L-Cysteine Ameliorates Ultra-low Temperature Preservation Induced Cryodamage of Murrah Buffalo Spermatozoa

Authors

  • Kabir Alam Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Sushant Srivastava Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Daund Sushant Sakhahari Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Rajesh Kumar Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Abhishek Kumar Verma Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Sanjeev Kumar Verma Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India

DOI:

https://doi.org/10.48165/ijvsbt.19.6.27

Keywords:

Cryopreservation, Cysteine hydrochloride, Hypo-osmotic swelling test, Murrah bull, Sperm

Abstract

The present study was carried out with the aim to evaluate the effect of L-cysteine hydrochloride as an antioxidant on the in-vitro fertilizing  potentials of buffalo spermatozoa through Hypo-osmotic swelling test (HOST) and sperm mucus penetration test (CMPT). Thirty-six  ejaculates were collected from six Murrah bulls. The semen samples were divided into two groups, and were extended with Tris-citric acid-fructose-egg-yolk-glycerol (TFYG) extender without additive, i.e., control (C) extender and with L-cysteine hydrochloride (1.0 mM)  i.e. treatment (T) extender. The HOST and CMPT were evaluated at both post-dilution and post-thaw stages. The cysteine hydrochloride fortified group (T) recorded a significant (p<0.05) improvement in HOS-positive spermatozoa and the distance traveled by vanguard  spermatozoa as compared to control group. In conclusion, cysteine hydrochloride improves post-thawed sperm functional parameters  in Murrah bull semen, indicating that incorporating cysteine hydrochloride into a semen extender may improve fertility.

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References

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Published

2023-11-08

Issue

Vol. 19 No. 6 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov-Dec) 2023

Section

Short Communication

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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How to Cite

Alam, K., Srivastava, S., Sakhahari, D.S., Kumar, R., Verma, A.K., & Verma, S.K. (2023). L-Cysteine Ameliorates Ultra-low Temperature Preservation Induced Cryodamage of Murrah Buffalo Spermatozoa . Indian Journal of Veterinary Sciences and Biotechnology, 19(6), 127–129. https://doi.org/10.48165/ijvsbt.19.6.27
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