Effect of α-Tocopherol Supplementation in Rooster Semen on Sperm Quality Parameters during in-Vitro Storage at 4°C
DOI:
https://doi.org/10.48165/ijar.2022.43.1.7Keywords:
Chilled Semen, α-Tocopherol, RIR breed, Sperm AttributesAbstract
The purpose of this study was to evaluate the probable effects of the α-tocopherol (Vitamin E) addition in different levels to the Tris based egg yolk plasma (T-EYP) extender in semen of Rhode Island Red roosters on spermatozoa quality during storage of semen at 4°C for 0, 24,48 and 72h. The collected semen from roosters was mixed together and extended with T-EYP. The extended pooled semen was divided into 5 treatments (T). T1 was a control group without any vitamin E addition. For T2 to T5 groups 0.5%, 1%, 2% and 3% vitamin E (w/v) was added respectively. Treatments were evaluated for sperm motility, sperm viability, morphological defect, membrane integrity and acrosome integrity after 0, 24, 48 and 72 h of incubation at 4°C. The evaluations of spermatozoa immediately after semen collection, revealed no significant differences among values the treatment groups, whereas after incubating the treatments for different spans of time, the sperm progressive motility and viability rates for groups supplemented with vitamin E were significantly (P<0.05) higher than that of the control group. In addition, morphological defect rates of spermatozoa in the groups supplemented with different levels of α-tocopherol were significantly (P<0.05) lower than the control group. It was found that 0.5% level of α-tocopherol in extended rooster semen improved the sperm motility, viability plus to reduce the morphological defect rates of the spermatozoa up to 72 h storage time at 4°C.
References
Abd El-Ghany, W. (2022). Vitamin E and its impact on poultry production: An Update. J. Hell. Vet. Med., 73(1): 3571–3582.
Allai, L., Benmoula, A., da Silva, M. M., Nasser, B., El Amiri, B., Marciane da Silva, M., El Amiri, B. (2018). Supplementation of ram semen extender to improve seminal quality and fertility rate. Anim. Reprod. Sci., 192: 6–17.
Bisla, A., Rautela, R., Yadav, V., Alex Ngou, A., Kumar, A., Ghosh, S.K., Bag, S. and Srinivastava, N. (2021a). Effect of cryopreservation on semen quality parameters in relation to lipid peroxidation and antioxidant profile in Indian buffalo. CryoLetters, 42(1): 33–38.
Bisla, A., Rautela, R., Yadav, V., Saini, G., Singh, P., Ngou, A. A., Kumar, A.,Ghosh, S., Kumar, A., Bag, S., Mahajan, S. and Srivastava, N. (2021b). Synthesis of iron oxide nanoparticles–antiubiquitin antibodies conjugates for depletion of dead/damaged spermatozoa from buffalo (Bubalus bubalis) semen. Biotechnol. Appl. Biochem., 68(6): 1453–1468.
Bisla, A., Rautela, R., Yadav, V., Singh, P., Kumar, A., Ghosh, S., Kumar, A., Bag, S., Kumar, B. and Srivastava, N. (2020). Nano-purification of raw semen minimises oxidative stress with improvement in post-thaw quality of buffalo spermatozoa. Andrologia., 52(9), e13709.
Cheema, R. S., Kaur, S., Mavi, G. K., Singh, A. K., Honparkhe, M., and Gandotra, V. K. (2021). In vitro evaluation of Labrador dog spermatozoa cryopreserved in Tris-citric acid-fructose buffer supplemented with different combinations of extracellular and intracellular cryoprotectants. Anim. Biotechnol., 32(3): 352-365.
Gholami, H., Chamani, M., Towhidi, A. and Fazeli, M.H. (2010). Effect of feeding docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen-thawed semen in Holstein bulls. Theriogenology., 74(9):1548-58.
Kaur, S., Cheema, R. S., Mavi, G. K., Honparkhe, M., Singh, A. K., and Gandotra, V. K. (2020). Assessment and comparison of four lab made tris-base extenders for preservation of Labrador retriever dog semen at 4ºC. Indian J. Biotechnol., 19: 131-139.
Kumar, A., Ghosh, S. K., Katiyar, R., Gemeda, A.E., Rautela, R., Bisla, A., Srivastava, N., Bhure, S. K., Devi, H.L. and Chandra, V. (2022). Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa. Andrology, 10(4): 775-788.
Kumar, A., Ghosh, S.K., Katiyar, R., Rautela, R., Bisla, A., Ngou, A.A., Pande, M., Srivastava, N. and Bhure, S.K. (2021). Effect of Mito-TEMPO incorporated semen extender on physico-morphological attributes and functional membrane integrity of frozen thawed buffalo spermatozoa. Cryoletters, 42(2): 111-119.
Mavi, G. K., Dubey, P. P. and Cheema, R.S. (2017). Comparison of sperm attributes in two indigenous layer breeds and their relationship with fertility. Theriogenology Insight., 7:123–129.
Mavi, G.K., Dubey, P.P. and Cheema, R. S. (2020b). Identification of sperm‐specific proteins associated with high fertility of Punjab Red and RIR x Local cross roosters with SDS‐PAGE and Immunoblotting. Reprod. Domest. Anim., 55(2): 127-136.
Mavi, G.K., Dubey, P.P. and Cheema, R.S. (2020a). Association of antioxidant defense system with semen attributes vis a vis fertility in exotic and indigenous chicken breeds. Theriogenology., 144: 158-163.
Mavi, G.K., Dubey, P.P., Cheema, R.S. and Bansal, B.K. (2019a). Characterization of fertility associated sperm proteins in Aseel and Rhode Island Red chicken breeds. Anim. Reprod. Sci., 203: 94–104.
Mavi, G.K., Dubey, P.P., Cheema, R.S., Dash, S.K. and Bansal, B.K. (2019b). Evaluation of semen quality parameters and their relationship with fertility in three genetic groups of layer chicken. Indian J. Anim. Res., 53 (10): 1268-1274.
Ngou, A.A., Ghosh, S.K., Prasad, J.K., Katiyar, R., Kumar, A., Rautela, R., Bisla, A., Srivastava, N. and Kumar A. (2020). Exploring the role of E.coli derived enzyme, Oxyrase, as an oxygen scavenger to improve the cryotolerance of spermatozoa of Sahiwal bull. Cryobiology, 97: 85-92.
Rautela, R., Srivastava, N., Bisla, A., Singh, P., Kumar, A., Ngou, A.A., Katiyar, R., Ghosh, S.K. and Bag, S. (2022). Nano- depletion of morbid spermatozoa up-regulate Ca2+ channel, depolarization of membrane potential and fertility in buffalo. Cryobiology, 109: 20-29.
Shevchenoko, A. and Simons, K. (2010). Lipidomics: Coming to grip with lipid diversity. Nature Reviews Molecul. Cell Biol., 11(8): 593-98.
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