Cytokine Profiling Associated with Bovine Tuberculosis
DOI:
https://doi.org/10.48165/ijvsbt.20.6.21Keywords:
Bovine tuberculosis, Cytokines, Enzyme-linked immunosorbent assay, Polymerase chainAbstract
Cytokine expression in bovine tuberculous tissues was estimated and compared to that of non-infected healthy tissue samples using ELISA. Twenty tissue samples suspected of bovine tuberculosis were collected and subjected to a preliminary acid-fast staining technique followed by molecular detection using IS6110 PCR. The expression of anti-inflammatory cytokines interleukin IL-4 and IL-13, immunomodulator IL-10, and the pro-inflammatory cytokines tumour necrosis factor (TNF-α) and interferon-gamma (IFN-γ) were estimated in the tissue samples positive for bovine tuberculosis. The dominance of pro-inflammatory cytokines (IFN-γ and TNF-α) over the anti-inflammatory cytokines (IL-4 and IL-13) in the tuberculosis-infected tissues indicated that the pro-inflammatory cytokines played a crucial role in the tissue damage occurring in the disease. Immunomodulatory cytokine IL-10 levels were also significantly increased indicating its role in the maintenance of the balance of the cytokines. Thus, the present study revealed that cytokines play a major role in the immunopathogenesis of bTB.
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Abdalla AE, Lambert N, DuanX, Xie J(2016)Interleukin-10 family and tuberculosis: An old story renewed. Int. J. Biol. Sci. 12(6): 710.
Abramson SL, Gallin JI(1990) IL-4 inhibits superoxide production by human mononuclear phagocytes. J. Immunol. Res144(2): 625-30.
Balcewicz-Sablinska MK, Keane J, Kornfeld H, Remold HG(1998) Pathogenic Mycobacterium tuberculosis evades apoptosis of host macrophages by release of TNF-R2, resulting in inactivation of TNF-α. J. Immunol 161(5): 2636-41.
Bilenki L, Gao X, Wang S, Yang J, Fan Y, Han X, Qiu H, Yang X (2010) Dendritic cells from mycobacteria-infected mice inhibits established allergic airway inflammatory responses to ragweed via IL-10- and IL-12-secreting mechanisms. J Immunol. 184(12):7288-96.
Coetsier C, Vannuffel P, Blondeel N, Denef JF, Cocito C, Gala JL (2000) Duplex PCR for differential identification of Mycobacterium bovis, M. avium, and M. avium subsp. Paratuberculosis in formalin- fixed paraffin-embedded tissues from cattle. J Clin Microbiol. 38(8):3048-3054
Dhanasekaran S, Jenum S, Stavrum R, Ritz C, Faurholt-Jepsen D, Kenneth J, Vaz M, Grewal HM, Doherty TM (2013) TB Trials Study Group. Identification of biomarkers for Mycobacterium tuberculosis infection and disease in BCG-vaccinated young children in Southern India. Genes Immun. 14(6):356-64.
de Souza Figueiredo EE, Silvestre FG, Campos WN, Furlanetto LV, Medeiros L, Lilenbaum W, Fonseca LS, Silva, JT, Paschoalin, V M (2009). Identification of Mycobacterium bovis Isolates by a multiplex PCR.Braz J Microbiol. 40(2), 231–233.
Filia G, Leishangthem GD, Mahajan V, Singh A (2016) Detection of Mycobacterium tuberculosis and Mycobacterium bovis in Sahiwal cattle from an organized farm using ante-mortem techniques. Vet. World 9(4): 383.
Flynn J L, Chan J. (2001) Immunology of tuberculosis. Annu. Rev. Immunol. 19(1): 93-129.
García-Bocanegra I, Barranco I, Rodríguez-Gómez IM, Pérez B, Gómez-Laguna J, Rodríguez S, Ruiz-Villamayor E, Perea A (2010) Tuberculosis in alpacas (Lama pacos) caused by Mycobacterium bovis. J Clin Microbiol. 48(5):1960-4.
Gupta A, Leishangthem GD, Gupta K, Sood NK, Singh A(2016) Cytokine Expression studies in natural cases of bovine lymphadenopathies. Asian J Anim Vet Adv 1(9): 556-62.
Harris J, De Haro SA, Master SS, Keane J, Roberts EA, Delgado M, Deretic V (2007) T helper 2 cytokines inhibit autophagic control of intracellular Mycobacterium tuberculosis. Immunity 27(3):505-17
Howard AD,Zwilling BS(1999) Reactivation of tuberculosis is associated with a shift from type 1 to type 2 cytokines. Clin. Exp. Immunol.115(3): 428.
Jamil B, Shahid F, Hasan Z, Nasir N, Razzaki T, Dawood G, Hussain R (2007) Interferon gamma/IL10 ratio defines the disease severity in pulmonary and extra pulmonary tuberculosis. Tuberculosis 87(4):279-87.
Kindler V, Sappino AP, Grau GE, Piguet PF, Vassalli P(1989) The inducing role of tumor necrosis factor in the development of bactericidal granulomas during BCG infection. Cell 56(5): 731-40.
Liébana E, Aranaz A, Mateos A, Vilafranca M, Gomez-Mampaso E, Tercero JC, Alemany J, Suarez G, Domingo M, Dominguez L (1995) Simple and rapid detection of Mycobacterium tuberculosis complex organisms in bovine tissue samples by PCR. J Clin Microbiol. 33(1):33-6.
Palmer MV, Thacker TC, Waters WR (2016) Differential cytokine gene expression in granulomas from lungs and lymph nodes of cattle experimentally infected with aerosolized Mycobacterium bovis. PloSone 11(11): e0167471.
Ramachandra L, Simmons D, Harding CV(2009) MHC molecules and microbial antigen processing in phagosomes. Curr. Opin. Immunol. 21(1): 98-104.
Rhodes SG, Buddle BM, Hewinson RG, Vordermeier HM (2000) Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease. Immunology 99(2): 195-202.
Rook WAG (2007) Th2 Cytokines in susceptibility to tuberculosis. Curr. Mol. Med.7(3). https://dx.doi.org/10.2174/156652407780598557
Saunders BM and Britton WJ (2007) Life and death in the granuloma: immunopathology of tuberculosis. Immunol. Cell Biol. 85(2): 103-11.
Sharma DK, Singh ND, Leishangthem GD and Banga HS (2020) Amelioration of bleomycin-induced acute lung injury and epithelial-mesenchymal transition by baicalein in mice. Curr. Sci..119(6):962-972
Sugawara I, Yamada H, Mizuno S, Iwakura Y (2000) IL-4 Is Required for defence against Mycobacterial Infection. Microbiol. Immunol. 44(12): 971-79.
Thacker TC, Harris B, Palmer MV, Waters WR (2011) Improved specificity for detection of Mycobacterium bovis in fresh tissues using IS 6110 real-time PCR. BMC Vet. Res. 7(1), 50.
Wang Y, Zhou X, Lin J, Yin F, Xu L, Huang Y, Ding T, Zhao D (2011) Effects of Mycobacterium bovis on monocyte-derived macrophages from bovine tuberculosis infection and healthy cattle. FEMS Microbiol Lett. 321(1):30-6.
Widdison S, Schreuder LJ, Villarreal-Ramos B, Howard CJ, Watson M, Coffey TJ (2006) Cytokine expression profiles of bovine lymph nodes: effects of Mycobacterium bovis infection and bacilleCalmette-Guérin vaccination. Clin Exp Immunol. 144(2):281-9.
Witchell J, Maddipatla SVPK, Wangoo A, VordermeierM, Goyal M (2010) Time dependent expression of cytokines in Mycobacterium bovis infected cattle lymph nodes. Vet Immunol Immunopathol.138(1-2): 79-84.
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