Fertility Response Following Estradoublesynch and Progesterone Based Ovsynch Protocols in Delayed Pubertal Buffalo Heifers

Authors

  • Suresh K. Pothireddy Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
  • Mrigank Honparkhe Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
  • Ankit Kumar Ahuja DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Agriculture University, Palampur
  • Shahbaz Singh Dhindsa Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
  • Prahlad Singh Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India

DOI:

https://doi.org/10.48165/ijar.2022.43.1.12

Keywords:

Delayed Puberty, Buffalo, Heifers, Estradoublesynch, CIDR, Ovsynch

Abstract

The study evaluated the fertility response following Estradoublesynch and progesterone-based Ovsynch protocols in delayed pubertal buffalo heifers. A total of twenty delayed pubertal buffalo heifers were subdivided in two equal subgroups. Group I animals were treated with PGF2α analogue (500 µg cloprostenol sodium) i.m. on day 0, GnRH analogue (20 µg buserelin acetate) on day 2, PGF2α analogue (500 µg cloprostenol sodium) repeated on day 9 and estradiol benzoate 1 mg intramuscular (i.m.) on day 10. Fixed-time artificial insemination (FTAI) was done at 48 and 60h. Group II animals were subjected to GnRH analogue (20 µg buserelin acetate) i.m. along with CIDR insert intravaginally on day 0, PGF2α analogue (500 µg cloprostenol sodium) i.m. and removal of CIDR on day 7, GnRH analogue on day 9 followed by FTAI at 12 and 24h. Blood collection (plasma P4) and ultrasonography (luteal and follicular dynamics) was performed on day 0, 9, 10, 12 in group I and day 0, 7, 9 and 10 in group II animals. Pregnancy diagnosis was made at 45-60 days post AI. Estrus response was observed in all the buffalo heifers of group I and II. First service conception rate was significantly higher in group I (50%) compared to group II (20%) (P<0.05). Overall pregnancy rate was almost similar in both group I and II (60% vs 70%). In conclusion, estradoublesynch and progesterone-based ovsynch protocol can effectively induce ovulatory estrus and improve pregnancy rates in delayed pubertal buffalo heifers.

References

Ahuja, A. and Parmar, D. (2017). Role of minerals in reproductive health of dairy cattle: a review. Int. J. Livest. Res., 7(10): 16-26.

Baitule, M.M., Gawande, A.P., Kumar, U., Sahatpure, S.K., Patil, M.S. and Baitule, M.M. (2016). Effect of Aegle marmelos and Murraya koenigii in treatment of delayed pubertal buffalo heifers. Vet. World., 9(12): 1375.

Bhutia, T.D. (2014). Oestrus Induction in Delayed pubertal heifers treated with different hormonal protocols. (M.V.Sc. Dissertation, Veterinary College, MAFSU, Nagpur, India). 42-43.

Borghese, A. and Mazzi, M. (2005). Buffalo population and strategies in the world. Buff. Prod. Res., 67: 1-39.

Chaudhary, N.J., Patel, D.M., Dhami, A.J., Vala, K.B., Hadiya, K.K. and Patel, J.A. (2018). Effect of Doublesynch and Estradoublesynch protocols on estrus induction, conception rate, plasma progesterone, protein, and cholesterol profile in anestrus Gir heifers. Vet. World, 11(4):542-48.

Dora, K. (2016). Induction of Ferile Estrus By Application of Different Synch Protocols in Delayed Mature Heifers (Ph.D. dissertation, Orissa University of Agriculture and Technology; Bhubaneswar).

Ghallab, R. S., and Noseir, W. M. (2018). Comparative efficiency of different CIDR protocols for treatment of postpartum anestrous in Egyptian buffaloes. Alex. J. Vet. Sci., 49(2), 149-149.

Ghuman, S., Honparkhe, M. and Singh, J. (2014). Comparison of ovsynch and progesterone-based protocol for induction of synchronized ovulation and conception rate in subestrous buffalo during low-breeding season. Iran. J. Vet. Res., 15(4): 375.

Ghuman, S.P.S., Honparkhe, M. and Singh, J. (2015). Use of a progesterone-based protocol to synchronize ovulation and optimize subsequent fertility in sub estrous buffalo. Buffalo Bull., 34(3): 327-32.

Ghuman, S.P.S., Singh, J., Honparkhe, M., Dhami, D.S., Kumar, A., Nazir, G. and Ahuja, C.S. (2012). Fertility response with four estrus synchronization regimens in prepubertal buffalo heifers. Indian J. Anim. Sci., 82(12): 1521.

Honparkhe, M., Kumar, A. and Brar, P.S. (2019). Jalvayuparivartandauran Punjab diandeshigawan ate majjhan di prajanansamasiyawan ate pashuprabandhan de aamtarike. Pashudhan Punjab, 6(2): 5-6.

Kajaysri, J., Chuokudhoung, C. and Photikanit, G. (2015). Estrus and ovulation responses in anestrus postpartum swamp buffaloes following synchronization with a controlled internal drug release device and prostaglandin F2α based protocols. Buffalo Bull., 34(3): 357-68.

Mirmahmoudi, R., Souri, M. and Prakash, B.S. (2014a). Endocrine changes, timing of ovulation, ovarian follicular growth and efficacy of a novel protocol (Estradoublesynch) for synchronization of ovulation and timed artificial insemination in Murrah buffaloes (Bubalus bubalis). Theriogenology, 81: 237-42.

Mirmahmoudi, R., Souri, M. and Prakash, B.S. (2014b). Comparison of endocrine changes, timing of ovulations, ovarian follicular growth, and efficacy associated with Estradoublesynch and Heatsynch protocols in Murrah buffalo cows (Bubalus bubalis). Theriogenology, 82: 1012-20.

Naidu, G.V., Srinivas, M., Krishna, N.V.V.H. and Prasad, V.D. (2009). Management of delayed puberty in graded Murrah heifers under field conditions-a practical approach. Buffalo Bull., 28(4): 204-06.

Patel, A.J., Patel, J.A., Dhami, A.J., Prajapati, J.P. and Parmar, S.C. (2018). Estrus induction, fertility and biochemical profile in true anestrus surti buffalo following different estrus synchronization protocols. Indian J. Anim. Rep., 39(2): 36-39.

Pothireddy, S.K., Honparkhe, M., Singh, G., Ahuja, A.K., Dhindsa, S.S. and Singh, P., (2020). Comparison of certain blood plasma biochemical profiles between normal and delayed pubertal buffalo heifers. J. Entomol. Zool., 8(5): 323-325.

Prajapati, J.P., Patel, D.M., Patel, J.A., Dhami, A.J. and Parmar, S.C. (2018). Relative Efficacy of Various Estrus Synchronization Protocols for Improving Fertility in Cyclic and Acyclic Buffaloes. Int. J. Livest. Res., 8(7): 192-200.

Senger, P.L. (1999). The luteal phase. Pathways to Pregnancy and Parturition. Current Conceptions Inc., Pullman, pp. 131-48.

Zarkar, S.M. (2011). ‘Effect of CIDR on oestrusbehaviour and conception rate in buffalo heifers’. (Ph.D. Dissertation, Veterinary College, MAFSU, Nagpur, India), pp. 63-64.

Downloads

Published

2022-05-06

Issue

Vol. 43 No. 1 (2022): The Indian Journal of Animal Reproduction (Jan-Jun)

Section

Research Article

How to Cite

Pothireddy, S.K., Honparkhe, M., Ahuja, A.K., Dhindsa, S.S., & Singh, P. (2022). Fertility Response Following Estradoublesynch and Progesterone Based Ovsynch Protocols in Delayed Pubertal Buffalo Heifers. The Indian Journal of Animal Reproduction, 43(1), 66–70. https://doi.org/10.48165/ijar.2022.43.1.12
Crossref
Scopus
Google Scholar
Europe PMC