Correlation between Cornification of Vaginal Epithelial Cells and Serum Progesterone Concentration during Proestrus and Estrus in She-Dogs

Authors

  • Ananya Sharma Department of Veterinary Gynaecology and Obstetrics, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur – 176062, India
  • Madhumeet Singh Department of Veterinary Gynaecology and Obstetrics, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur – 176062, India
  • Akshay Sharma Department of Veterinary Gynaecology and Obstetrics, DGCN College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur – 176062, India

DOI:

https://doi.org/10.48165/ijar.2022.43.1.10

Keywords:

Cornified cells, Linear correlation and regression, Reproduction, Serum progesterone, She-dogs

Abstract

The present study was conducted on fifteen she-dogs presented to the Department of Veterinary Gynaecology and Obstetrics, CSK Himachal Pradesh Krishi Vishvavidyalaya (CSKHPKV), Palampur for determination of mating time. Serum progesterone was estimated from the blood sample collected in the clot activator by cephalic venepuncture from she-dogs during the proestrus and estrus stages. The objective of the study was to establish a correlation between epithelial cells proportion (%) in vaginal epithelium with serum progesterone concentration (ng/mL) during proestrus, early estrus, and mid to late estrus in she-dogs. A significant positive correlation (r= 0.74; p<0.01) between cornified cells and progesterone concentration was recorded during mid to late estrus whereas the correlation was non-significantly negative (p>0.05) for non-cornified cells (r= -0.7408 to -0.5807). Also, the cornified cell proportion was significantly higher (p<0.01) during mid to late estrus as compared to proestrus and early estrus. In conclusion, the present study validated the statistical association between cornified vaginal epithelial cells and serum progesterone concentration during mid to late estrus. 

References

Bante, N.K., Sahatpure, S.K., Patil, A.D., Upadhaye, S.V., Patil, D.V. and Bawaskar, M.S. (2018).Comparative study of determination of ovulation timing in bitches. Indian J. Canine Pract., 10: 46-48.

Bouchard, G.F., Youngquist, R.S., Clark, B., Concannon. P.W. and Braun, W.F. (1991).Estrus induction in the bitch using a combination diethylstilbestrol and FSH-P. Theriogenology., 36(1): 51-65.

Concannon, P.W. (2011). Reproductive cycles of the domestic bitch. Anim. Reprod. Sci., 124(3-4): 200-210.

Dar, R.R., Bhat, M.Y., Dogra, P., Sheikh, A.A., Bhagat, R., Ali, A. and Ilyas,W. (2017). Exfoliative Vaginal Cytology (EVC) as a tool for determining breeding time in the bitch a case study. Entom. Zool. Studies., 5(6): 1446-1448.

Feldman, E.C. and Nelson, R.W. (2004). Canine and Feline Endocrinology and reproduction, 3rd ed., USA.

Fontbonne, A. (2011). Infertility in bitches and queens: Recent Advances. Rev. Bras. Reprod. Anim.., 35(2): 202-209.

Goodman, M. (2001). Ovulation timing-Concepts and controversies. Vet. Clin. North Am. Small Anim. Pract., 31: 219-235.

Kudalkar, S.D., Ingawale, M.V., Pawshe, C.H., Hajare, S.W., Ingole, R.S. and Waghmare, S.P. (2020).Efficacy of Exfoliative Vaginal Cytology and Progesterone Assay on Fertility in Bitches. World J. Vet. Sci., 8: 36-39.

Linde, C. and Karlsson, I. (1984).The correlation between the cytology of the vaginal smear and the time of ovulation in the bitch. J. Small Anim. Practice., 25: 77-82

Moxon, R., Batty, H., Irons, G., England, G.C.W. (2012).Periovulatory changes in the endoscopic appearance of the reproductive tract and teasing behavior in the bitch. Theriogenology., 78: 1907-1916.

Siregar, T.N., Melia, J., Rohaya, T.C.N., Masyitha, D., Wahyuni, S., Rosa, J., Nurhafni, P.B. and Herrialfian. (2016). Determining Proportion of Exfoliative Vaginal Cell during Various Stages of Estrus Cycle Using Vaginal Cytology Techniques in Aceh Cattle. Vet. Med. Inter., 1:1-5.

Ververidis, H.N., Boscos, C.M., Stefanakis, A. and Krambovitis, E. (2002). Use of enzyme-immunoassay for oestradiol-17beta and progesterone quantification in canine serum. Anim. Reprod. Sci., 69: 53-64.

Volkmann,D.H. (2006). The effects of storage time and temperature and anticoagulant on laboratory measurements of canine blood progesterone concentrations. Theriogenology, 66(6-7): 1583-1586.

Wright, P. and Parry, B. (1989).Cytology of the canine reproductive system. Vet. Clin. North Am. Small Anim. Pract., 19:851-874.

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Published

2022-05-06

How to Cite

Sharma, A., Singh, M., & Sharma , A. (2022). Correlation between Cornification of Vaginal Epithelial Cells and Serum Progesterone Concentration during Proestrus and Estrus in She-Dogs. The Indian Journal of Animal Reproduction, 43(1), 57–61. https://doi.org/10.48165/ijar.2022.43.1.10