Evaluation of polyacrylamide gel as a substitute for bovine estrual mucus for sperm migration studies in buffaloes

Authors

  • GAGANDEEP KAUSHAL Department of Animal Reproduction, Gynaecology & Obstetrics Punjab Agricultural University, Ludhiana - 141 004 (Punjab)
  • G R PANGAONKAR
  • K C CHOUDHARY

Keywords:

Polyacrylamide gel, estrual mucus, sperm migration

Abstract

Sperm penetration test was conducted by using different grades of polyacrylamide gel (PAG) i.e. 1 %, 2%, 3% and 4% as
penetration media. The effect of duration and temperature of storage of PAG on sperm penetration distance (SPD) was recorded.
The 1% and 4% PAG were found to be not suitable for sperm penetration studies, 1% being very thin and 4% being too thick.
However, 2% and 3% PAG showed results comparable to fresh oestrual mucus. The mean SPD values in 2% and 3% PAG were
20.5±0.87 and 18.80±0.60 nun/20 min, respectively. The duration and temperature of storage showed no significant effect on SPD
values after 60 days in 2% and 3% PAG. It was concluded that 2% or 3% PAG could be used as an alternative medium to evaluate the
semen quality of buffalo bulls.

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Published

2023-03-30

Issue

Vol. 26 No. 1 (2005): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

KAUSHAL, G., PANGAONKAR, G.R., & CHOUDHARY, K.C. (2023). Evaluation of polyacrylamide gel as a substitute for bovine estrual mucus for sperm migration studies in buffaloes. The Indian Journal of Animal Reproduction, 26(1), 11–13. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/6364