Comparison of hormonal levels in ovarian follicular fluid of normal versus subfertile buffaloes

Mrigank Honparkhe; Vinod Kumar  Gandotra; Sarvpreet Singh  Ghuman; J S Matharoo; Parkash  Singh  Brar

doi:10.48165/ijar.2022.43.2.10

Authors

Mrigank Honparkhe Department of Veterinary Gynaecology and Obstetrics,Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
Vinod Kumar Gandotra Department of Veterinary Gynaecology and Obstetrics,Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
Sarvpreet Singh Ghuman Department of Veterinary Gynaecology and Obstetrics,Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
J S Matharoo Department of Animal Genetics and Breeding Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
Parkash Singh Brar Department of Veterinary Gynaecology and Obstetrics,Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India

DOI:

https://doi.org/10.48165/ijar.2022.43.2.10

Keywords:

Estradiol, follicular fluid, IGF-I, Progesterone

Abstract

Six normal cycling and fifteen subfertile buffaloes were subjected to transvaginal ultrasound guided aspiration of ≥5 mm size follicles and the follicular fluid was collected on each occasion to estimate the levels of progesterone, estradiol and IGF-1. The follicles were categorized and pooled according to their size as 5 to 6, >6-8 and >8 mm. In normal cycling buffaloes, estradiol and IGF concentration increased (P<0.05) as the follicular size increase whereas the progesterone concentration varied non-significantly among the different size categories of follicles. In subfertile buffaloes, the concentrations of progesterone, estradiol and IGF-1 remained similar among different follicular diameter ranges. However, the concentrations of various hormones in different size categories were significantly (P<0.05) higher in subfertile than that in normal cycling buffaloes. In conclusion, subfertile buffaloes had unchanged concentrations of hormones among different size categories indicating disturbed steroidogenesis and require further investigations.

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