Effect of Streptomycin-Penicillin and Gentamicin-Tylosin- Lincomycin-Spectinomycin on the Quality and Bacterial Load of Frozen-Thawed Semen of Surti Buffalo Bull

Authors

  • Devki Nandan Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur-313601, Rajasthan, India
  • Mitesh Gaur Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur-313601, Rajasthan, India
  • Deepak Sharma Department of Microbiology, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur-313601, Rajasthan, India
  • Kalpesh Pargi Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur-313601, Rajasthan, India
  • Vachna Ram Department of Veterinary Gynaecology and Obstetrics, College of Veterinary and Animal Science, Navania, Vallabhnagar, Udaipur-313601, Rajasthan, India

DOI:

https://doi.org/10.48165/ijvsbt.20.2.05

Keywords:

Antibiotic combinations, Bacterial load, Buffalo semen, Cryopreservation, Semen Quality

Abstract

The present study evaluated the effect of two different antibiotic combinations SP (streptomycin, penicillin) and GTLS (gentamicin,  tylosin, lincomycin and spectinomycin), as antibiotic regimens in TCFY (Tris-citric acid-fructose-yolk) semen extender on Surti buffalo  bull semen. A total of 12 ejaculates from 6 bulls were collected. After evaluation of the dilution rate, the fresh semen was divided into  three parts. Each part of the semen was extended upto 80 million spermatozoa/mL. One control group (part 1) was maintained with  no antibiotics, while antibiotic combination SP and GTLS was added into part 2 and part 3 at the concentration of streptomycin 1000  µg/mL, penicillin 1000 IU/mL (Treatment-1), and gentamicin 500 µg/mL, tylosin 100 µg/mL, lincomycin 300 µg/mL and spectinomycin  600 µg/mL (Treatment-2). Pre-freeze and post-thaw microbial load (CFU/mL) and sperm abnormalities were found to be significantly  (p<0.05) lower, whereas individual progressive sperm motility, live sperm percentage and HOS responsive spermatozoa were significantly  (p<0.05) higher in semen samples treated with antibiotic combinations SP and GTLS in comparison to control. Further, the GTLS had  a significantly (p<0.05) lower bacterial load at the pre-freeze and post-thaw stage as compared to SP. However, the observations on  sperm parameters with SP and GTLS were statistically similar. It was concluded that SP and GTLS in semen extender show significant  improvement in pre-freeze and post-thawed semen quality, statistically both at par as compared to control, and more efficiently reduced  the bacterial load compared to SP and non-added control suggesting its preference for use in bovine frozen semen extender.  

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Published

2024-03-10

Issue

Vol. 20 No. 2 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar-Apr) 2024

Section

Research Article

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Copyright (c) 2024 Indian Journal of Veterinary Sciences & Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Nandan, D., Gaur, M., Sharma, D., Pargi, K., & Ram, V. (2024). Effect of Streptomycin-Penicillin and Gentamicin-Tylosin- Lincomycin-Spectinomycin on the Quality and Bacterial Load of Frozen-Thawed Semen of Surti Buffalo Bull. Indian Journal of Veterinary Sciences and Biotechnology, 20(2), 24–27. https://doi.org/10.48165/ijvsbt.20.2.05
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