development in superstimulated early postpartum beef cows and subsequent fertility after artificial insemination. Animal Reproduction Science, 119(1-2), 1-8.
Blondin, P., Bousquet, D., Twagiramungu, H., Barnes, F., & Sirard, M. A. (2002). Manipulation of follicular development to produce developmentally competent bovine oocytes. Biology of Reproduction, 66(1), 38-43.
Bo, G.A., & Mapletoft, R.J. (2020). Superstimulation of ovarian follicles in cattle: Gonadotropin treatment protocols and FSH profiles. Theriogenology, 150, 353-359.
Chasombat, J., Nagai, T., Parnpai, R., & Vongpralub, T. (2013). Ovarian follicular dynamics, ovarian follicular growth, oocyte yield, in vitro embryo production and repeated oocyte pick up in Thai native heifers undergoing superstimulation. Asian-Australasian Journal of Animal Sciences, 26(4), 488.
Ciftci, M.F., & Dinc, D.A. (2023). The effect of different FSH administration before ovum pick up on superstimulation response and oocyte yield. Reproduction in Domestic Animals, 58(8), 1055-1062.
De Carvalho, J.G.S., de Carvalho, N.A.T., Bayeux, B.M., Watanabe, Y.F., Watanabe, O.Y., Mingoti, R.D., & Baruselli, P.S. (2019). Superstimulation prior to the ovum pick-up improves the in vitro embryo production in nulliparous, primiparous and multiparous buffalo (Bubalus bubalis) donors. Theriogenology, 138, 164-168.
De Roover, R., Feugang, J.M.N., Bols, P.E.J., Genicot, G., & Hanzen, C.H. (2008). Effects of ovum pick-up frequency and FSH stimulation: a retrospective study on seven years of beef cattle in vitro embryo production. Reproduction in Domestic Animals, 43(2), 239-245.
Gimenes, L.U., Ferraz, M.L., Fantinato-Neto, P., Chiaratti, M.R., Mesquita, L.G., Sá Filho, M.F., & Baruselli, P.S. (2015). The interval between the emergence of pharmacologically synchronized ovarian follicular waves and ovum pickup does not significantly
affect in vitro embryo production in Bos indicus, Bos taurus, and Bubalus bubalis. Theriogenology, 83(3), 385-393.
Nawaz, M., Saleem, M., Yaseen, M., Sagheer, M., Zahoor, I., Ahmad, N., & Riaz, A. (2022). Ovarian stimulation through FSH improves follicular harvesting and blastocyst yield in Bos indicus cattle. Pakistan Veterinary Journal, 42(1), 17-24.
Ongaratto, F.L., Rodriguez-Villamil, P., Tribulo, A., & Bó, G.A. (2015). Effect of follicle wave synchronization and gonadotropin treatments on the number and quality of cumulus-oocyte complex obtained by ultrasound-guided ovum pick-up in beef cattle. Animal Reproduction, 12(4), 876-883.
Patil, S.P., Hadiya, K.K., Layek, S.S., Gorani, S., Raj, S., Karuppanasamy, K., & Gupta, R.O. (2022). Ovum pick-up and in vitro embryo production from stimulated vs non-stimulated buffaloes. The Indian Journal of Veterinary Sciences and Biotechnology, 18(1), 96-.
Purwantara, B., Schmidt, M., Greve, T., & Callesen, H. (1993). Follicular dynamics prior to and during superovulation in heifers. Theriogenology, 40(5), 913-921.
Sakaguchi, K., Maylem, E.R.S., Tilwani, R.C., Yanagawa, Y., Katagiri, S., Atabay, E.C., & Nagano, M. (2019). Effects of follicle stimulating hormone followed by gonadotropin releasing hormone on embryo production by ovum pick up and in vitro fertilization in the river buffalo (Bubalus bubalis). Animal Science Journal, 90(5), 690-695.
Sarwar, Z., Saad, M., Saleem, M., Husnain, A., Riaz, A., & Ahmad, N. (2020). Effect of follicle size on oocytes recovery rate, quality, and in-vitro developmental competence in Bos indicus cows. Animal Reproduction, 17, e20200011.
Sathanawongs, A., Rojanasthien, S., Oranratnachai, A., & Sumretprasong, J. (2004). Effect of FSH treatment on number of bovine oocytes retrieved by non-ultrasound guided transvaginal ovum pick-up and their developmental competence. Reproduction, Fertility and Development, 17(2), 277-277.
Sendag, S., Cetin, Y., Alan, M., Hadeler, K.G., & Niemann, H. (2008). Effects of eCG and FSH on ovarian response, recovery rate and number and quality of oocytes obtained by ovum pick-up in Holstein cows. Animal Reproduction Science, 106(1-2), 208-214.
Silvestri, G., Rathje, C.C., Harvey, S.C., Gould, R.L., Walling, G.A., Ellis, P.J., & Griffin, D.K. (2021). Identification of optimal assisted aspiration conditions of oocytes for use in porcine in vitro maturation: A re-evaluation of the relationship between the cumulus oocyte complex and oocyte quality. Veterinary Medicine and Science, 7(2), 465-473.
Vennapureddy, S., Arunakumari, G., Reddy, K.C., Reddy, K.R., & Ambica, G. (2024). Effect of FSH stimulation of ovaries on in vitro maturation of Sahiwal oocytes collected by ovum pick-up (OPU) method. Indian Journal of Animal Research, 58(3), 395-400.
Viana, J. (2018). Statistics of embryo production and transfer in domestic farm animals. Embryo Technology Newsletter, 36(4), 17. Vieira, L.M., Rodrigues, C.A., Netto, A.C., Guerreiro, B.M., Silveira, C.R.A., Freitas, B.G., & Baruselli, P.S. (2016). Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle. Theriogenology, 85(5), 877-886.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
