Oocyte and Embryo Cryopreservation in Porcine In Vitro Production Systems

Authors

  • Hiep Thi Nguyen 1Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Tamas Somfai Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan
  • Bui Xuan Nguyen 3Southest Asia Biotechnology Center, Hanoi, Vietnam
  • Kazuhiro Kikuchi Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan
  • Nguyen Thi Uoc Southest Asia Biotechnology Center, Hanoi, Vietnam
  • Nguyen Viet Linh Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Nguyen Thi Nhung Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Nguyen Thi Hong Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Nguyen Van Hanh Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

DOI:

https://doi.org/10.48165/ijvsbt.20.4.01

Keywords:

Cryopreservation,, Embryos,, In vitro,, Porcine oocytes,, Vitrification.

Abstract

Cryopreservation of oocytes and embryos, in addition to sperm, are important for gene banking and flexible use of time and space. In pigs,  in vitro production (IVP) is not only important system for the utilization of sperm kept in gene banks, but also is one of basic technologies  for the production of genetically modified, disease models or xeno-transplantation materials for human. Porcine oocytes and embryos  are characterized by high lipid content that are very sensitive with cooling. Besides, in vitro embryos are weaker than those derived in vivo in term of cell number. Therefore, cryopreservation by vitrification is preferred to slow freezing. Especially, cryopreservation of porcine  oocytes is possible only by vitrification and germinal vesicle (GV) stage is better than MII stage in terms of cytoskeletal organization  and subsequent blastocyst formation. There are several different vitrification methods that have been applied on porcine IVP embryos  based on the difference in cryoprotectants or devices to date. In this review, we discuss about recent IVP system, cryopreservation of  oocytes and embryos in pigs.

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Published

2024-07-01

Issue

Vol. 20 No. 4 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Review Article

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Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

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How to Cite

Nguyen, H.T., Somfai, T., Nguyen, B.X., Kikuchi, K., Uoc, N.T., Linh, N.V., … Hanh, N.V. (2024). Oocyte and Embryo Cryopreservation in Porcine In Vitro Production Systems . Indian Journal of Veterinary Sciences and Biotechnology, 20(4), 1–5. https://doi.org/10.48165/ijvsbt.20.4.01
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