Gene Regulatory Pathways Associated with Maintenance of Acrosomal Integrity in Bovine Sperm

Authors

  • Divakar Swathi Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, India.
  • Laxman Ramya Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, India.
  • Maharajan Lavanya Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, India.
  • Santhanahalli Siddalingappa Archana Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, India.
  • Balaganur Krishnappa Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, India.
  • B Krishnan Binsila Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, India.
  • Sellappan Selvaraju Reproductive Physiology Laboratory, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Bengaluru-560030, India.

DOI:

https://doi.org/10.48165/ijvsbt.19.6.16

Keywords:

Acrosome integrity, Acrosome pathways, Acrosome related transcripts, Bull fertility, CCDC174

Abstract

The objective of this study was to identify the sperm-expressed genes and their pathways in maintaining acrosomal integrity for  elucidating the regulatory genes involved in determining bull fertility. Semen samples collected from Holstein-Friesian bulls (n=12)  were analyzed for acrosomal integrity and were grouped into high acrosomal integrity (n=6) and low acrosomal integrity (n=6). In these  sperm samples, total RNA was isolated and subjected to whole transcriptome profiling using Illumina platform. Bioinformatic analyses  led to the identification of 1601 up-regulated (TMCO2, GTSF1, SWI5, CCDC174 and CABS1) and 567 down-regulated (MYCB, RPS21 and PPIA) genes. The study also validated the expression levels of CCDC174 and TMCO2 using real-time PCR. The pathways [log2 (p-value)]  such as spermatid development (12.17), protein transport (9.57) and mitochondrial electron transport (10.20) were enriched in the  up-regulated genes. These identified pathways and their associated transcripts in high acrosome integrity group suggest that these  pathways are essential for maintaining acrosome integrity and sperm fertility in bulls.  

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References

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Published

2023-11-08

Issue

Vol. 19 No. 6 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Nov-Dec) 2023

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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How to Cite

Swathi, D., Ramya, L., Lavanya, M., Archana, S.S., Krishnappa, B., Binsila, B.K., & Selvaraju, S. (2023). Gene Regulatory Pathways Associated with Maintenance of Acrosomal Integrity in Bovine Sperm . Indian Journal of Veterinary Sciences and Biotechnology, 19(6), 80–85. https://doi.org/10.48165/ijvsbt.19.6.16
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