Phenotypic and Molecular Characterization of Listeria Spp. from Raw Chicken Meat

Authors

  • Shubhangi R Warke Department of Veterinary Microbiology and Animal Biotechnology, T & R cell, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Seminary Hills, Nagpur-440006, India
  • Sumedha S Bobade Department of Veterinary Microbiology and Animal Biotechnology, T & R cell, Nagpur Veterinary College, Maharashtra Animal & Fishery Sciences University, Seminary Hills, Nagpur-440006, India

DOI:

https://doi.org/10.48165/ijvsbt.19.6.17

Keywords:

β-haemolysis, L. monocytogenes, PCR, PI-PLC, SBA

Abstract

The present study was aimed to find the prevalence of Listeria monocytogenes in raw chicken meat purchased across the Nagpur city.  Cultural examination of 100 raw poultry meat samples revealed 35.0 % prevalence of Listeria spp. Among them, L. grayi was predominant  (15 %) followed by L. monocytogenes (12 %), L. welshimeri (5 %), L. innocua (2 %) and L. seeligeri (1 %). A typical β-haemolysis clear zone  was exhibited by 18 listeriae isolates. Of these, 12 isolates with characteristic biochemical pattern and showing haemolytic zone. All 12  L. monocytogenes isolates expressed the PI PLC activity. Multiplex PCR study of Listeria spp. revealed that four L. monocytogenes isolates  were positive for plcA, actA, hlyA and iap genes. Amplified products of three virulence-associated genes namely, actA, hlyA and iap were  detected in five L. monocytogenes and three L. monocytogenes isolates were positive for hlyA and iap genes. L. grayi isolates revealed  the amplified product of iap gene. Two isolate of L. innocua were positive for actA and iap genes, Five L. welshimeri isolates and one  L. seeligeri isolate were positive for iap gene, lacking plcA, actA and hlyA genes. As actA and iap genes were detected in a two isolate  of L. innocua. It is clear that actA and iap genes are shared by L. monocytogenes and L. innocua. It is concluded that genes in three  different combinations were recorded in Listeria spp. and that prfA and hlyA detected by PCR distinctively in L. monocytogenes suggest  the usefulness of PCR amplification from various food samples.  

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References

Abdellrazeq, G., Kamar, A., & ElHoushy, S. (2014). Molecular Characterization of Listeria species Isolated from Frozen Fish. Alexandria Journal of Veterinary Science, 40, 1. https://doi. org/10.5455/ajvs.45443.

Centinkaya, F., Cibik, R., Soyutemiz, G.E., & Ozakin, C. (2004). Prevalence of Listeria species in chicken at the retail level. The Indian Veterinary Journal, 81, 1313-1316.

Doijad, S.P., Vaidyal, V., Garg, S., Kalekar, S., Rodrigues, J., D’Costa, D., Bhosle, S.N., & Barhuddhe, S.B. (2010). solation and characterization of Listeria species from raw and processed meats. Journal of Veterinary Public Health, 8(2). 83-88.

ISO 11290-1 (2017) International Standard : Microbiology of food chain horizontal method for the detection and enumeration

of Listeria monocytogenes and Listeria spp. Part 1 .Second Edition .1-44.

Fox, E.M., Wall, P.G., & Fanning, S. (2015). Control of Listeria species food safety at a poultry food production facility. Food Microbiology, 51, 81-85.

Inoue, S., Nakama, A., Arai, Y., Kokubo, Y., Maruyama, T., Saito, A., Yoshida, T., Terao, M., Yamamoto, S., & Kumagai, S. (2000). Prevalence and contamination levels of Listeria monocytogenes in retail foods in Japan. International Journal of Food Microbiology, 59(1-2), 73-77.

Jamshidi, A., & Zeinali, T. (2019). Significance and characteristics of Listeria monocytogenes in poultry products. International Journal of Food Science, 2019,1- 7. DOI: 10.1155/2019/7835253

Jaradat, Z.W., Schutze, G.E., & Bhunia., A.K. (2002). Genetic homogeneity among Listeria monocytogenes strains from infected patients and meat products from two geographic locations determined by phenotyping, ribotyping and PCR analysis of virulence genes. International Journal of Food Microbiology, 76(1-2), 1-10.

Kenar, B., Dilek, H., & Akkaya, L. (2006). Listeria spp. in dairy farms in the western part of central Anatolia. The Indian Veterinary Journal, 83, 4-6.

Kumar, B.N.V., Fairoze, N., Madhavaprasad, C.B., Karabasanavar, N., Kotresh, A.M., Nadoor, P., Prashant, S., Shilpa, A.G., Barbuddhe, S.B., Kurkure, N., & Chaudhary, S. (2016). Molecular characterization and antibiogram of Listeria monocytogenes

isolated from chicken and mutton of retail markets. Journal of Microbiology and Infectious Diseases, 6(2), 65-68.

Leclercq, A., Moura, A., Vales, G., Tessaud-Rita, N., Aguilhon, C., & Lecuit. M. (2019). Listeria thailandensis sp. nov. International Journal of Systematic and Evolutionary Microbiology, 69(1), 74-81.

Liu, D., Lawrence, M. L., Ainsworth, A.J., & Austin, F.W. (2007). Toward an improved laboratory definition of Listeria monocytogenes virulence. International Journal of Food Microbiology, 118(2), 101-115.

Loura, C.A.C., Almeidaand, R.C.C., & Almeida, P.F. (2005). The incidence and level of Listeria spp. and Listeria monocytogenes contamination in processed poultry at poultry processing plant. Journal of Food Safety, 25(1), 19-29.

Momtaz, H., & Yadollahi, S. (2013). Molecular characterization of Listeria monocytogenes isolated from fresh seafood samples in Iran. Diagnostic Pathology, 8, 149.

Nikas, Y. (2009). Isolation and molecular characterization of Listeria spp. from raw chicken and pork. M.V.Sc. Thesis. Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, MP, India.

Rawool, D.B., Malik, S.V.S., Barbuddhe, S.B., Shakuntala I., & Aurora, R. (2007). A multiplex PCR for detection of virulence associated genes in Listeria monocytogenes. International Journal of Food Safety, 9, 56-62.

Rothrock, M.J. Jr., Davis, M.L., Locatelli, A., Bodie, A., McIntosh, T.G., Donaldson, J.R., & Ricke, S.C. (2017). Listeria occurrence in poultry flocks: Detection and potential implications. Frontiers in Veterinary Science, 4, 125.

Sakaridis, I., Soultos, N., Iossifidou, E., Papa, A., Ambrosiadis, I., & Koidis, P. (2011). Prevalence and antimicrobial resistance of Listeria monocytogenes isolated in chicken slaughterhouses in northern Greece. Journal of Food Protection 74: 1017–1021.

Zeinali, T., Jamshidi, A., Bassami M., & Rad, M. (2017). Isolation and identification of Listeria spp. in chicken carcasses marketed in Northeast of Iran. International Food Research Journal, 24(2), 881-887.

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Published

2023-11-08

Issue

Vol. 19 No. 6 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov-Dec) 2023

Section

Articles

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How to Cite

Warke, S.R., & Bobade , S.S. (2023). Phenotypic and Molecular Characterization of Listeria Spp. from Raw Chicken Meat . Indian Journal of Veterinary Sciences and Biotechnology, 19(6), 86–89. https://doi.org/10.48165/ijvsbt.19.6.17
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