Physico-Morphological Attributes, Freezability, Fertility and their Correlations in Jaffarabadi Buffalo Bull Semen

Authors

  • Hardipsinh P Vijyeta Cattle Breeding Farm, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Fulabhai S Kavani Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Arshi A Vagh Department of Veterinary Medicine, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Karsan B Vala Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Harish H Savsani Department of Animal Nutrition, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Pratik S Kapadiya Livestock Research Station, Kamdhenu University, Sardarkrushi Nagar-385506, Gujarat, India
  • Rupesh J Raval Polytechnic in Animal Husbandry, College of Veterinary Science & AH, Kamdhenu University, S.K. Nagar-385506, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.20.3.02

Keywords:

Additive sericin, Cryopreservability, Fertility, Jaffarabadi buffalo semen, Pearson’s correlations

Abstract

The present study was carried out on semen of six Jaffarabadi buffalo bulls, aged 6-8 years, under twice weekly semen collection  schedule. In all 36 semen ejaculates (6/bull) were evaluated for routine physical characteristics including sperm quality and oxidative  stress parameters at pre-freeze and post-thaw stages of cryopreservation and fertility using different Sericin concentrations in AndroMed® extender. All bulls donated milky-white semen with identical mean mass motility score, while ejaculate volume and sperm concentration  differed significantly among bulls. The values of sperm progressive motility (%), viability (%), abnormality (%), HOST reactivity (%),  acrosome integrity (%), lipid peroxidation (MDA µM) and total antioxidant capacity (TAC µM) of pre-freeze (on dilution) and post-thawed  semen averaged 84.24 ±0.24 & 53.42±0.32, 82.43±0.26 & 57.38±0.49, 6.14±0.12 & 13.56±0.22, 72.84±0.31 & 45.65±0.48, 81.56±0.35 &  49.73 ±0.52, 7.71±0.04 & 8.35±0.05, and 315.80±5.99 & 292.89±7.40, respectively. The overall first service conception rate achieved  was 36.73 (551/1500) %, and it varied from 28.00 to 44.66% between levels of Sericin. Pearson’s correlations amongst the pre-freeze as  well as post-thawed semen parameters, and of pre-freeze with post-thawed semen parameters including CRs of frozen-thawed semen  worked out using bull-wise and additive-wise 30 mean values revealed significant positive associations among sperm motility, viability,  acrosome integrity, HOST reactive sperm, total antioxidant capacity (TAC), and first service CR, and all these had negative correlations  with abnormal sperm and the lipid peroxidation (MDA) status in both pre-freeze and post-thawed semen. Further, the post-thawed  sperm quality and fertility could be predicted well with highly significant positive correlations of pre-freeze sperm quality parameters  and TAC in Jaffarabadi buffalo bulls. 

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Published

2024-05-10

Issue

Vol. 20 No. 3 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Research Article

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Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

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How to Cite

Vijyeta, H.P., Kavani, F.S., Vagh, A.A., Vala, K.B., Savsani, H.H., Kapadiya, P.S., & Raval, R.J. (2024). Physico-Morphological Attributes, Freezability, Fertility and their Correlations in Jaffarabadi Buffalo Bull Semen . Indian Journal of Veterinary Sciences and Biotechnology, 20(3), 8–14. https://doi.org/10.48165/ijvsbt.20.3.02
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