Effect of Carnitine on Physico-Morphological Properties of Cryopreserved Hariana Bull Spermatozoa

Authors

  • Brijesh Kumar Yadav Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa Vigyan Viswavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Kavisha Gangwar epartment of Veterinary Pathology, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa Vigyan Viswavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Jitendra Kumar Agrawal Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa Vigyan Viswavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Vikas Sachan Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa Vigyan Viswavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Brijesh Yadav Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa Vigyan Viswavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Anuj Kumar Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa Vigyan Viswavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India
  • Atul Saxena Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science & Animal Husbandry, Deendayal Upadhayaya Pashu Chikitsa Vigyan Viswavidyalaya Evam Go Anusandhan Sansthan, Mathura-281001, Uttar Pradesh, India

DOI:

https://doi.org/10.48165/ijvsbt.20.3.23

Keywords:

Bull semen, Carnitine, Cryopreservation, Hariana, Oxidative stress

Abstract

The goal of current investigation was to ascertain the impact of carnitine as an additive in tris-egg yolk-based extender in Hariana bull semen chosen for cryopreservation. Progressive motility, viability, HOS response, and acrosomal integrity during thawing and equilibration were estimated. The post-thaw stage of seminal plasma enzymes was assessed, and the activity of the enzymes malondialdehyde (MDA), glutathione-S-transferase (GST), and superoxide dismutase (SOD) were quantified. Four Hariana bulls provided thirty-two ejaculates, which were divided into four aliquots. Aliquot was diluted in egg yolk tris glycerol (EYTG). Group I was treated with no carnitine; Group II received 2.5 mM of carnitine; Group III received 5 mM of carnitine; and Group IV received 10 mM of carnitine/100×106 spermatozoa. When 5 mM Carnitine was supplemented into semen, it significantly (p<0.05) enhanced sperm viability, intact acrosomes, and intact membranes. This was observed in semen tested after equilibration and post-thaw stage. The addition of 5 mM and 2.5 mM carnitine reduced oxidative stress considerably (p<0.05). The results of this investigation revealed that carnitine has cryoprotective properties on bull spermatozoa via reducing oxidative stress and free radicals. To improve post-thaw sperm functional dynamics, it was suggested that 5 mM of carnitine be added as an additive to semen extender. 

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Published

2024-05-10

Issue

Vol. 20 No. 3 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) 2024

Section

Research Article

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Copyright (c) 2024 Indian Journal of Veterinary Sciences and Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Yadav, B.K., Gangwar, K., Agrawal, .J.K., Sachan, V., Yadav, B., Kumar, .A., & Saxena, A. (2024). Effect of Carnitine on Physico-Morphological Properties of Cryopreserved Hariana Bull Spermatozoa. Indian Journal of Veterinary Sciences and Biotechnology, 20(3), 116–119. https://doi.org/10.48165/ijvsbt.20.3.23
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