Fertility Improvement Following Herbal Treatment in Subfertile Buffalo Bulls
DOI:
https://doi.org/10.48165/ijar.2022.43.2.12Keywords:
Bull, Semen, Libido, Herbal, SubfertilityAbstract
The present experiment was conducted to study the impact of herbal treatment on post thaw semen quality and fertility in subfertile buffalo bulls. Three Murrah subfertile buffalo bulls maintained at bull station, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, India (Latitude/Longitude, 30.55°N, 75.54° E) with the history of poor semen quality (pre-freeze individual motility < 60%, post-thaw individual motility < 40%, Viability < 70% and Abnormality > 20%) were orally supplemented with herbal mixture (Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves, Ptychopetalum olacoides bark of each 400 mg/100 kg body weight and 300 mg/100 kg body weight of Pausinystalia yohimbe bark) daily for 60 days of treatment phase. Two semen ejaculates per week per bull were collected during pre-treatment, treatment and post-treatment phases of 60 days each and extended with Tris egg yolk extender. The freezing of extended semen was carried out in a biofreezer (4°C to -15°C @-30°C/min, -15°C to -140°C @-50°C/min) followed by plunging into liquid nitrogen for storage. Post thaw semen quality was assessed in terms of individual motility, viability, total sperm abnormalities. Present study revealed that the oral treatment of subfertile buffalo bulls with Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves; Ptychopetalum olacoides bark each @ 400 mg/100 kg body weight and Pausinystalia yohimbe bark @ 300 mg/100 kg body weight for 60 days improved post thaw semen quality and fertility.
References
Ahmad, M., Nasrullah, R., Riaz, H., Sattar, A. and Ahmad, N. (2014). Changes in motility, morphology, plasma membrane and acrosome integrity during stages of cryopreservation of buck sperm. J S Afr Vet Assoc., 85(1): 972.
Andrabi, S.M.H., Ansari, M.S., Ullah, N., Anwar, M., Mehmood, A. and Akhter, S. (2008) Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Anim Reprod Sci., 104(2-4): 427–433.
Antunes, E., Gordo, W.M., de Oliveira, J.F., Teixeira, C.E., Hyslop, S. And Nucci, D.G. (2001) The relaxation of isolated rabbit Corpus Cavernosum by the Herbal Medicine Catuama® and its Constituents. Phytother Res., 15(5): 416-421.
Bhattacharya, S.K., Satyan, K.S. and Ghosal, S. (1997). Antioxidant activity of glycowithanolides from Withania somnifera. Indian J Exp Biol., 35(3): 236-239.
Bucak, M.N., Sariozkan, S. and Tuncer, P.B. (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Rumin. Res., 89(1): 24-30.
Dahiya, S.S. and Singh, P. (2013) Nutritional and Other Management Practices for Optimum Semen Production in Buffalo Bulls. Buffalo Bull., 32(1): 277-284.
Dhuley J.N. (1998). Effect of ashwagandha on lipid peroxidation in stress-induced animals. J. Ethnopharmacol, 60(2): 173-178.
Dorostkar, K., Shoushtari, S.M.A. and Khaki, A. (2014). Effects of In Vitro Zinc Sulphate Additive to The Semen Extender on Water Buffalo (Bubalusbubalis) Spermatozoa before and after Freezing. Int. J. Fertil. Steril., 8(3): 325–332.
Etuk, E.U. and Muhammad, A.A. (2009) Fertility enhancing effects of aqueous stem bark extract of Lophira lanceolata in male Spargue dawley rats. Int. J. Plant Physiol. Biochem., 1(1): 001-004.
Gallardo, C.C., Guzman, L and Maccioni, R.B. (2012). Shilajit: A Natural Phytocomplex with Potential Procognitive Activity. Int. J. Alzheimer’s Dis., 2012: 1-4.
Gauthaman, K., Adaikan, P.G. and Prasad, R.N. (2002). Aphrodisiac properties of Tr.Terrestris extract (Protodioscin) in normal and castrerted rats. Life Sci, 71(12): 1385-1396.
Gauthaman, K., Ganesan, A.P. and Prasad, R.N. (2003). Sexual effects of puncture vine (Tribulus terrestris) extract (Protodioscin): an evaluation using a rat model. J. Alternative and Complementary Med., 9(2): 257-265.
Javed, MT., Khan, A and Kausar, R. (2000) Effect of age and season on some semen parameters of Nili-Ravi buffalo (Bubalus bubalis) bulls. Veterinarski. Arhiv., 70(2): 83-94.
Kulkarni S.K. and Dhir, A. (2008) Withania somnifera: an Indian ginseng. Prog Neuropsychopharmacol. Biol. Psychiatry., 32(5): 1093–1105.
Kumar, A., Singh, J., Nanda, A.S. and Pangaonkar, G.R. (2004). Quality and fertility of post thaw sephadex filtered bull semen. Asian-Australas. J. Anim. Sci., 17(6): 755-759.
Kumar, S. and Sharma, A. (2006). Apigenin: The Anxiolytic Constituent of Turnera aphrodisiaca. Pharm. Biol., 44(2): 84-90.
Kumaresan, A., Ansari, M.R. and Abhishek, G. (2005). Modulation of post-thaw sperm functions with oviductal proteins in buffaloes. Anim. Reprod. Sci., 90(1-2): 73–84.
Lee, Y.M., Yoon, H., Park, H.M., Song, B.C. and Yeum, K.J. (2017). Implications of red Panax ginseng in oxidative stress associated chronic diseases. J. Ginseng Res., 41(2): 113-119.
Lee, T.K., Johnke, R.M., Allison, R.R., Brien, K.F.O. and Dobbs, L.J. (2005). Radioprotective potential of ginseng. Mutagenesis, 20(4): 237-243.
Leung, K.W. and Wong, A.S.T. (2010). Pharmacology of ginsenosides: a literature review. Chin. Med., 5(20): 2-7.
Liu, J.P., Liang, C., Yin, T., Wang, H., Li, Y. and Li, Z. (2004). Effects of several Chinese herbal aqueous extracts on human sperm motility in vitro. Andrologia, 36(2): 78-83.
Nair, J.S., Brar, A.S., Ahuja, C.C., Sangha, S.P.S. and Chaudhary, K.C. (2006). A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Anim. Reprod. Sci., 96(1-2): 21-29.
Neha., Ansari, M.M. and Khan, H.A. (2017). Yohimbine hydrochloride ameliorates collagen type-II-induced arthritis targeting oxidative stress and inflammatory cytokines in Wistar rats. Enviro. Toxicol., 32(2): 619-629.
Nita, M. and Grzybowski, A. (2016). The Role of the Reactive Oxygen Species and Oxidative Stress in the Path mechanism of the Age-Related Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults. Oxid. Med. Cell Longev., 2016(12):1-23
Parandin, R., Yousofvand, N. And Ghorbani, R. (2012). The enhancing effects of alcoholic extract of Nigella sativa seed on fertility potential, plasma gonadotropins and testosterone in male rats. Iran. J. Reprod. Med., 10(4): 355–362.
Partyka, A., Rodak, O., Bajzert, J., Kochan, J. And Nizanski, W. (2017). The Effect of L-Carnitine, Hypotaurine, and Taurine Supplementation on the Quality of Cryopreserved Chicken Semen. Biomed. Res. Int., 2017: 1-9.
Patel, H.A., Siddiquee, G.M., Dinesh, V, Chaudhari. and Vishal S, Suthar. (2016). Effect of different antioxidant additives in semen diluent on cryopreservability (−196°C) of buffalo semen. Vet. World, 9(3): 299–303.
Rege, A, Juvekar P. And Juvekar, A. (2012). In Vitro Antioxidant and Anti-Arthritic Activities of Shilajit. Int. J. Pharm. Pharm. Sci., 4(2): 650-653.
Ronde, W.D. and Jong, F.H. (2011). Aromatase inhibitors in men: effects and therapeutic options. Reprod. Biol. and Endocrinol. 9(93): 1-7.
Shamsi, M.B., Venkatesh, S., Kumar, R., Gupta, N.P., Malhotra, N., Singh, N., Mittal, S., Arora, S., Arya, DS., Talwar, P., Sharma, RK. and Dada, R. (2010). Antioxidant Levels in Blood and Seminal Plasma and Their Impact on Sperm Parameters in Infertile Men. Indian J. Biochem. Biophys., 47(1): 38-43.
Sharma, M., Yaqoob, B., Singh, A., Sharma, N and Rawat, S. (2017). Effect of Temperature Humidity Index on Semen Quality of Bovine Bull. Int. J. Curr. Microbiol. App. Sci., 6(12): 1822-1830.
Sharma, P., Jha, J., Shrinivas, V., Dwivedi, L.K., Suresh, P. and Sinha, M. (2003). Shilajit: evalution of its effects on blood chemistry of normal human subjects. Anc. Sci. Life, 23(2): 114-119.
Shivkumar., Kumar, A., Honparkhe, M., Singh, A., Malhotra, P., Gandotra,, V. And Singh, P. (2018c). Effects of dietary supplementation of herbs on semen quality and oxidative stress in subfertile buffalo bulls. Int. J. Livestock Res., 8(7): 56-66.
Shivkumar., Kumar, A., Honparkhe, M., Singh, A.K. and Brar, P S. (2018b). Effects of dietary herbal supplementation on CASA based sperm motion traits in subfertile buffalo bulls (Bubalus bubalis). Int. J. Curr. Microbiol. App. Sci., 7(03): 2543-2550.
Shivkumar., Kumar, A., Singh, A.K., Honparkhe, M., Singh, P. and Malhotra, P. (2018a). Improvement in post-thaw semen quality by minimizing the lipid peroxidation following herbal treatment in sub fertile buffalo bulls. Pharma. Innov. J., 7(5): 240-244.
T'sjoen, G.G., Giagulli, V.A., Delva, H., Crabbe, P., De Bacquer, D. And Kaufman, J.M. (2005). Comparative assessment in young and elderly men of the gonadotropin response to aromatase inhibition. J. Clinical Endocrinol. Metabol., 90(10): 5717-5722.
Zhao, J., Dasmahapatra, A.K., Khan, S.I. and Khan, I.A. (2008). Anti-aromatase activity of the constituents from Turnera diffusa (Turnera diffusa). J. Ethnopharmacol., 120(3): 387-393.
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