Effect of Supplementation of Antioxidant Sericin in Semen Extender on Post-Thaw Sperm Quality, Oxidative Status and Fertility of Jaffarabadi Buffalo Bull Semen

Authors

  • Hardipsinh P Vijyeta Assistant Research Scientist, Cattle Breeding Farm, Kamdhenu University, Junagadh-362001, Gujarat, India.
  • Fulabhai S Kavani Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Arshi A Vagh Department of Veterinary Medicine, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Gajendrasingh B Solanki Cattle Breeding Farm, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Karsan B Vala Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Vivek K Singh Department of Veterinary Physiology & Biochemistry, College of Veterinary Science & AH, Kamdhenu University, Junagadh-362001, Gujarat, India
  • Arjan J Dham Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & AH, Kamdhenu University, Anand-388001, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.20.2.16

Keywords:

Cryopreservation, Fertility, Jaffarabadi buffalo semen, Oxidative markers, Sericin

Abstract

The present investigation was undertaken on semen of six Jaffarabadi buffalo bulls, aged 6-8 years (n=6x6=36 ejaculates) with an  objective of determining the influence of different concentrations of Sericin, viz., 0.0% control (T0), 0.25 % (T1), 0.50 % (T2), 0.75 %  (T3), 1.0 % (T4) in AndroMed® extender on the quality of sperm parameters, oxidative status and fertility of cryopreserved semen.  The semen samples were evaluated at pre-freeze (on dilution) and post-thaw stages. The pre-freeze sperm motility, viability, plasma  membrane integrity, acrosome integrity and total antioxidant capacity of semen were significantly (p<0.05) higher with lower sperm  abnormalities in control and 0.25% or 0.50% sericin supplemented extender over others, while in post-thawed semen the values of  all these parameters were significantly (p<0.01) higher with lower sperm abnormalities in 0.25% followed by 0.50% Sericin groups  over control and 0.75% Sericin, while 1.0% was found to be detrimental by suppressing all sperm parameters even below the control  values. Lipid peroxidation (MDA production) status however did not vary much between treatment, although it was lower (p<0.05)  with 0.25% and 0.50% Sericin at post-thaw stage. The first service conception rates of frozen-thawed semen with inclusion of 0.25 %  and 0.50 % Sericin in extender were significantly higher (44.66±1.43 % and 42.66±1.76%) as compared to those of 0 %, 0.75%, and 1%  concentrations (38.66±1.33, 29.66±1.89 and 28.66±2.13 %, respectively). Thus the study concluded that since supplementation of Sericin  @ 0.25 or 0.50% (w/v) concentration in AndroMed® extender significantly improved post-thawed sperm quality parameters, oxidative  status and fertility of cryoprserved Jaffarabadi buffalo semen over control and higher levels, it may be added routinely in the extender  during cryopreservation of buffalo semen for better outcome.  

Downloads

Download data is not yet available.

References

Akhter, S., Ansari, M.S., Rakha, B.A., Andrabi, S.M.H., Anwar, M., & Ullah, N. (2010). Effect of fructose addition in skim milk extender on the quality of liquid Nili-Ravi buffalo (Bubalus bubalis) semen. Pakistan Journal of Zoology, 42(3), 227-231.

Andrabi, S.M.H. (2009). Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reproduction in Domestic Animals, 44(3), 552-569.

Andrabi, S.M.H., Akhter, S., Ansari, M.S., Ullah, N., & Qayyum, M. (2008). Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reproduction in Domestic Animals, 43(3), 272-278.

Anzar, M., Farooq, U., Mirza, M. A., Shahab, M., & Ahmad, N. (2003). Factors affecting the efficiency of artificial insemination in cattle and buffalo in Punjab, Pakistan. Pakistan Veterinary Journal, 23(3), 106-113.

Cao, T.T., & Zhang, Y.Q. (2017). The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation. Amino Acids, 49, 1029-1039.

Chaturvedi, D., Dhami, A.J., Chaudhari, D.V. & Patel, A.C. (2023). Evaluation of cryoprotective, cryocapacitation inhibitory and fertility enhancing potential of RU-486, sericin and taurine in cattle and buffalo semen. Indian Journal of Animal Research, online published, DOI: 10.18805/IJAR.B-4913.

Chaturvedi, D., Dhami, A. J., Chaudhari, D. V., & Pathan, M. M. (2021). Effect of mifepristone, sericin and taurine in tris extender on oxidative markers and quality of fresh and frozen-thawed bovine spermatozoa. International Journal of Livestock Research, 10(12), 61-67.

Crespilho, A.M., Sá Filho, M.F., Dell’Aqua Jr, J.A., Nichi, M., Monteiro, G.A., Avanzi, B.R., Martins, A., & Papa, F.O. (2012). Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin-based extenders. Livestock Science, 149(1-2), 1-6.

Dash, R., Acharya, C., Bindu, P.C., & Kundu, S.C. (2008). Antioxidant potential of silk protein sericin against hydrogen peroxide induced oxidative stress in skin fibroblasts. BMB Reproduction, 41, 236-241.

Demra, R., Gandotra, V.K., Singh, A.K., & Kumar, A. (2017). Validation of Sericin concentration in semen freezing media and its effect on post-thaw quality of buffalo bull semen. Veterinary Practitioner, 18(1), 42-44.

Dhami, A.J., Chaturvedi, D., Thesia, M.J., Chaudhari, D.V., & Patel, B.S. (2020). Role of extender additives and AI technicians in improving first service conception rate with frozen semen in cattle and buffaloes under field conditions. Indian Journal of Animal Reproduction, 41(2), 45-50.

Gordon, I. (1996). Controlled Reproduction in Cattle and Buffaloes. CAB International. Willingford, UK, p. 432-466.

Jeyendran, R.S., Van der Ven, H.H., Perez-Pelaez, M., Crabo, B.G., & Zaneveld, L.J.D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Reproduction, 70(1), 219-228.

Kadirvel, G., Kumar, S., & Kumaresan, A. (2009). Lipid peroxidation, mitochondrial membrane potential and DNA integrity of spermatozoa in relation to intracellular reactive oxygen species in liquid and frozen-thawed buffalo semen. Animal Reproduction Science, 114(1-3), 125-134.

Kato, N., Sato, S., Yamanaka, A., Yamada, H., Fuwa, N., & Nomura, M. (1998). Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Bioscience, Biotechnology and Biochemistry, 62(1), 145-147.

Khan, D.R., Ahmad, N., Anzar, M., & Channa, A.A. (2009). Apoptosis in fresh and cryopreserved buffalo sperm. Theriogenology, 71(5), 872-876.

Kumar, P., Kumar, D., Sikka, P., & Singh, P. (2015). Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Animal Reproduction Science, 152, 26-31.

Kutty, I.C., Joseph, M., & Iyer, C.P.N. (1996). A simplified nigrosine eosin-Geimsa staining technique to distinguish acrosome damaged live and dead spermatozoa. Indian Journal of Animal Research, 17(2), 122-123.

Masakazu, T., Kazuhisa, T., Hideyuki, Y., Hiroshi, T., & Shigeru, N. (2003). The silk protein, sericin, protects against cell death caused by acute serum deprivation in insect cell culture. Biotechnology Letters, 25, 1805-1809.

Patel, T.M., Dhami, A.J., Chaudhari, D.V., & Pathan, M.M. (2019). Influence of antioxidant sericin in tris extender on oxidative markers during cryopreservation (–196ºC) of bovine semen. The Indian Journal of Veterinary Sciences & Biotechnology, 15(2), 34-37.

Sasaki, M., Kato, Y., Yamada, H., & Terada, S. (2005). Development of a novel serum-free freezing medium for mammalian cells using the silk protein sericin. Biotechnology and Applied Biochemistry, 42(2), 183-188.

Snedecor, G.W., & Cochran, W.G. (1994). Statistical Methods. 6th edn., Oxford and IBH Publishing Company, Calcutta, India. Terada, S., Nishimura, T., Sasaki, M., Yamada, H., & Miki, M. (2002). Sericin, a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including hybridoma. Cytotechnology, 40, 3-12.

Downloads

Published

2024-03-10

Issue

Vol. 20 No. 2 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2024

Section

Research Article

License

Copyright (c) 2024 Indian Journal of Veterinary Sciences & Biotechnology

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Vijyeta, H.P., Kavani, F.S., Vagh, A.A., Solanki, G.B., Vala, K.B., Singh, V.K., & Dham, A.J. (2024). Effect of Supplementation of Antioxidant Sericin in Semen Extender on Post-Thaw Sperm Quality, Oxidative Status and Fertility of Jaffarabadi Buffalo Bull Semen . Indian Journal of Veterinary Sciences and Biotechnology, 20(2), 79–84. https://doi.org/10.48165/ijvsbt.20.2.16
Crossref
Scopus
Google Scholar
Europe PMC