Effect of Amla (Phyllanthus emblica) Extract on Fresh and Cryopreserved Seminal Attributes of Jamnapari Buck

Authors

  • Vikas Kumar Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science And Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Sushant Srivastava Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science And Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Kabir Alam Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science And Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Rabindra Kumar Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science And Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Rajesh Kumar Department of Veterinary Gynaecology & Obstetrics, College of Veterinary Science And Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India
  • Pramod Kumar Department of Veterinary Physiology and Biochemistry, College of Veterinary Science And Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, UP-224229, India

DOI:

https://doi.org/10.48165/ijvsbt.19.6.19

Keywords:

Buck semen, Cryopreservation, Enzyme leakage, Phyllanthus emblica (Amla) as extender

Abstract

 

The aim of the present study was to determine the effect of an ethanolic extract of Phyllanthus emblica (Amla) fruit on the quality of  goat semen after the freezing-thawing process. Twelve ejaculates collected from two Jamnapari bucks were evaluated. Semen samples  were split-diluted with a Tris-based extender containing the additive ethanolic extract of Phyllanthus emblica fruit (0.6 and 0.9%) and  an extender containing no additive (control); were filled in 0.25 mL French straws, and equilibrated for 4 h at 4o C and frozen in liquid  nitrogen vapour. Frozen straws were thawed individually at 37o C for 30 s in a water bath. The samples were evaluated for sperm quality  parameters at pre-freeze (on dilution) and post-thaw stages. The freezing extender supplemented with 0.6 and 0.9% ethanolic extract of  Phyllanthus emblica fruit led to significantly higher percentages of post-thawed sperm motility, livability, and plasma membrane integrity  with reduced percentage of sperm abnormalities and seminal plasma enzyme activities in comparison to the controls (p < 0.001). The  study showed that adding 0.6% and 0.9% ethanolic extract of Phyllanthus emblica fruit to the extender (Tris-citric-acid-fructose-egg yolk-glycerol) improved the quality of post-thawed Jamnapari buck semen. The highest preservation efficiency was observed in 0.6%  ethanolic extract compared to other treatments.  

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References

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Published

2023-11-08

Issue

Vol. 19 No. 6 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov-Dec) 2023

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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How to Cite

Kumar, V., Srivastava, S., Alam, K., Kumar, R., Kumar, R., & Kumar, P. (2023). Effect of Amla (Phyllanthus emblica) Extract on Fresh and Cryopreserved Seminal Attributes of Jamnapari Buck . Indian Journal of Veterinary Sciences and Biotechnology, 19(6), 95–98. https://doi.org/10.48165/ijvsbt.19.6.19
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