Gene Regulatory Pathways Associated with Maintenance of Acrosomal Integrity in Bovine Sperm
DOI:
https://doi.org/10.48165/ijvsbt.19.6.16Keywords:
Acrosome integrity, Acrosome pathways, Acrosome related transcripts, Bull fertility, CCDC174Abstract
The objective of this study was to identify the sperm-expressed genes and their pathways in maintaining acrosomal integrity for elucidating the regulatory genes involved in determining bull fertility. Semen samples collected from Holstein-Friesian bulls (n=12) were analyzed for acrosomal integrity and were grouped into high acrosomal integrity (n=6) and low acrosomal integrity (n=6). In these sperm samples, total RNA was isolated and subjected to whole transcriptome profiling using Illumina platform. Bioinformatic analyses led to the identification of 1601 up-regulated (TMCO2, GTSF1, SWI5, CCDC174 and CABS1) and 567 down-regulated (MYCB, RPS21 and PPIA) genes. The study also validated the expression levels of CCDC174 and TMCO2 using real-time PCR. The pathways [log2 (p-value)] such as spermatid development (12.17), protein transport (9.57) and mitochondrial electron transport (10.20) were enriched in the up-regulated genes. These identified pathways and their associated transcripts in high acrosome integrity group suggest that these pathways are essential for maintaining acrosome integrity and sperm fertility in bulls.
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Li, H., Zhang, H., Huang, G., Dou, Z., Xie, Y., Si, J., & Di, C. (2020). Heavy ion radiation-induced DNA damage mediates apoptosis via the Rpl27a-Rpl5-MDM2-p53/E2F1 signaling pathway in mouse spermatogonia. Ecotoxicology and Environmental Safety, 201, 110831.
Lin, Y.N., Roy, A., Yan, W., Burns, K.H., & Matzuk, M.M. (2007). Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis. Molecular and Cellular Biology, 27(19), 6794-6805.
Livak, K.J., & Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25(4), 402-408.
McReynolds, S., Dzieciatkowska, M., Stevens, J., Hansen, K.C., Schoolcraft, W.B., & Katz-Jaffe, M.G. (2014). Toward the identification of a subset of unexplained infertility: A sperm proteomic approach. Fertility and Sterility, 102(3), 692-699.
Parthipan, S., Selvaraju, S., Somashekar, L., Kolte, A. P., Arangasamy, A., & Ravindra, J.P. (2015). Spermatozoa input concentrations and RNA isolation methods on RNA yield and quality in bull (Bos taurus). Analytical Biochemistry, 482, 32-39.
Parthipan, S., Selvaraju, S., Somashekar, L., Arangasamy, A., Sivaram, M., & Ravindra, J.P. (2017). Spermatozoal transcripts expression levels are predictive of semen quality and conception rate in bulls (Bos taurus). Theriogenology, 98, 41-49.
Priyanka, P.P., & Yenugu, S. (2021). Coiled-coil domain-containing (CCDC) proteins: Functional roles in general and male reproductive physiology. Reproductive Sciences, 28(10), 2725-2734.
Reina, S., Guarino, F., Magrì, A., & De Pinto, V. (2016). VDAC3 as a potential marker of mitochondrial status is involved in cancer and pathology. Frontiers in Oncology, 6, 264.
Selvaraju, S., Ravindra, J.P., Ghosh, J., Gupta, P.S.P., & Suresh, K.P. (2008). Evaluation of sperm functional attributes in relation
to in vitro sperm-zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality. Animal Reproduction Science, 106(3-4), 311-321.
Selvaraju, S., Ramya, L., Parthipan, S., Swathi, D., Binsila, B. K., & Kolte, A. P. (2021a). Deciphering the complexity of sperm transcriptome reveals genes governing functional membrane and acrosome integrities potentially influence fertility. Cell and Tissue Research, 385, 207-222.
Selvaraju, S., Swathi, D., Ramya, L., Lavanya, M., Archana, S.S., & Sivaram, M. (2021b). Orchestrating the expression levels of sperm mRNAs reveals CCDC174 as an important determinant of semen quality and bull fertility. Systems Biology in Reproductive Medicine, 67(1), 89-101.
Sreenivasa, G., Vineeth, V. S., Kavitha, P., & Malini, S. S. (2012). Evaluation of acrosome intactness status in male infertility in Mysore, South India. International Journal of Applied and Basic Medical Research, 2(1), 31.
Sugihara, T., Wadhwa, R., Kaul, S.C., & Mitsui, Y. (1999). A novel testis specific metallothionein-like protein, tesmin, is an early marker of male germ cell differentiation. Genomics, 57(1), 130-136.
Teves, M.E., Roldan, E.R., Krapf, D., Strauss III, J.F., Bhagat, V., & Sapao, P. (2020). Sperm differentiation: The role of trafficking of proteins. International Journal of Molecular Sciences, 21(10), 3702.
Tapia Contreras, C., & Hoyer-Fender, S. (2019). CCDC42 localizes to manchette, HTCA and tail and interacts with ODF1 and ODF2 in the formation of the male germ cell cytoskeleton. Frontiers in Cell and Developmental Biology, 7, 151.
Yamase, K., Tanigawa, Y., Yamamoto, Y., Tanaka, H., & Komiya, T. (2019). Mouse TMCO5 is localized to the manchette microtubules involved in vesicle transfer in the elongating spermatids. PlosOne, 14(8), e0220917.
Yukitake, H., Furusawa, M., Taira, T., Iguchi-Ariga, S.M., & Ariga, H. (2002). AMAP-1, a novel testis-specific AMY-1-binding protein, is differentially expressed during the course of spermatogenesis. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1577(1), 126-132.
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