Isolation and Molecular Characterization of Pasteurella multocida from Ducks
DOI:
https://doi.org/10.48165/ijvsbt.20.5.08Keywords:
Bipolar, Duck cholera, Hya D gene, Pasteurella multocida, Polymerase chain reactionAbstract
Duck pasteurellosis also called as Duck cholera is a highly contagious disease of ducks caused by Pasteurella multocida. It has been found to be most commonly occurring bacterial disease among semi-mature and mature birds with 5 to 100 % morbidity and 0.5 to 80 % mortality rates. The present study was conducted to investigate the suspected outbreak of pasteurellosis in a duck farm. The samples were collected from morbid and dead birds after conducting post-mortem examinations and processed for further isolation, identification and molecular characterization. The samples were found to be positive for Pasteurella multocida by cultural and biochemical tests and confirmed as Pasteurella multocida by using Pasteurella multocida species specific KMT1 primers and capsular type A specific primers. It was further processed for virulence-associated ompH gene. The purified Pasteurella multocida species specific KMT1 product was sequenced and based on nucleotides homology and phylogenetic analysis, the sample was having 99.76 % homology to Pasteurella multocida strain VP161 chromosome. Based on nucleotides homology and phylogenetic analysis of the capsular type-specific hyaD hyaC gene partial nucleotide sequences, the sample was having 98.60% homology to Pasteurella multocida strain PMISC1 HyaD (hyaD) gene specific for capsular type A.
Downloads
References
Abbas, A.M., Abd El-Moaty, D.A.M., Zaki, E.S.A., El-Sergany, E.F., El-Sebay, N.A., Fadl, H.A., & Samy, A.A. (2018). Use of molecular biology tools for rapid identification and characterization of Pasteurella spp., Veterinary World, 11(7), 1006-1014.
Abd-Elsadek, E.L. Hussein, A.E., & Abouelkhair, A.A. (2021). Molecular studies on Pasteurella multocida in ducks. Journal of Current Veterinary Research, 3(1), 1-9.
Akhtar, M. (2013). Isolation, identification and characterization of Pasteurella multocida from chicken and development of oil based vaccine. M.S. Thesis. Bangladesh Agricultural University, Mymensingh, Bangladesh.
Borah, B., Hazarika, R., Deka, P., Saikia, D.P., Doley, M.K., Sinha, S., & Sharma, R.K. (2023). Characterization of Pasteurella multocida and Riemerella anatipestifer of ducks in Assam, India. Indian Journal of Animal Research. doi: 10.18805/IJAR.B-4977
Deka, P., Bhattacharyya, D.K., Sharma, R.K., Saikia, G.K., Pathak, N., Barkakati, B., Neher, S., & Gogoi, A. (2017). Capsular typing and virulence gene profiling of Pasteurella multocida isolated from chickens. International Journal of Chemical Studies, 5(5), 1928-1933.
Eldin, W.F.S., & Reda, L.M. (2016). Epidemiological prevalence of Pasteurella multocida in ducks. Japanese Journal of Veterinary Research, 64 (supplement 2), S251-S255.
Glisson, J.R., Hofacre, C.L. & Christensen, J.P. (2003). Fowl cholera. In: Y.M. Saif, H.J. Barnes, J.R. Glisson, A.M. Fadly, L.R. McDougald and D.E. Swayne. Diseases of Poultry, 11th edn., Ames, Iowa State University Press. USA, pp. 658-675.
Gokben Ozbey, & Adile Muz (2006). Isolation of aerobic bacteria from lungs of chickens showing respiratory disorders and confirmation of Pasteurella multocida by polymerase chain reaction. Veterinary Archives, 76(3), 217-225.
Ievy, S., Khan, M.R.F., Islam, M.A., & Rahman, M.B. (2013). Isolation and identification of Pasteurella multocida from chicken for the preparation of oil adjuvanted vaccine. Bangladesh Journal of Veterinary Medicine, 2, 1-4.
Jonas, M., Morishita, T.Y., Angrick, E.J., & Jahja,J. (2001). Characterization of nine Pasteurella multocida isolates from avian cholera outbreaks in Indonesia. Avian Diseases, 45, 34–42.
Kapoor, V., Katoch, R.C., Sharma, M., & Asrani, R.K. (2004). Pathogenicity test of Pasteurella multocida in mice A:1. Indian Journal of Animal Sciences, 74, 495-496.
Lee, J., Kim, Y.B., & Kwon, M. (2007). Outer membrane protein H for protective immunity against Pasteurella multocida. Journal of Microbiology, 45(2), 179-184.
Luo, Y., Glisson, J.R., Jackwood, M.W., Hancock, R.E., Bains, M., Cheng, I.N., & Wang, C. (1997). Cloning and characterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73. Journal of Bacteriology, 179, 7856-7864.
Markey, B., Leonard, F., Archambault, M., Cullinane, A., & Maguire, D. (2013): Clinical Veterinary Microbiology, 2nd ed., Chapter 21, Mosby Elsevier, p. 307-316.
Mohan, K., & Pradeep Kumar, G. (2008): Pasturellosis in a duck. Veterinary World, 1(12), 367.
OIE (2021). World organization for Animal Health, Terrestrial Manual (2021).
Panna, S.N., Nazmul Hussain Nazir, K.H.M., Rahman, M.B., Ahamed, S., Saroare, M. G., Chakma, S., Kamal, T., & Majumder, U.H. (2015). Isolation and molecular detection of Pasteurella multocida Type A from naturally infected chickens and their histopathological evaluation in artificially infected chickens in Bangladesh. Journal of Advanced Veterinary and Animal Research, 2(3), 338-345.
Ponnusamy, P., Lurthu Reetha,T., Ronald, B. S.M., Puvarajan, B., & Manickam. R. (2018). Molecular detection and characterization of Pasteurella multocida from migratory ducks in the Cauvery delta region of Tamil Nadu. Journal of Entomology and Zoology Studies, 6(2), 2273-2276.
Priyadarshini, A., Kumar, S., Gupta, S.K., Viswas, K.N., Agarwal, R.K., & Singh, V.P. (2014). Cloning and sequence analysis of hsf, an outer membrane protein gene of Pasteurella multocida serotype B: 2. Veterinary World, 7(12), 1090-1093.
Rimler, R.B., & Glisson J.R. (1997). Fowl cholera. In: Diseases of Poultry, 10th edn., Calnek B.W., Barnes H.J., Beard C.W., McDougald L.R. & Saif Y.M., eds. Iowa State University Press, Ames, Iowa, USA, p. 143-159.
Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74 (12), 5463-5467.
Selim-Amany, O., Alrafie-Amira, S., Sabry, E.O., Elsayed-Hemat, S. (2017). Molecular studies on Pasteurella species isolated from ducks. Assiut Veterinary Medical Journal, 63(155), 136-143.
Shivachandra, S.B., Kumar, A.A., Gautam, R., Joseph, S., Saxena, M.K., Chaudhuri, P., & Srivastava, S.K. (2006). Identification of avian strains of Pasteurella multocida in India by conventional and PCR assays. Veterinary Journal, 172, 561-564.
Tirumurugaan, K.G., Chandran, N.D.J., Prabhakar, T.G., Ravikumar, G., Blackall, P.J., & Rajavela, G. (2004). The immunogenicity and pathogenicity of Pasteurella multocida isolated from poultry in Namakkal. Indian Veterinary Journal, 81, 369-371.
Zhangcheng, L., Fangjun, C., Shimei, L., Zuoyong, Z., Yifei, H., Sishi, C., Mengna, J. & Yingying, S. (2018). Molecular characteristics of Pasteurella multocida strains isolated from poultry in China and genetic analysis of strains in terms of the tonb gene. Kafkas Universitesi Veteriner Fakultesi Dergisi, 24(1), 91-98.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
