Occurrence of G10P[11] Genotype of Group A Bovine Rotavirus in Diarrheic Calves from in and around Navsari, Gujarat

Authors

  • Pushpa M Makwana Department of Veterinary Microbiology, College of Veterinary Science & A.H., Kamdhenu University, Navsari- 396450, Gujarat, India
  • Irsadullahkhan H Kalyani Department of Veterinary Microbiology, College of Veterinary Science & A.H., Kamdhenu University, Navsari- 396450, Gujarat, India
  • Dhruv N Desai Department of Veterinary Microbiology, College of Veterinary Science & A.H., Kamdhenu University, Navsari- 396450, Gujarat, India
  • Priti D Vihol Department of Veterinary Pathology, College of Veterinary Science & A.H., Kamdhenu University, Navsari-396450, Gujarat, India
  • Jignesh M Patel Department of Veterinary Pathology, College of Veterinary Science & A.H., Kamdhenu University, Navsari-396450, Gujarat, India
  • Jayesh B Solanki Dept. of Veterinary Parasitology, College of Veterinary Science & A.H., Kamdhenu University, Navsari-396450, Gujarat, India
  • Dharmesh R Patel Department of Veterinary Microbiology, College of Veterinary Science & A.H., Kamdhenu University, Navsari- 396450, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.20.3.25

Keywords:

Bovine calves, Genotyping, G-P typing, Rotavirus A, RNA-PAGE, RT-PCR

Abstract

A study was undertaken to ascertain genotypic characterization of bovine group A rotavirus circulating in the Navsari region of Gujarat  state. Total 157 diarrheic fecal samples were collected from bovine calves of organized farms and locally at village level from Navsari  region. All the samples were initially screened by RNA-PAGE and RT-PCR followed by characterization by sequencing and G-P typing.  The study revealed 10 (6.36%) samples positive by RNA-PAGE, while 16 (10.19%) samples were positive by VP4, VP6 and VP7 gene based RT-PCR. From these positive samples, representative samples were sequenced for complete coverage of VP4, VP6, and VP7 genes  followed by P and G typing on the basis of VP4 and VP7 sequence results, respectively. All the sequenced samples revealed G10 and  P[11] types, showing a G10P[11] combination significantly prevailing in the bovine calves. Phylogenetic analysis of VP4, VP6, and VP7  genes was performed to determine the cross-species transmission dynamics and regional diversity, which showed similarity with other  isolates circulating in India. Our findings suggested that a zoonotic potential genotype G10P[11] is the most circulating genotypes of  Rotavirus A in bovine calves from Navsari region of Gujarat, India.  

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References

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Published

2024-05-10

Issue

Vol. 20 No. 3 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Research Article

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Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

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How to Cite

Makwana, P.M., Kalyani, I.H., Desai, D.N., Vihol, P.D., Patel, J.M., Solanki, J.B., & Patel , D.R. (2024). Occurrence of G10P[11] Genotype of Group A Bovine Rotavirus in Diarrheic Calves from in and around Navsari, Gujarat . Indian Journal of Veterinary Sciences and Biotechnology, 20(3), 124–131. https://doi.org/10.48165/ijvsbt.20.3.25
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