Supplementation of Oxyrase in Extender Preserves Physico-Morphological Characteristics of Crossbred Hampshire Boar Semen

Authors

  • Rahul Katiyar ICAR Research Complex for NEH Region Umiam, Meghalaya
  • Himsikha Chakravarty ICAR Research Complex for NEH Region Umiam, Meghalaya
  • Raju Kumar Dewry ICAR Research Complex for NEH Region Umiam, Meghalaya
  • Sayed Nabil Abedin ICAR Research Complex for NEH Region Umiam, Meghalaya
  • Mahak Singh ICAR Research Complex for NEH Region, Nagaland Centre, Medziphema
  • Rupali Rautela ICAR-Cental Institute for Research on Buffaloes, Hisar, Haryana
  • Govindasamy Kadirvel ICAR Research Complex for NEH Region Umiam, Meghalaya
  • Sourabh Deori ICAR Research Complex for NEH Region Umiam, Meghalaya

DOI:

https://doi.org/10.48165/ijar.2022.43.1.11

Keywords:

Boar, semen, Oxyrase, sperm functional attributes, Hampshire

Abstract

The aim of present study was to investigate the effect of Oxyrase, an oxygen scavenger, on sperm functional attributes in extended liquid preserved boar semen. In the experiment, we studied the effect of Oxyrase supplementation on sperm progressive motility, viability, functional integrity of sperm plasma membrane and acrosome integrity in Hampshire crossbred boars. The sperm functional parameters were assessed at 0, 3 and 5 d of incubation following Oxyrase supplementation (0.125 IU/mL). The results of study revealed that Oxyrase supplementation significantly (p<0.05) improved the percentage of sperm functional parameters in comparison to non-treated group. It was concluded that Oxyrase at 0.125 IU per ml of extended liquid preserved semen has a beneficial effect on the sperm functional attributes in crossbred Hampshire boar. 

References

Bisla, A., Honparkhe, M. and Srivastava, N. (2022). A review on applications and toxicities of metallic nanoparticles in mammalian semen biology. Andrologia, e14589.

Bisla, A., Rautela, R., Yadav, V., Saini, G., Singh, P., Ngou, A.A., Kumar, A., Ghosh, S., Kumar, A., Bag, S. and Mahajan, S. (2021). Synthesis of iron oxide nanoparticles–antiubiquitin antibodies conjugates for depletion of dead/damaged spermatozoa from buffalo (Bubalus bubalis) semen. Biotech. App. Biochem., 68(6): 1453-1468.

Bisla, A., Rautela, R., Yadav, V., Singh, P., Kumar, A., Ghosh, S., Kumar, A., Bag, S., Kumar, B. and Srivastava, N. (2020). Nano‐purification of raw semen minimises oxidative stress with improvement in post‐thaw quality of buffalo spermatozoa. Andrologia, 52(9): e13709.

Chakrabarty, J., Banerjee, D., Pal, D., De, J., Ghosh, A. and Majumder, G.C. (2007). Shedding off specific lipid constituents from sperm cell membrane during cryopreservation. Cryobiology, 54(1): 27-35.

Feng, C., Zhu, Z., Bai, W., Li, R., Zheng, Y., Tian, X., Wu, D., Lu, H., Wang Y and Zeng, W. (2020). Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine. Animals, 10(9): 1549.

Johnson, L.A., Weitze, K.F., Fiser, P. and Maxwell, W.M. (2000). Storage of boar semen. Anim. Reprod. Sci., 62(1-3):143-172.

Kadirvel, G., Kumaresan, A., Das, A., Bujarbaruah, K.M., Venkatasubramanian, V. and Ngachan, S.V. (2013). Artificial insemination of pigs reared under smallholder production system in northeastern India: success rate, genetic improvement, and monetary benefit. Trop. Anim. Health. Prod., 45(2): 679-686.

Katiyar, R., Ghosh, S.K., Karikalan, M., Kumar, A., Pande, M., Gemeda, A.I., Rautela, R., Dhara, S.K., Bhure, S.K., Srivastava, N., Patra, M.K., Chandra, V., Devi, H.L. and Singh, M. (2022a). An evidence of Humanin-like peptide and Humanin mediated cryosurvival of spermatozoa in buffalo bulls. Theriogenology, 194: 13-26.

Katiyar, R., Ghosh, S.K., Kumar, A., Pande, M., Gemeda, A.E., Rautela, R., Bhure, S.K., Dhara, S.K., Mathesh, K., Srivastava, N. and Patra, M.K. (2022b). Cryoprotectant with a mitochondrial derived peptide, humanin, improves post-thaw quality of buffalo spermatozoa. Cryo Letters, 43(1): 32-41.

Kumar, A., Ghosh, S. K., Katiyar, R., Gemeda, A.E., Rautela, R., Bisla, A., Srivastava, N., Bhure, S. K., Devi, H.L. and Chandra, V. (2022). Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa. Andrology, 10(4): 775-788.

Kumar, A., Ghosh, S.K., Katiyar, R., Rautela, R., Bisla, A., Ngou, A.A., Pande, M., Srivastava, N. and Bhure, S.K. (2021). Effect of Mito-TEMPO incorporated semen extender on physico-morphological attributes and functional membrane integrity of frozen thawed buffalo spermatozoa. Cryoletters, 42(2): 111-119.

Mavi, G.K., Dubey, P.P. and Cheema, R.S. (2020). Association of antioxidant defense system with semen attributes vis-a-vis fertility in exotic and indigenous chicken breeds. Theriogenology., 144: 158-163.

Mavi, G.K., Dubey, P.P., Cheema, R.S. and Bansal, B.K. (2019a). Characterization of fertility associated sperm proteins in Aseel and Rhode Island Red chicken breeds. Anim. Reprod. Sci., 203: 94–104.

Mavi, G.K., Dubey, P.P., Cheema, R.S., Dash, S.K. and Bansal, B.K. (2019b). Evaluation of semen quality parameters and their relationship with fertility in three genetic groups of layer chicken. Indian J. Anim. Res., 53 (10): 1268-1274.

Ngou, A.A., Ghosh, S.K., Prasad, J.K., Katiyar, R., Kumar, A., Rautela, R., Bisla, A., Srivastava, N. and Kumar A. (2020). Exploring the role of E.coli derived enzyme, Oxyrase, as an oxygen scavenger to improve the cryotolerance of spermatozoa of Sahiwal bull. Cryobiology, 97: 85-92.

Rautela, R., Srivastava, N., Bisla, A., Singh, P., Kumar, A., Ngou, A.A., Katiyar, R., Ghosh, S.K. and Bag, S. (2022). Nano- depletion of morbid spermatozoa up-regulate Ca2+ channel, depolarization of membrane potential and fertility in buffalo. Cryobiology, 109: 20-29.

Singh, M., Mollier, R.T., Pongener, N., Bordoloi, L.J., Kumar, R., Chaudhary, J.K., Katiyar, R., Khan, M.H., Rajkhowa, D.J. and Mishra, V.K. (2022). Linseed oil in boar's diet during high temperature humidity index (THI) period improves sperm quality characteristics, antioxidant status and fatty acid composition of sperm under hot humid sub-tropical climate. Theriogenology, 189: 127-136.

Szymanowicz, J., Schwarz, T., Murawski, M., Małopolska, M., Oszczęda, Z., Tuz, R., Nowicki, J. and Bartlewski, P. M. (2019). Storage of boar semen at 16-18 °C in the long-term commercial extender prepared with deionized water or nanowater. Anim. Reprod., 16(4): 864-870.

Vishwanath, R. (2003). Artificial insemination: The state of the art. Theriogenology, 59: 571–584.

White, I.G. (1993). Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod. Fertil. Dev., 5(6): 639-658.

Yeste, M. (2015). Recent advances in boar sperm cryopreservation: State of the art and current perspectives. Reprod. Domest. Anim., 50(2): 71–79.

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Published

2022-05-06

How to Cite

Katiyar, R., Chakravarty, H., Dewry, R.K., Abedin, S.N., Singh, M., Rautela, R., … Deori, S. (2022). Supplementation of Oxyrase in Extender Preserves Physico-Morphological Characteristics of Crossbred Hampshire Boar Semen. The Indian Journal of Animal Reproduction, 43(1), 62–65. https://doi.org/10.48165/ijar.2022.43.1.11