Therapeutic efficacy of ozone insufflations in alleviating endometritis in cows

Bijay Kumar  Patra; Indramani  Nath; Kuresh Kumar  Nayak; Rajshree  Mishra; Chinmay  Mishra; Shuvranshu Shek har  Biswal; Anjan Kumar  Sahoo; Sunil Manohar  Parida

doi:10.48165/ijar.2023.44.01.12

Authors

Bijay Kumar Patra Department of Animal Reproduction, Gynaecology and Obstetrics ,College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003
Indramani Nath Department of Veterinary Surgery and Radiology ,College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003
Kuresh Kumar Nayak Department of Animal Reproduction, Gynaecology and Obstetrics ,College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003
Rajshree Mishra Department of Department of Veterinary Microbiology ,College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003
Chinmay Mishra Department of Animal Breeding and Genetics College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003
Shuvranshu Shek har Biswal Department of Animal Reproduction, Gynaecology and Obstetrics ,College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003
Anjan Kumar Sahoo Department of Veterinary Surgery and Radiology ,College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003
Sunil Manohar Parida Department of Animal Reproduction, Gynaecology and Obstetrics ,College of Veterinary Science & Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003

DOI:

https://doi.org/10.48165/ijar.2023.44.01.12

Keywords:

Bacterial load, Endometritis, Ozone therapy

Abstract

The present investigation was carried out to study the effect of intrauterine ozone insufflation alone or in combination with cephalexin in treating the postpartum clinical endometritis in dairy cows. A total number of fourteen postpartum clinical endometritic cows selected after screening were allotted randomly into 2 equal groups (n=7). The Group I & II cows were intrauterinely administered with ozone insufflations either alone or in combination with cephalexin (Lixen-IU®). Seven postpartum normal cyclic animals were taken as control. Following treatment the overall reduction in combined mucous character and odour both in group I (82.04%) and group II (87.25%) were similar and highly significant (p<0.01). The most predominant bacteria found was E. coli (28.57%) followed by Pseudomonas spp. (14.28%), Staphylococcus spp. (21.42%), Streptococcus spp. (21.42%), Proteus spp. (7.14%) and Acinetobacter calcoaceticus (7.14%). Overall reduction in bacterial count on pre (day 1) and post treatment (day 7) were highly significant (p<0.01) i.e. 99.60% in group I and 99.72% group II. PMN cell counts of uterine smear were significantly (p<0.01) increased from pre treatment (25.85±3.28 in group I and 22.85±2.77) in group II) to post treatment (77.71±2.86) in group I and 87.14±1.35 in group II) which indicated the infiltration of neutrophils due to action of ozone and cephalexin. The conception rates following first AI were recorded as 42.85% in group I (3/7) and 71.42% (5/7) in group II. Thus intrauterine ozone insufflation has the potential to alleviate postpartum clinical endometritis as an efficacious, cost effective treatment option and an alternative to antibiotics with an overall improvement of fertility rate in dairy cows.

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