Effect of Carnitine on Physico-Morphological Properties of Cryopreserved Hariana Bull Spermatozoa
DOI:
https://doi.org/10.48165/ijvsbt.20.3.23Keywords:
Bull semen, Carnitine, Cryopreservation, Hariana, Oxidative stressAbstract
The goal of current investigation was to ascertain the impact of carnitine as an additive in tris-egg yolk-based extender in Hariana bull semen chosen for cryopreservation. Progressive motility, viability, HOS response, and acrosomal integrity during thawing and equilibration were estimated. The post-thaw stage of seminal plasma enzymes was assessed, and the activity of the enzymes malondialdehyde (MDA), glutathione-S-transferase (GST), and superoxide dismutase (SOD) were quantified. Four Hariana bulls provided thirty-two ejaculates, which were divided into four aliquots. Aliquot was diluted in egg yolk tris glycerol (EYTG). Group I was treated with no carnitine; Group II received 2.5 mM of carnitine; Group III received 5 mM of carnitine; and Group IV received 10 mM of carnitine/100×106 spermatozoa. When 5 mM Carnitine was supplemented into semen, it significantly (p<0.05) enhanced sperm viability, intact acrosomes, and intact membranes. This was observed in semen tested after equilibration and post-thaw stage. The addition of 5 mM and 2.5 mM carnitine reduced oxidative stress considerably (p<0.05). The results of this investigation revealed that carnitine has cryoprotective properties on bull spermatozoa via reducing oxidative stress and free radicals. To improve post-thaw sperm functional dynamics, it was suggested that 5 mM of carnitine be added as an additive to semen extender.
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