Phylogenetic Analysis and Antigenic Epitope Profiling of Glycoprotein E Gene of Bovine Herpesvirus-1

Authors

  • Diksha Panwar Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India
  • Aman Kumar Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India
  • Neha Singh Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India
  • Anubha Sharma Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India
  • Sushila Maan Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India

DOI:

https://doi.org/10.48165/ijvsbt.20.1.08

Keywords:

Antigenic epitopes, Bovine herpesvirus-1, Glycoprotein E, Peptide-based diagnostics, Phylogenetic analysis

Abstract

The current study focused on the characterization of the glycoprotein E (gE) gene of Bovine herpesvirus-1 (BoHV-1) isolates and the  prediction of antigenic epitopes for peptide-based diagnostics or vaccines. The study utilized a BoHV-1 isolate (BoHV-1/ABT/LUVAS/ HSR/2016) stored in the department which was earlier isolated from vaginal swab of aborted buffalo. The nucleotide sequencing of the gE gene showed a partial length of 689 bp, exhibiting a 98-99.85% identity with BoHV-1.1 and 1.2 strains from Indian and global sequences  available in GenBank database. Phylogenetic analysis clustered the BoHV-1 isolate with BoHV-1.1 and BoHV-1.2 strains. The deduced  amino acid sequence revealed characteristic signal sequences, conserved cysteine residues, glycosylation site, and a substitution (L→H)  at the 164th position, distinguishing it from other strains.Antigenic epitopes were predicted using various methods, including Kolaskar  & Tongaonkar Antigenicity Prediction, Bepipred, ABCpred, and SVMTrip. In total 50 antigenic epitopes were predicted of which the  Kolaskar & Tongaonkar method identified 21 antigenic peptides ranging from 6-21 amino acids in length within the 568-mer sequence. In conclusion, the present studyprovides valuable insights into the phylogenetic relation and peptide profiling of gE gene of BoHV-1.  Moreover, this study enriches the knowledge related to gE which contributes to the prevention and control strategies against BoHV-1

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Published

2023-12-22

Issue

Vol. 20 No. 1 (2024): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan-Feb ) 2024

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Panwar, D., Kumar, A., Singh, N., Sharma, A., & Maan, S. (2023). Phylogenetic Analysis and Antigenic Epitope Profiling of Glycoprotein E Gene of Bovine Herpesvirus-1 . Indian Journal of Veterinary Sciences and Biotechnology, 20(1), 35–42. https://doi.org/10.48165/ijvsbt.20.1.08
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