Screening of SNPs Associated with Egg Production in Two Strains of White Leghorn

Authors

  • Ankit V Kachchhi Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat-388001, India
  • Ashish C Patel Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat-388001, India
  • Glory S Parmar Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat-388001, India
  • Devanshi V Patel Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat-388001, India
  • Prakash G Koringa Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat-388001, India
  • F P Savaliya Poultry Research Station, Kamdhenu University, Anand, Gujarat-388001, India

DOI:

https://doi.org/10.48165/ijvsbt.20.3.31

Keywords:

Egg production, SNPs, White Leghorn

Abstract

The poultry industry in India has become a prominent and rapidly expanding sector of the agro-animal industry, and is recognized as an  important source of animal protein in the country. Through genetic analysis, Single Nucleotide Polymorphisms (SNPs) can help identify  variations in DNA sequences that are linked to traits such as egg quality, quantity, fertility, hatchability, and other important aspects  of egg production in poultry or other egg-laying species. The aim of the present study was to screen the SNPs in the candidate genes  associated with egg production, viz., MC4R, MTNR1A, DRD1, PRLR and VLDLR and their association with egg production at the age of 64  weeks in two strains of White Leghorn, viz., Anand Bantamized White Leghorn (ABWLH) and Anand Synthetic White Leghorn (ASWLH).  The amplicon-sequenced data of a total of 96 birds for these genes were collected in ‘fastq’ format and various SNPs were identified. A  total of 143 detected SNPs were filtered based on read depth and QUAL score and 60 SNPs were discarded after filtering variants. Out  of the remaining 83 SNPs, 69 SNPs were previously reported, while 14 were found as novel. Association analysis showed that one of the  SNPs found in the MC4R gene was significantly associated with the egg production in both ABWLH and ASWLH population. 

Downloads

Download data is not yet available.

References

Al-Hassani, D.H., Razuki, W.M., & Abdulwahid, H.S. (2023). Variations and expression of very low-density lipoprotein receptor (VLDLR) mRNA in the liver and ovary of a low egg production strain of local Iraqi hens. Iraqi Journal of Agricultural Sciences, 54(1), 114-123.

AL-Jaryan, I.L.H., Hassan, W.S., & AL-Rekabi, M.M. (2021). Melatonin receptor A gene, melatonin receptor C gene and ovocalyxin-32 gene polymorphisms associated with some egg production in Local Iraqi chicken. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3631-3639.

Alsiddig, M.A., Yu, S.G., Pan, Z.X., Widaa, H., Badri, T.M., Chen, J., & Liu, H.L. (2017). Association of single nucleotide polymorphism in melatonin receptor 1A gene with egg production traits in Yangzhou geese. Animal Genetics, 48(2), 245-249.

Burt, D.W. (2005). Chicken genome: current status and future opportunities. Genome Research, 15(12), 1692-1698. Demir, E., Karsli, T., Fidan, H., Karsli, B., Aslan, M., Aktan, S., & Balcioğlu, M. (2020). Polymorphisms in some candidate genes associated with egg yield and quality in five different white layer pure lines. Kafkas Universitesi Veteriner Fakultesi Dergisi, 26(6), 735-741.

Feng, P., Zhao, W., Xie, Q., Zeng, T., Lu, L., & Yang, L. (2018). Polymorphisms of melatonin receptor genes and their associations with egg production traits in Shaoxing duck. Asian-Australasian Journal of Animal Sciences, 31(10), 1535-1541.

Gholami, M., Ghaffari, M., & Hashemi, A. (2020). Study of dopamine receptor gene polymorphisms and their association with growth and egg production traits in West Azerbaijan native chicken. Iranian Journal of Applied Animal Science, 10(1), 149-154.

Guo, C., McDowell, I.C., Nodzenski, M., Scholtens, D.M., Allen, A.S., Lowe, W.L., & Reddy, T.E. (2017). Transversions have larger regulatory effects than transitions. BMC Genomics, 18, 1-9.

Guo, S., Bai, Y., Zhang, Q., Zhang, H., Fan, Y., Han, H., & Liu, Y. (2021). Associations of CALM1 and DRD1 polymorphisms, and their expression levels, with Taihang chicken egg-production traits. Animal Biotechnology, 34(4), 1-11.

Hansen, K.A., Zhang, Y., Colver, R., Tho, S.P., Plouffe Jr, L., & McDonough, P.G. (2005). The dopamine receptor D2 genotype is associated with hyperprolactinemia. Fertility and Sterility, 84(3), 711-718.

Hong, K.Y., Wang, F., Yu, D.B., & Du W,X. (2007). Polymorphisms in Taihu chicken of PRL, PRLR and FSH-β genes and association with prophase egg production. Acta Agric Borealiocci dent Sinica, 16(5), 11-14.

Karim, E.S., & Aggag, S. (2018). Association of single nucleotide polymorphism in melanocortin receptor gene with egg production traits in Lohmann Brown chickens. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 24(1), 145-148.

Karsli, T., Demir, E., Fidan, H.G., Karsli, B.A., Aslan, M., Aktan, S., & Balcioğlu, M. (2020). Polymorphisms in candidate genes associated with egg yield and quality in brown layer pure lines. Mediterranean Agricultural Sciences, 33(3), 433-439.

Kelly, P.A., Binart, N., Lucas, B., Bouchard, B., & Goffin, V. (2001). Implications of multiple phenotypes observed in prolactin receptor knockout mice. Frontiers in Neuroendocrinology, 22(2), 140-145.

Ko, E., Seo, H.W., Jung, E.S., Kim, B.H., & Jung, G. (2016). The TERT promoter SNP rs2853669 decreases E2F1 transcription factor binding and increases mortality and recurrence risks in liver cancer. Oncotarget, 7(1), 684-699.

Kulibaba, R.A. (2015). Polymorphism of growth hormone, growth hormone receptor, prolactin and prolactin receptor genes in connection with egg production in Poltava clay chicken. Journal of Agricultural Sciences, 2(5), 198-207.

Li, D.Y., Wu, N., Tu, J.B., Hu, Y.D., Yang, M.Y., Yin, H.D., & Zhu, Q. (2015). Expression patterns of melatonin receptors in chicken ovarian follicles affected by monochromatic light. Genetics and Molecular Research, 14(3), 10072-10080.

Liu, G., Zeng, M., Li, X., Rong, Y., Hu, Z., Zhang, H., & Liu, X. (2021). Expression and analysis of ESR1, IGF-1, FSH, VLDLR, LRP, LH, PRLR genes in Pekin duck and Black Muscovy duck. Gene, 76(9), 145-183.

Luo, P.T., Yang, R.Q., & Yang, N. (2007). Estimation of genetic parameters for cumulative egg numbers in a broiler dam line by using a random regression model. Poultry Science, 86(1), 30-36.

MacNeil, D.J., Howard, A.D., Guan, X., Fong, T.M., Nargund, R.P., Bednarek, M.A., ... & Van der Ploeg, L.H. (2002). The role of melanocortins in body weight regulation: opportunities for the treatment of obesity. European Journal of Pharmacology, 450(1), 93-109.

Meng, X.T., Hou, N.N., Wang, X.J., Jiao, H.C., Zhao, J.P., Song, Z.G., & Lin, H. (2013). Increased hepatic yolk precursor synthesis,

secretion and facilitated uptake by follicles are involved in the rejuvenation of reproductive performance of molted hens (Gallus gallus domesticus). General and Comparative Endocrinology, 194, 198-207.

Pal, S., Patel, A.C., Hinsu, A.T., Panchal, K.J., Koringa, P.G., & Rank, D.N. (2023). The Identification of single nucleotide polymorphisms in GnRH-I, GnRH-II/MRPS26, GnRHR exons and their association with egg production in Anand Synthetic White Leghorn and Anand Bantamised White Leghorn Chicken. Indian Journal of Veterinary Sciences & Biotechnology, 19(2), 94-98.

Qin, N., Liu, Q., Zhang, Y.Y., Fan, X.C., Xu, X.X., Lv, Z.C., & Xu, R.F. (2015). Association of novel polymorphisms of fork head box L2 and growth differentiation factor-9 genes with egg production traits in local Chinese Dagu hens. Poultry Science, 94(1), 88-95.

Sundaresan, N.R., Leo, M., Subramani, J., Anish, D., Sudhagar, M., Ahmed, K.A., & Saxena, V.K. (2009). Expression analysis of melatonin receptor subtypes in the ovary of domestic chicken. Veterinary Research Communications, 33(1), 49-56.

Vinh, N.T., Giang, N.T.P., Linh, N.V., Dang, P.K., Cahn, N.X., Giang, N.T.C., & Thinh, N.H. (2021). Single nucleotide polymorphisms of candidate genes related to egg production traits in Vietnamese indigenous chickens. Brazilian Journal of Poultry Science, 23(2), 1-6.

Weng, Z., Xu, Y., Li, W., Chen, J., Zhong, M., Zhong, F., Du, B., Zhang, B., & Huang, X. (2020). Genomic variations and signatures of selection in Wuhua yellow chicken. Plos One, 15(10), e0241137.

Yan, L.J., Fang, X.T., Liu, Y., Zhang, C.L., Liu, X.X., Zhao, J., & Chen, H. (2013). Effects of single and combined genotypes of MC4R and POU1F1 genes on two production traits in Langshan chicken. Molecular Biology Reports, 40(7), 4645-4650.

Zhou, Y., Han, H.X., Lei, Q.X., Gao, J.B., Liu, W., Li, F.W., & Cao, D.G. (2020). Associations of diplotypes of VLDLR gene with egg production traits in Laiwu Black chickens. Pakistan Journal of Zoology, 52(6), 01-06.

Downloads

Published

2024-05-10

Issue

Vol. 20 No. 3 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Research Article

License

Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kachchhi, A.V., Patel, A.C., Parmar, G.S., Patel, D.V., Koringa, P.G., & Savaliya, F.P. (2024). Screening of SNPs Associated with Egg Production in Two Strains of White Leghorn . Indian Journal of Veterinary Sciences and Biotechnology, 20(3), 158–162. https://doi.org/10.48165/ijvsbt.20.3.31
Crossref
Scopus
Google Scholar
Europe PMC